Kozo Watanuki

Some Surface Views of the Stria Vascularis and its Adjacent Areas

The surface views of the stria vascularis and its adjacent areas are presented after a silver reaction, a glutamic dehydrogenase staining and a H-3-uridine autoradiography. Between the stria vascularis and the spiral prominence, as well as Reissners membrane, some rows of spindle-formed cells were observed on the endolymphatic surface running parallel to the stria vascularis from the base to the apex of the cochlea. The H-3-uridine autoradiography of the endolymph administration revealed distinct labels of the stria vascularis and the spiral prominence. From these results, the resorptive function of the stria vascularis and the spiral prominence is discussed.

Reissners Membrane in Human Ears

Human Reissner membranes were studied by light microscopy, using the surface preparation technique. the mesothelial cells were large, flat and elongated in shape and they were arranged in radial fashion, bent slightly toward the apex of the cochlea. the form and the arrangement of the epithelial cells became more irregular with increasing age. Epithelial clusters or epithelial bands were often found in the membranes of adults. Blood vessels were occasionally found on the mesothe-lium of Reissners membrane near the apex of the cochlea.

Morphological observations of the sensory epithelium of the Macula sacculi and utriculi in the guinea pig

ZusammenfassungDas Sinnesepithelium der Macula sacculi and utriculi wurde mit Hilfe der Silberfärbung and dem Succinodehydrogenasenachweis lichtmikroskopisch untersucht. Diese beiden Methoden sind sehr wertvoll bei der Untersuchung der Häutchenpräparate der Sinnesepithelien im Vestibularorgan. 1.Größere Sinneszellen der Striola enthalten mehr Suceinodehydrogenaseaktivität als die kleineren Zellen in der Striola and in anderen Teilen der Macula.2.Die größeren Sinneszellen des Typ I in der Striola werden meistens durch den gemeinsamen Nervenkelch innerviert.3.In der Macula sacculi Bowie utriculi gehören 2/3 aller Sinneszellen der Striola zum. Typ I.4.Außerhalb der Striola setzen sich die Sinneszellen zu 45% aus Zellen des Typ I zusammen, wahrend Typ II 550% aller Sinneszellen ausmacht.5.Es gibt keine signifikante Unterschiede zwischen der Macula sacculi und der Macula utriculi in bezug auf die relative Verteilung der Sinneszellen des Typ I und Typ II.SummaryThe sensory epithelia of the macula sacculi and utriculi were investigated lightmicroscopically with the silver reaction method and succinic dehydrogenase staining. Both methods proved to be very helpful in the study of the surface structure of the sensory epithelia of the vestibular organs. 1.The larger sensory cells in the striola contained more succinic dehydrogenase activities than the smaller cells both in the striola and other parts of the macula.2.The larger sensory cells of type I in the striola were usually innvervated by common nerve chalices.3.About two-thirds of all sensory cells in the striola were type I cells both in the sensory epithelia of the macula sacculi and utriculi.4.Outside of the striola, the type I sensory cells amounted to about 45%, and the type II cells about 55% of all sensory cells.5.There were no significant differences between the macula sacculi and the macula utriculi with respect to the relative distribution of type I and type II sensory cells.

Some Surface Views of The Inner Ear By Light Microscopy

Investigations were performed by light microscope on the surface areas of the tympanal wall of the ductus cochlearis of the guinea pig, rabbit, cat and dog. Dissected specimens of the cochlea after silver reaction were used. This staining method was very helpful in observing the structure with fine high contrast.When Huschkes teeth of the spiral limbs are viewed from above, they run from the modiolus toward the inner sulcus cells with many interposed bridges between them. The surface extensions of the interdental cells (superficial cells of the spiral limbs) make a radial mosaic pavement as a whole in the direction from the modiolus toward the inner sulcus cells with slightly increasing surface area.The free surface of the inner sulcus cell becomes smaller toward the inner supporting cells.The reticular membrane maintains its compact structure in cases in which outer or inner hair cells have failed to develop. The pattern of repair of the reticular membrane was shown.At the border between Hensens and Cl...

Surface Structure of the Ampulla of the Semicircular Canal in the Guinea Pig

Investigations were performed by light microscope on the surface area of the ampulla of the semicircular canal in the guinea pig. Besides the conventional sections, dissected specimens of the ampulla

Repair pattern of the reticular lamina of the organ of corti in human ears

The repair pattern of the reticular lamina of the organ of Corti was studied in the cochleae of human ears. The inner ears were obtained at autopsy from the individuals who had no evidence of auditory or vestibular disorders or therapy with ototoxic drugs:

A Morphological Study of the Vestibular Sensory Epithelia in the Rabbit

Surface preparations were made of the vestibular sensory end-organs of the rabbit. The total sensory cell counts and surface area measurements were made from the composite photographic reconstructions. The general topographical and cytoarchitectural arrangements were found to be similar to those previously described for guinea pigs, squirrel monkeys and humans. The sensory cell counts of the maculae are about 50% larger in the rabbit than in the guinea pig, while those of the cristae remained almost unchanged in both species of animals despite a large difference in both size and weight. The body size of the species does not seem to play a decisive role in determining the sensory cell population of the vestibular sensory end-organs. The growth of the maculae is compared with that of the cristae or vice versa seems to vary from one species to another, probably depending upon a physiological need of the species.

Treatment of Esophageal Stenosis in the Neck after Sungical Reconstruction

58才, 女性. 下咽頭腫瘍による咽頭喉頭頸部食道摘出例にをける再建頸部食道術後狭窄の治療経験をのべた.(1) 局所皮弁及び一部遊離植皮を用いる方法による食道再建であるが, 4年3ヶ月で狭窄を生じた.(2) 食道再建術後長期間を経て生じた瘻孔形成, 食道は狭小に起因する可能性をチェックする必要がある.(3) 狭窄部瘢痕を摘出したのち粘膜縫合を行い, 経鼻胃管のみを留置していても, いづれ抜管後に再狭窄を生ずる可能性がある.(4) 狭窄部の処置は, DP皮弁にて新しく食道を作り変える等の, 根本的方法をとる方がよいように思われる.(5) 症例によっては, 食物の経口摂取を可ならしめながら, 食道持続拡張プロテーゼを長期間留置 (本例では5ヶ月) する方法も考慮されてよいと思う.

Streptomycin and Kanamycin Effect upon the Vestibular Sensory Epithelia of the Maculae sacculi and utriculi in the Guinea Pig

The pathological patterns of the sensory epithelia of the maculae sacculi and utriculi were investigated after streptomycin and kanamycin administration. The silver reaction method and the succinic dehydrogenase staining were used for the surface study of the sensory epithelia. The sensory cells of type I with the common nerve chalices in the striola were most vulnerable. There was no conspicuous difference of sensitivity between the type II cells inside and outside the striola. The morphological vulnerability was emphasized in the explanation for the difference in degree of sensitivity between the type I and the type II sensory cells.

Different reactions between the type I and type II sensory cells of the vestibular organs after silver reaction

ZusammenfassungAn der Zelloberfläche waren unterschiedlich starke Silberreaktionen zwischen den Sinneszellen des Typ I and Typ II zu sehen. Die Zellen des Typ I haben ein größeres Reduktionsvermögen auf die Silberionen und zeigten daher eine stärkere Anfärbung als die des Typ II. Das könnte bedeuten, daß beide Zelltypen zur Ausführung eines gleichen mechanischen Reizes unterschiedliche Receptor-Potentiale bilden.SummaryDifferent silver reactions were detected on the hair-bearing surfaces between the type I and type II cells. After treatment with silver nitrate, the surfaces of the type I sensory cells were stained darker than those of the type II cells, indicating that the type I calls have a more effective silver-reducing property than the type If cells. This evidence presumably indicates, that the two types of cells are different in the reduction of a receptor potential for the same mechanical stimulus.An der Zelloberfläche waren unterschiedlich starke Silberreaktionen zwischen den Sinneszellen des Typ I and Typ II zu sehen. Die Zellen des Typ I haben ein größeres Reduktionsvermögen auf die Silberionen und zeigten daher eine stärkere Anfärbung als die des Typ II. Das könnte bedeuten, daß beide Zelltypen zur Ausführung eines gleichen mechanischen Reizes unterschiedliche Receptor-Potentiale bilden. Different silver reactions were detected on the hair-bearing surfaces between the type I and type II cells. After treatment with silver nitrate, the surfaces of the type I sensory cells were stained darker than those of the type II cells, indicating that the type I calls have a more effective silver-reducing property than the type If cells. This evidence presumably indicates, that the two types of cells are different in the reduction of a receptor potential for the same mechanical stimulus.