Kousuke Matsumoto

Histological Evaluation of Artifacts in Tongue Tissue Produced by the CO2 Laser and the Electrotome

OBJECTIVE The purpose of this study was to investigate the clinical usefulness of laser surgery in pathologic diagnosis following excisional biopsy of human oral mucosa by CO(2) laser and electrotome. BACKGROUND DATA When performing pathologic diagnosis and microscopic analysis of specimens excised by the CO(2) laser, there have been concerns about thermal denaturation of the excisional margin that may prevent diagnosis. MATERIALS AND METHODS Thirty tongue tissue samples from humans that were surgically resected using a CO(2) laser (continuous wave mode for 10 cases and pulse wave mode for 10 cases) and an electrotome (10 cases) were formalin-fixed and paraffin-embedded. These preserved specimens were then cut into thick sections and subjected to hematoxylin and eosin staining followed by microscopic assessment. RESULTS Despite the differences between the CO(2) laser and electrotome methods, similar thermal denaturation, such as carbonization, vacuolar degeneration, and elongation of nuclei, were observed at the excisional margins for both methods. Use of the CO(2) laser, particularly in pulse wave mode, reduced the amount of thermal denaturation significantly (p < 0.01) compared to the electrotome. CONCLUSION These results indicate that the CO(2) laser is better than the electrotome as a means of making excisions for performing pathologic diagnosis.

Single-stage reconstruction for buccal mucosa tumor resection including the labial commissure using a facial artery musculomucosal flap and a vermilion advancement flap

Abstract Resection of buccal mucosa tumors may occasionally involve the labial angle and peripheral skin. Reconstruction is required when the resection involves more than one-third of the lip. We describe a single-stage reconstruction using a facial artery musculomucosal (FAMM) flap and a vermilion advancement flap after buccal mucosa resection including the labial angle. A 62-year-old woman with carcinoma in situ of the buccal mucosa underwent tumor resection. The resection included the right labial angle and peripheral skin. The resection did not lead to a massive lip defect, but a wide defect resulted on the mucosal side. The defect on the mucosal side was reconstructed using a FAMM flap, while the vermilion defect was covered using a vermilion advancement flap. Reconstruction performed using the FAMM flap and vermilion advancement flap produced aesthetically and functionally satisfactory outcomes.

Clinical Study of Laser Treatment for Frenectomy of Pediatric Patients

Aim To suggest regarding the timing of oral surgery and laser treatment for frenulum abnormalities in the pediatric population. Materials and methods We investigated the sex, age, frenulum site, reason for consultation, treatment method, and prognosis of 35 patients aged 15 years or younger and who were examined at our hospital for the chief complaint of frenulum abnormality. Results A total of 21 (mean age, 6.0 years) of the 35 patients underwent frenectomy using a carbon dioxide (CO2) laser. Of these, 7 patients (mean age, 2.8 years) underwent the procedure with general anesthesia and 14 patients (mean age, 7.6 years) underwent the procedure with local anesthesia. The surgical site was the lingual frenulum in 15 patients and the maxillary labial frenulum in 6 patients. No adverse events were intraoperatively reported in any of the patients, and the procedure was quickly and safely performed. The mean postoperative follow-up period was 4.6 months, and readhesion was noted in one patient (4.8%). The most common reason cited for not undergoing frenectomy in the 14 patients (mean age, 3.4 years) was the young age of the child. Conclusion Retrospective study of pediatric patients with frenulum abnormalities demonstrated the usefulness of the CO2 laser in performing frenectomy and offered suggestions regarding the timing of this procedure. Clinical significance Frenectomy performed using a CO2 laser for pediatric patients is a useful, simple, and safe treatment method. How to cite this article Komori S, Matsumoto K, Matsuo K, Suzuki H, Komori T. Clinical Study of Laser Treatment for Frenectomy of Pediatric Patients. Int J Clin Pediatr Dent 2017;10(3):272-277.