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Featured researches published by Kosuke Haruki.


Epidemiology and Infection | 2002

An outbreak of gastroenteritis in Osaka, Japan due to Escherichia coli serogroup O166:H15 that had a coding gene for enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1).

Zhou Z; Jun Ogasawara; Yoshikazu Nishikawa; Y. Seto; A. Helander; Atsushi Hase; N. Iritani; Hiromi Nakamura; Kentaro Arikawa; A. Kai; Y. Kamata; H. Hoshi; Kosuke Haruki

In an outbreak of gastroenteritis on 23 July 1996, in Osaka, Japan, 54 of 91 persons who had attended a meeting the previous day became ill. Escherichia coli O166:H15 was isolated from stool specimens of patients (29/33, 88%). Laboratory tests for other bacterial pathogens and viruses were negative. The E. coli 0166 organisms did not adhere to HEp-2 cells in a localized, diffuse, or enteroaggregative manner. The organisms did not express known enterotoxigenic E. coli (ETEC) colonization factors. In polymerase chain reaction tests, the bacteria did not have coding genes for shigatoxin of enterohemorrhagic E. coli (EHEC), heat-labile, or heat-stable enterotoxin of ETEC, attachment and effacement (eaeA) of EPEC, or invasion (invE) of enteroinvasive E. coli (EIEC). Consequently, they could not be assigned to any of the recognized diarrhoeagenic groups of E. coli: EPEC, ETEC, EHEC, EIEC, enteroaggregative E. coli (EAggEC), or diffusely adhering E. coli. However, the organisms possessed the EAggEC heat-stable enterotoxin (EAST1) gene. To our knowledge, this is the first report of an outbreak caused by E. coli that did not have well-characterized virulence genes other than EAST1. The isolates showed the same DNA banding pattern in pulsed-field gel electrophoresis after digestion with the restriction enzymes XbaI or NotI. Three O166:H15 strains isolated from two sporadic cases and another outbreak during 1997-8 were distinct, indicating that multiple clones have spread already. We propose that diarrhoeal specimens should be examined for E. coli possessing the EAST1 gene.


Journal of Food Protection | 2006

Molecular typing to trace Listeria monocytogenes isolated from cold-smoked fish to a contamination source in a processing plant

Hiromi Nakamura; Yuka Tokuda; Ayumi Sono; Tomoka Koyama; Jun Ogasawara; Atsushi Hase; Kosuke Haruki; Yoshikazu Nishikawa

In this study, Listeria monocytogenes contamination in a cold-smoked fish processing plant in Osaka, Japan, was examined from 2002 to 2004. A total of 430 samples were collected and divided into five categories: raw fish, materials during processing, processing equipment, environment, and finished products. A total of 59 finished products were examined throughout this study. L. monocytogenes was isolated from four of these samples during summer and autumn but was not found during winter or spring. During the warmer seasons, L. monocytogenes was more prevalent on processing equipment, especially slicing machines (8 of 54 samples in summer and autumn versus 1 of 50 samples in winter and spring). L. monocytogenes was not detected on whole skins removed from 23 frozen raw fish. L. monocytogenes strains isolated from 56 samples were characterized by serotyping, pulsed-field gel electrophoresis, and three PCR-based methods. Seventy-seven L. monocytogenes strains were recognized as contaminants of the samples: 2 distinguishable strains were identified in each of 13 samples, 3 strains were identified in 2 samples, 5 strains were identified in 1 sample, and the other 40 strains were identified in 40 samples. Combining the results from these techniques, 77 strains were classified into 13 different types. Three of these types prevailed throughout the plant, and two of the three were also isolated from final products. The DNA subtype found in the product was also found on the slicing machines. Our findings suggest that the slicing machines at this plant were the source of the product contamination. Implementing an appropriate cleaning regime for the slicing machines was effective in preventing contamination.


Diagnostic Microbiology and Infectious Disease | 2003

Usefulness of a combination of pulsed-field gel electrophoresis and enrichment culture in laboratory investigation of a foodborne outbreak due to Clostridium perfringens

Hiromi Nakamura; Jun Ogasawara; Chie Monma; Atsushi Hase; Hiroshi Suzuki; Akemi Kai; Kosuke Haruki; Yoshikazu Nishikawa

Clostridium perfringens is ubiquitous in nature and normally detectable in human stools. Therefore, it is difficult to perform specific microbiologic diagnosis in foodborne outbreaks, particularly when only a few cultures are detected from fecal specimens. Usually, it has been necessary to detect over 10(6) spores/g of fecal sample as a diagnostic criterion of diarrhea due to C. perfringens. A relatively large foodborne outbreak occurred in Osaka City, Japan in October 2001. Although C. perfringens was suspected as the causal agent, four to seven days had passed after the onset of symptoms before fecal specimens were brought into our laboratory. The positive rate obtained by direct plating was quite low (13/83). We attempted to detect the organisms using enrichment culture after 75 degrees C 20 min heat-treatment, and C. perfringens enterotoxin gene (cpe)-positive strains were isolated from 53 of 81 samples. Pulsed-field gel electrophoresis (PFGE) and serotyping showed that 36 (67.9%) of these 53 strains had indistinguishable PFGE patterns and the same serotype, TW69. Our experience indicates that the enrichment culture could be useful for laboratory confirmation of a C. perfringens foodborne outbreak if it is used with adequate molecular epidemiologic methods.


International Journal of Food Microbiology | 2004

Listeria monocytogenes isolated from cold-smoked fish products in Osaka City, Japan

Hiromi Nakamura; M Hatanaka; K Ochi; M Nagao; Jun Ogasawara; Atsushi Hase; T Kitase; Kosuke Haruki; Yoshikazu Nishikawa


Japanese Journal of Infectious Diseases | 2002

Diarrheagenic Escherichia coli isolated from stools of sporadic cases of diarrheal illness in Osaka City, Japan between 1997 and 2000: Prevalence of enteroaggregative E. coli heat-stable enterotoxin 1 gene-possessing E. coli

Yoshikazu Nishikawa; Zhou Z; Atsushi Hase; Jun Ogasawara; T Kitase; Abe N; Hiromi Nakamura; Takayuki Wada; Ishii E; Kosuke Haruki; Surveillance Team


Journal of Veterinary Medical Science | 2002

Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 from beef, pork and cattle fecal samples in Changchun, China.

Zhijiang Zhou; Yoshikazu Nishikawa; Ping Zhu; Shangyuan Hong; Atsushi Hase; T. Cheasty; Henry Smith; Minggong Zheng; Kosuke Haruki


Japanese Journal of Infectious Diseases | 1999

Entamoeba histolytica outbreaks in institutions for the mentally retarded.

Abe N; Yoshikazu Nishikawa; Yasukawa A; Kosuke Haruki


Japanese Journal of Tropical Medicine and Hygiene | 2001

EVALUATION OF ANAEROPACK® (AnP) TYPE AS TOOLS OF PLASMODIUM FALCIPARUM CULTIVATION AND DRUG SENSITIVITY TESTS

Kosuke Haruki; Fumie Kobayashi; Takashi Fujino; Toshihiro Matsui; Moriyasu Tsuji


Japanese Journal of Food Microbiology | 2000

A Microbial Survey on Frozen Chilled Foods Retailed in Osaka City

Hiromi Nakamura; Atsushi Hase; Teruyo Kitase; Yasuyuki Nishi; Takakazu Imanishi; Tomio Kohno; Hiroshi Sato; Osamu Yamanaka; Hisatoshi Kai; Kazuyoshi Uehara; Kosuke Haruki


Parasitology International | 1998

Depletion of γδ T cells abrogates a protective immunity in mice primed with an attenuated derivative of plasmodium berghei NK65

Fumie Kobayashi; M Kondo; Y Hachiman; Kosuke Haruki; Toshihiro Matsui; Takashi Fujino; Seiji Waki; Takashi Tsuruhara; Moriyasu Tsuji

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Hiromi Nakamura

National Institutes of Health

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H. Hoshi

Osaka Prefecture University

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K Ochi

Osaka City University

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