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Virology | 1987

Complete nucleotide sequence of the Japanese encephalitis virus genome RNA.

Hideo Sumiyoshi; Chisato Mori; Isao Fuke; Kouichi Morita; Satoshi Kuhara; Jun Kondou; Yo Kikuchi; Hiroshi Nagamatu; Akira Igarashi

The complete nucleotide sequence of the Japanese encephalitis virus (JEV) genome RNA was determined. The JEV genome contains 10,976 nucleotides and encodes a single long open reading frame (ORF) of 10,296 nucleotides corresponding to 3432 amino acid residues. This long polypeptide is thought to be cleaved into three structural proteins and several nonstructural proteins of the virus. The genetic location of the three structural proteins was determined by comparing the deduced amino acid sequence from the nucleotide sequence with the N-terminal amino acid sequences that were determined from the three purified structural proteins. The C-terminal region of the ORF may encode a RNA-dependent RNA polymerase which has significant sequence homology with those of other RNA viruses.


Bulletin of The World Health Organization | 1987

Studies in the development of Japanese encephalitis vaccine: expression of virus envelope glycoprotein V3 (E) gene in yeast

H. Fujita; Hideo Sumiyoshi; Chisato Mori; S Manabe; M Takagi; I Yoshida; Kouichi Morita; Isao Fuke; K. Fukai; Akira Igarashi


Archive | 1983

Antibodies against Japanese Encephalitis Virus in Bovine Sera in Nagasaki, 1981.

Keiko Bundo; Kouichi Morita; Akira Igarashi; Ken Yamashita; Masataka Aizawa; Noriaki Miura


Archive | 2005

Attenuated chimeric flavivirus bearing attenuated japanese encephalitis virus gene as backbone

Kouichi Morita; Takeshi Nabeshima; Shinichi Miyake; Toshiyuki Onishi; Isao Fuke; Toyokazu Ishikawa; Hideo Goda; Masahide Ishibashi; Michiaki Takahashi


熱帯医学 Tropical medicine | 1988

Growth Patterns of Six Strains of Japanese Encephalitis Virus

Golam Masud Mohammad Shameem; Kouichi Morita; Akira Igarashi


熱帯医学 Tropical medicine | 1991

Homology among Eleven Flavivirus by Comparative Nucleotide Sequence of Geomic RNAs and Deduced Amino Acid Sequences of Viral Proteins

Mariko Tanaka; Shozo Haishi; Yoshikazu Aira; Shintaro Kurihara; Kouichi Morita; Akira Igarashi


熱帯医学 Tropical medicine | 1994

Nucleotide and Amino Acid Sequences in The PreM Region of A Japanese Encephalitis Virus Strain Isolated from A Pool of Aedes albopictus and Ae. butleri Mosquitoes Captured in Peninsula Malaysia in 1992

Indra Vythilingam; Kouichi Morita; Akira Igarashi


熱帯医学 Tropical medicine | 1992

A Rapid Plaque Assay for Japanese Encephalitis Virus Infectivity by the Presence of Actinomycin D in Overlay Medium

Hu Daji; Kouichi Morita; Akira Igarashi


Tropical Medicine (Nagasaki) | 1990

Immunogenicity of Japanese Encephalitis Virus Envelope Glycoprotein E Prepared by Four Different Methods

Ashok Kumar Srivastava; Kouichi Morita; Akira Igarashi


熱帯医学 Tropical medicine | 1989

Production of Slowly Sedimenting and Rapidly Sedimenting Components Associated with Japanese Encephalitis Virus Envelope Glycoprotein E in Infected Cell Culture Fluids

Golam Masud Mohammad Shameem; Kouichi Morita; Keiko Bundo-Morita; Akira Igarashi

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