Kristin Greco

Online Reviews of 500 Urologists

PURPOSE Patient demand for easily accessible information about physician quality has led to the development of physician review websites. These sites concern some physicians who argue that ratings can be misleading. In this study we describe the landscape of online reviews of urologists by looking at a sample of ratings and written reviews from popular physician review websites. MATERIALS AND METHODS A total of 500 urologists were randomly selected from a database of 9,940. Numerical ratings from 10 popular physician review websites were collected for each physician and analyzed. Written reviews from a single physician review website were also collected and then categorized as extremely negative/positive, negative/positive or neutral. RESULTS Our sample consisted of 471 male and 29 female urologists from 39 states including small and large cities and 4 census regions. There were 398 (79.6%) urologists who had at least 1 rating on any of the 10 physician review websites (range 0 to 64). On average the composite rating was based on scores from only 2.4 submitted ratings. Most physicians had positive ratings (86%), with 36% having highly positive ratings. No difference was seen in the median number of reviews when gender (p = 0.72), region (p = 0.87) and city size (p = 0.87) were compared. Written reviews were mostly positive or extremely positive (53%). CONCLUSIONS We advise physicians and patients to be aware that most urologists are rated on at least 1 physician review website, and while most ratings and reviews are favorable, composite scores are typically based on a small number of reviews and, therefore, can be volatile.

Metabolic Syndrome and Urinary Stone Composition: What Factors Matter Most?

OBJECTIVE To determine which metabolic syndrome (MetS) factors lead to differences in stone composition in a multivariate analysis. METHODS We retrospectively reviewed medical charts of patients who had a kidney stone removed over a 5-year period (2006-2011). MetS factors (obesity [body mass index {BMI} >30], diabetes mellitus [DM], hypertension [HTN], and dyslipidemia [DLD]) were tallied in each patient. For the latter 3 factors, medical treatment for the condition was used to tag a patient with the condition. Stone composition was determined by the dominant (>50%) component. Statistical analysis was designed to determine which MetS factors were independently associated with differences in stone composition. RESULTS Five hundred ninety kidney stones were included in the analysis. Patients with MetS had a higher prevalence of uric acid stones and lower prevalence of calcium phosphate stones. HTN and DM were independently associated with differences in composition, specifically uric acid stones (higher proportion), and calcium phosphate stones (lower proportion). Obesity was not associated with differences in composition, although a secondary analysis of morbidly obese patients showed a higher proportion of uric acid stones and a lower proportion of calcium oxalate stones. CONCLUSION HTN and DM are the MetS factors independently associated with differences in stone composition, specifically the uric acid and calcium phosphate components. Obesity has little effect on stone composition until a very high (>40) BMI is reached. The overall effect of MetS factors on stone type is relatively small, because most stones are calcium oxalate and MetS factors have little impact on calcium oxalate frequency.

Urothelial cells undergo epithelial-to-mesenchymal transition after exposure to muscle invasive bladder cancer exosomes

Bladder cancer, the fourth most common noncutaneous malignancy in the United States, is characterized by high recurrence rate, with a subset of these cancers progressing to a deadly muscle invasive form of disease. Exosomes are small secreted vesicles that contain proteins, mRNA and miRNA, thus potentially modulating signaling pathways in recipient cells. Epithelial-to-mesenchymal transition (EMT) is a process by which epithelial cells lose their cell polarity and cell–cell adhesion and gain migratory and invasive properties to become mesenchymal stem cells. EMT has been implicated in the initiation of metastasis for cancer progression. We investigated the ability of bladder cancer-shed exosomes to induce EMT in urothelial cells. Exosomes were isolated by ultracentrifugation from T24 or UMUC3 invasive bladder cancer cell conditioned media or from patient urine or bladder barbotage samples. Exosomes were then added to the urothelial cells and EMT was assessed. Urothelial cells treated with bladder cancer exosomes showed an increased expression in several mesenchymal markers, including α-smooth muscle actin, S100A4 and snail, as compared with phosphate-buffered saline (PBS)-treated cells. Moreover, treatment of urothelial cells with bladder cancer exosomes resulted in decreased expression of epithelial markers E-cadherin and β-catenin, as compared with the control, PBS-treated cells. Bladder cancer exosomes also increased the migration and invasion of urothelial cells, and this was blocked by heparin pretreatment. We further showed that exosomes isolated from patient urine and bladder barbotage samples were able to induce the expression of several mesenchymal markers in recipient urothelial cells. In conclusion, the research presented here represents both a new insight into the role of exosomes in transition of bladder cancer into invasive disease, as well as an introduction to a new platform for exosome research in urothelial cells.

Five-year experience with metallic stents for chronic ureteral obstruction.

PURPOSE We present 5 years of outcome data on metallic ureteral stents in a cohort of patients treated for chronic ureteral obstruction. MATERIALS AND METHODS We retrospectively identified and analyzed the records of all patients in whom a Resonance® Metallic Ureteral Stent was placed between early 2007 and late 2011 at our institution. We performed a descriptive analysis of key outcomes, including the failure and death rates, and stenting duration, defined as the time from initial stent placement to last stent failure or patient death. We also performed a secondary comparative analysis of patients with a benign vs malignant etiology of obstruction. RESULTS A total of 139 metallic stents were placed in 47 patients, including 27 (57%) with malignant and 20 (43%) with a benign etiology. Of the patients 15 (32%) had bilateral obstruction. Maximum followup was 59 months (mean 20, median 13, IQR 4-31). Stent failure occurred in 13 patients (28%), including 4 in the benign and 9 in the malignant group (p = 0.35). The median duration of stenting for benign and malignant obstruction was 22 (IQR 9-39) vs 7 months (IQR 3-25) (p = 0.106). Stenting duration was equivalent when controlling for the higher death rate in the malignant group. CONCLUSIONS Resonance metallic stents are an adequate management strategy for benign and malignant ureteral obstruction. A subset of patients in each group continued to do well at more than a 3-year overall duration of stenting. Failure rates were similar for benign and malignant etiologies.

PLK-1 Silencing in Bladder Cancer by siRNA Delivered With Exosomes

OBJECTIVE To use exosomes as a vector to deliver small interfering ribonucleic acid (siRNA) to silence the polo-like kinase 1 (PLK-1) gene in bladder cancer cells. MATERIALS AND METHODS Exosomes were isolated from both human embryonic kidney 293 (HEK293) cell and mesenchymal stem cell (MSC) conditioned media. Fluorescently labeled exosomes were co-cultured with bladder cancer and normal epithelial cells and uptake was quantified by image cytometry. PLK-1 siRNA and negative control siRNA were loaded into HEK293 and MSC exosomes using electroporation. An invasive bladder cancer cell line (UMUC3) was co-cultured with the electroporated exosomes. Quantitative reverse transcriptase polymerase chain reaction was performed. Protein analysis was performed by Western blot. Annexin V staining and MTT assays were used to investigate effects on apoptosis and viability. RESULTS Bladder cancer cell lines internalize an increased percentage of HEK293 exosomes when compared to normal bladder epithelial cells. Treatment of UMUC3 cells with exosomes electroporated with PLK-1 siRNA achieved successful knockdown of PLK-1 mRNA and protein when compared to cells treated with negative control exosomes. CONCLUSION HEK293 and MSC exosomes were effectively used as a delivery vector to transport PLK-1 siRNA to bladder cancer cells in vitro, resulting in selective gene silencing of PLK-1. The use of exosomes as a delivery vector for potential intravesical therapy is attractive.

Effect of renal function on urinary mineral excretion and stone composition.

OBJECTIVE To determine the effect of renal function on urinary mineral stone excretion and composition of kidney stones in patients undergoing urologic intervention for nephrolithiasis. METHODS We performed a retrospective analysis of 158 patients with 193 kidney stones who underwent endourologic intervention for nephrolithiasis. The patients were grouped by the estimated glomerular filtration rate (eGFR). The kidney stone composition and results of the 24-hour urinalysis were reported for each group. Statistical analysis was performed using the chi-square test, Kruskal-Wallis test, and analysis of variance. RESULTS The kidney stone composition profile differed significantly between the groups, with uric acid stones associated with a lower eGFR and calcium phosphate stones associated with a greater eGFR. A lower eGFR correlated with lower urine pH, lower calcium excretion, and greater oxalate excretion. Excretion of uric acid and the body mass index did not differ between groups. CONCLUSION Different degrees of renal function correlated with certain types of kidney stones. The urine pH was lower in patients with impaired renal function. Renal function might be an underappreciated factor that affects kidney stone composition and urinary mineral excretion.

Open Versus Robotic Radical Prostatectomy in Obese Men

Objectives: Robotic-assisted radical prostatectomy (RARP) has been shown to reduce blood loss, peri-operative complications and length of stay when compared to open radical prostatectomy (ORP). We sought to determine whether the reported benefits of RARP over ORP translate to obese patients. Patients and Methods: We utilized the 2009-2010 Nationwide Inpatient Sample to identify all obese men with prostate cancer who underwent ORP and RARP. Our primary outcome was the presence of a peri-operative adverse event (i.e. blood transfusion, complication, prolonged length of stay). We fit multivariable logistic regression models to examine whether RARP in obese patients was independently associated with decreased odds of all three outcomes. Results: We identified 9,108 obese patients who underwent radical prostatectomy. On multivariable analysis, the use of RARP in the obese population was not independently associated with decreased odds of developing a peri-operative complication (OR = 0.81, CI: 0.58-1.13, p = 0.209). RARP was, however, associated with decreased odds of blood transfusion (OR = 0.17, CI: 0.10-0.30, p < 0.001) and prolonged length of stay (OR = 0.28, CI: 0.20-0.40, p < 0.001). Conclusion: Our findings suggest that in obese patients, the use of RARP may reduce length of stay and blood transfusions compared to ORP. Both approaches, however, are associated with similar odds of developing a complication.

Comparison of complication rates for unilateral and bilateral percutaneous nephrolithotomy (PCNL) using a modified Clavien grading system

Whats known on the subject? and What does the study add?

Acute acalculous cholecystitis after radical cystectomy: A report of two cases

Acute acalculous cholecystitis (AAC) is a rare complication of major surgery and critical illness. We report two cases of AAC after radical cystectomy and review the previously reported cases. A 69-year-old man with high-grade urothelial cell carcinoma (UCC) of the bladder invading into the prostatic stroma underwent an uneventful radical cystoprostatectomy, urethrectomy, extended pelvic lymphadenectomy and ileal conduit. Postoperatively, he developed a prolonged ileus, which was managed with nasogastric tube decompression and total parenteral nutrition (TPN). He subsequently developed a persistent leukocytosis, tachycardia, and elevated liver transaminases. Computed tomography (CT) and ultrasonography showed gallbladder wall thickening and pericholecystic fluid without gallstones. Cholescintigraphy demonstrated non-visualization of the gallbladder, thus confirming the diagnosis of AAC. He underwent an open cholecystectomy 2 weeks after his initial surgery. Intraoperatively, the gallbladder was grossly inflamed and thickened with focal necrosis throughout the body and fundus. His subsequent recovery was uncomplicated and he was discharged home in excellent condition. A 64-year-old man with high-grade invasive UCC within a bladder diverticulum underwent an uneventful radical cystoprostatectomy, pelvic lymphadenectomy and ileal conduit. Postoperatively, he developed an ileus managed with prolonged nasogastric tube decompression and TPN. A total of 11 days after surgery, he developed tachypnea and tachycardia, and was found to have an acute pulmonary embolus. He had diffuse abdominal pain, leukocytosis and elevated transaminases. Ultrasonography and CT showed a distended and thickened gallbladder with pericholecystic fat infiltration without any stones. Cholescintigraphy again confirmed AAC. Because of impaired functional status, he was managed with a percutaneous cholecystostomy tube; however, his pain, ileus and leukocytosis persisted. A total of