Krishna Bahadur

Photochemical Formation of Self-Sustaining Coacervates

Summary The problem of the origin of life was investigated. The precursors of the present-day cells and probable evolutions were considered and procedures used described.

Study of 2,3-butanediol formation by serratiamarcescens

SummarySerratia marcescens can be used for the fermentation of sugar for the formation of 2,3-butanediol. Presence of 1% calcium carbonate increases the formation of the diol and 0.292% of phosphate gives the maximum percentage yield of the diol. The optimumph for the formation of the diol has been found to be 7.

Microbial fixation of nitrogen in presence of lanthanum sulphate with sodium molybdate

The effect of lanthanum sulphate together with 100 micrometer of sodium molybdate show that there is considerable increase in nitrogen fixation and carbon consumption in the culture medium of nitrogen-fixing Azotobacter species A1 and A2, isolated from Allahabad soil. But these combination decrease the nitrogen fixation and carbon consumption in case of another species, Azotobacter A3, of the same soil.

Der Einfluß der Wasserstoffion-Konzentration und der Temperatur auf die Milchgerinnung und Caseinhydrolyse durch Papain

ZusammenfassungIm Papain findet sich ein milchgerinnendes Ferment; sein Wirksamkeitsbereich reicht von pH 4,2–8,0; sein pH-Optimum liegt bei pH 5,2, das Temperatur-Optimum bei 60° C. Bei steigender Temperatur wird die Gerinnungszeit abgekürzt.Das proteolytische Ferment spaltet Casein bei einem pH -Bereich von 2,2 bis 5,2. Mit steigender Temperatur wird dieses Optimum nach der sauren Seite verschoben. Die Hydrolyse erfolgt in den ersten 30 min rasch, später langsam; nach 90 min tritt eine Inaktivierung des Fermentes durch hydrolytische Abbauprodukte ein. Die Acidität der Hydrolysenflüssigkeit sinkt ab, namentlich wenn das pH < 2,2-3,2 ist..Im Papain kommt außerdem noch eine Decarboxylase vor, die außer Citronensäure auch Weinsäure und Salicylsäure decarboxyliert.

Investigation of cerium and yttrium ions effects on microbial nitrogen fixation and determination of inhibition

Critical investigation of the effect of yttrium and cerium ions in culture media for nitrogen-fixing bacteria showed strong inhibition. There was a decrease in the amount of nitrogen fixed; simultaneously, in the samples containing cerium ions, carbon consumption values increased as the molarity of metal ion was enhanced. Inhibition rate was invariably maximum at the highest concentration of metal ions.

Activatory Effect of some Rare Earths on Microbial Nitrogen Transformations

Summary Lanthanum sulphate induced enhancement of nitrogen fixation by bacteria. The carbon consumption rate was similarly increased. In three microbial specimen the rate of mg. nitrogen fixed per g. carbon consumed was greater in presence of the metal ion, while in one case the rate decreased appreciably. Praseodymium sulphate inhibited the nitrogen fixation values in one sample, while three specimen showed activation. Appreciable increase in respiratory rate was perceived in all the cases studied. Inhibition was apparent in data of nitrogen fixed/carbon consumed. Lanthanum ions clearly acted as promotors in nitrogen fixing bacteria.

Titrimetric estimation of cysteine alone and in the presence of other amino-acids

Cysteine has been determined alone and in the presence of other amino-acids by titration with mercury(II) at pH 6.2 or 9.2, and with lead at pH 9.2. In all three procedures, a 1:1 complex is formed.

Effect of uranyl acetate on microbial fixation of nitrogen in presence of lanthanum sulphate.

Summary 100 μM of uranyl acetate increased the fixation of nitrogen in presence of 25 μM of lanthanum sulphate in the culture media. Further increase of lanthanum sulphate in the culture media, having 100 μM of uranyl acetate, shows a decrease to different extents in the three samples of microorganisms. The bacterial sample A 3 shows this decrease to a large extent.

Study of a Papain Activation Factor Present in the Dialysable Fraction of Soyabean Extract

Soyabean extract contains an activator which passes through parchment paper. The increase in the activity of a mixture of urease and papain with increasing amount of papain in the mixture is due to the increase in the non-dialysable fraction of papain.AbstractExtrakt ze semen soji obsahuje aktivátor, který prochází pergamenem. Zvýšení aktivity směsi ureázy a papainu se stoupajícím podílem papainu je způsobován zvýšením podílu nedialyzovatelné složky papainu.AbstractЭкетракт из семян сои содержит активатор, проходящий пергаменом. Повыппение актеивностн уреазы и папина с новышающейся долей папаина вызвано повышением доли недиалируемой части папаиа.

A study of the influence of zinc ions on the production of 2,3 butanediol by Pichia indica

SummaryPichia indica produces 2,3 butanediol during fermentation. The formation of this glycol increases with the presence of Zn++ in the culture. The increase of Zn++ concentration increases the formation of 2,3 butanediol in the culture till a maximum is reached. Further increase of Zn++ concentration decreases the diol production. On increasing the period of fermentation the glycol disappears from the culture.