Kristina L. Kendall

Effects of β-alanine supplementation and high-intensity interval training on endurance performance and body composition in men; a double-blind trial

BackgroundIntermittent bouts of high-intensity exercise result in diminished stores of energy substrates, followed by an accumulation of metabolites, promoting chronic physiological adaptations. In addition, β-alanine has been accepted has an effective physiological hydrogen ion (H+) buffer. Concurrent high-intensity interval training (HIIT) and β-alanine supplementation may result in greater adaptations than HIIT alone. The purpose of the current study was to evaluate the effects of combining β-alanine supplementation with high-intensity interval training (HIIT) on endurance performance and aerobic metabolism in recreationally active college-aged men.MethodsForty-six men (Age: 22.2 ± 2.7 yrs; Ht: 178.1 ± 7.4 cm; Wt: 78.7 ± 11.9; VO2peak: 3.3 ± 0.59 l·min-1) were assessed for peak O2 utilization (VO2peak), time to fatigue (VO2TTE), ventilatory threshold (VT), and total work done at 110% of pre-training VO2peak (TWD). In a double-blind fashion, all subjects were randomly assigned into one either a placebo (PL – 16.5 g dextrose powder per packet; n = 18) or β-alanine (BA – 1.5 g β-alanine plus 15 g dextrose powder per packet; n = 18) group. All subjects supplemented four times per day (total of 6 g/day) for the first 21-days, followed by two times per day (3 g/day) for the subsequent 21 days, and engaged in a total of six weeks of HIIT training consisting of 5–6 bouts of a 2:1 minute cycling work to rest ratio.ResultsSignificant improvements in VO2peak, VO2TTE, and TWD after three weeks of training were displayed (p < 0.05). Increases in VO2peak, VO2TTE, TWD and lean body mass were only significant for the BA group after the second three weeks of training.ConclusionThe use of HIIT to induce significant aerobic improvements is effective and efficient. Chronic BA supplementation may further enhance HIIT, improving endurance performance and lean body mass.

The effects of a pre-workout supplement containing caffeine, creatine, and amino acids during three weeks of high-intensity exercise on aerobic and anaerobic performance

BackgroundA randomized, single-blinded, placebo-controlled, parallel design study was used to examine the effects of a pre-workout supplement combined with three weeks of high-intensity interval training (HIIT) on aerobic and anaerobic running performance, training volume, and body composition.MethodsTwenty-four moderately-trained recreational athletes (mean ± SD age = 21.1 ± 1.9 yrs; stature = 172.2 ± 8.7 cm; body mass = 66.2 ± 11.8 kg, VO2max = 3.21 ± 0.85 l·min-1, percent body fat = 19.0 ± 7.1%) were assigned to either the active supplement (GT, n = 13) or placebo (PL, n = 11) group. The active supplement (Game Time®, Corr-Jensen Laboratories Inc., Aurora, CO) was 18 g of powder, 40 kcals, and consisted of a proprietary blend including whey protein, cordyceps sinensis, creatine, citrulline, ginseng, and caffeine. The PL was also 18 g of powder, 40 kcals, and consisted of only maltodextrin, natural and artificial flavors and colors. Thirty minutes prior to all testing and training sessions, participants consumed their respective supplements mixed with 8-10 oz of water. Both groups participated in a three-week HIIT program three days per week, and testing was conducted before and after the training. Cardiovascular fitness (VO2max) was assessed using open circuit spirometry (Parvo-Medics TrueOne® 2400 Metabolic Measurement System, Sandy, UT) during graded exercise tests on a treadmill (Woodway, Pro Series, Waukesha, WI). Also, four high-speed runs to exhaustion were conducted at 110, 105, 100, and 90% of the treadmill velocity recorded during VO2max, and the distances achieved were plotted over the times-to-exhaustion. Linear regression was used to determine the slopes (critical velocity, CV) and y-intercepts (anaerobic running capacity, ARC) of these relationships to assess aerobic and anaerobic performances, respectively. Training volumes were tracked by summing the distances achieved during each training session for each subject. Percent body fat (%BF) and lean body mass (LBM) were assessed with air-displacement plethysmography (BOD POD®, Life Measurement, Inc., Concord, CA).ResultsBoth GT and PL groups demonstrated a significant (p = 0.028) increase in VO2max from pre- to post-training resulting in a 10.3% and 2.9% improvement, respectively. CV increased (p = 0.036) for the GT group by 2.9%, while the PL group did not change (p = 0.256; 1.7% increase). ARC increased for the PL group by 22.9% and for the GT group by 10.6%. Training volume was 11.6% higher for the GT versus PL group (p = 0.041). %BF decreased from 19.3% to 16.1% for the GT group and decreased from 18.0% to 16.8% in the PL group (p = 0.178). LBM increased from 54.2 kg to 55.4 kg (p = 0.035) for the GT group and decreased from 52.9 kg to 52.4 kg in the PL group (p = 0.694).ConclusionThese results demonstrated improvements in VO2max, CV, and LBM when GT is combined with HIIT. Three weeks of HIIT alone also augmented anaerobic running performance, VO2max and body composition.

Effect of calcium β-hydroxy-β-methylbutyrate (CaHMB) with and without resistance training in men and women 65+ yrs: a randomized, double-blind pilot trial.

BACKGROUND Evidence suggests CaHMB may impact muscle mass and/or strength in older adults, yet no long-term studies have compared its effectiveness in sedentary and resistance training conditions. The purpose of this study was to evaluate the effects of 24 weeks of CaHMB supplementation and resistance training (3 d wk(-1)) or CaHMB supplementation only in ≥65 yr old adults. METHODS This double-blinded, placebo-controlled, trial occurred in two phases under ad libitum conditions. Phase I consisted of two non-exercise groups: (a) placebo and (b) 3 g CaHMB consumed twice daily. Phase II consisted of two resistance exercise groups: (a) placebo and resistance exercise and (b) 3 g CaHMB consumed twice daily and resistance exercise (RE). Strength and functionality were assessed in both phases with isokinetic leg extension and flexion at 60°·s(-1) and 180°·s(-1) (LE60, LF60, LE180, LF180), hand grip strength (HG) and get-up-and-go (GUG). Dual X-Ray Absorptiometry (DXA) was used to measure arm, leg, and total body lean mass (LM) as well as total fat mass (FM). Muscle Quality was measured for arm (MQ(HG)=HG/arm LM) and Leg (MQ60=LE60/leg LM) (MQ180=LE180/leg LM). RESULTS At 24 weeks of Phase I, change in LE60 (+8.8%) and MQ180 (+20.8%) for CaHMB was significantly (p<0.05) greater than that for placebo group. Additionally, only CaHMB showed significant (p<0.05) improvements in total LM (2.2%), leg LM (2.1%), and LE180 (+17.3%), though no treatment effect was observed. Phase II demonstrated that RE significantly improved total LM (4.3%), LE60 (22.8%), LE180 (21.4%), HG (9.8%), and GUG (10.2%) with no difference between treatment groups. At week 24, only CaHMB group significantly improved FM (-3.8%) and MQHG (7.3%); however there was no treatment main effect for these variables. CONCLUSION CaHMB improved strength and MQ without RE. Further, RE is an effective intervention for improving all measures of body composition and functionality.

Ingesting a pre-workout supplement containing caffeine, B-vitamins, amino acids, creatine, and beta-alanine before exercise delays fatigue while improving reaction time and muscular endurance

BackgroundThe purpose of this study was to determine the effects of the pre-workout supplement Assault™ (MusclePharm, Denver, CO, USA) on upper and lower body muscular endurance, aerobic and anaerobic capacity, and choice reaction time in recreationally-trained males. Subjective feelings of energy, fatigue, alertness, and focus were measured to examine associations between psychological factors and human performance.MethodsTwelve recreationally-trained males participated in a 3-week investigation (mean +/- SD, age: 28 +/- 5 y, height: 178 +/- 9 cm, weight: 79.2 +/- 15.7 kg, VO2max: 45.7 +/- 7.6 ml/kg/min). Subjects reported to the human performance laboratory on three separate occasions. All participants completed a baseline/familiarization day of testing that included a maximal graded exercise test for the determination of aerobic capacity (VO2max), one-rep maximum (1-RM) for bench and leg press to determine 75% of 1-RM, choice reaction tests, and intermittent critical velocity familiarization. Choice reaction tests included the following: single-step audio and visual, one-tower stationary protocol, two-tower lateral protocol, three-tower multi-directional protocol, and three-tower multi-directional protocol with martial arts sticks. Subjects were randomly assigned to ingest either the supplement (SUP) or the placebo (PL) during Visit 2. Subjects were provided with the cross-over treatment on the last testing visit. Testing occurred 20 min following ingestion of both treatments.ResultsSignificant (p < 0.05) main effects for the SUP were observed for leg press (SUP: 13 ± 6 reps, PL: 11 ± 3 reps), perceived energy (SUP: 3.4 ± 0.9, PL: 3.1 ± 0.8), alertness (SUP: 4.0 ± 0.7, PL: 3.5 ± 0.8), focus (SUP: 4.1 ± 0.6, PL: 3.5 ± 0.8), choice reaction audio single-step (SUP: 0.92 ± 0.10 s, PL: 0.97 ± 0.11 s), choice reaction multi-direction 15 s (SUP: 1.07 ± 0.12 s, PL: 1.13 ± 0.14 s), and multi-direction for 30 s (SUP: 1.10 ± 0.11 s, PL: 1.14 ± 0.13 s).ConclusionsIngesting the SUP before exercise significantly improved agility choice reaction performance and lower body muscular endurance, while increasing perceived energy and reducing subjective fatigue. These findings suggest that the SUP may delay fatigue during strenuous exercise.

Six Weeks of High-Intensity Interval Training With and Without β-Alanine Supplementation for Improving Cardiovascular Fitness in Women

Walter, AA, Smith, AE, Kendall, KL, Stout, JR, and Cramer, JT. Six weeks of high-intensity interval training with and without β-alanine supplementation for improving cardiovascular fitness in women. J Strength Cond Res 24(5): 1199-1207, 2010-The purpose of the present study was to evaluate the effects of cycle ergometry high-intensity interval training (HIIT) with and without β-alanine supplementation on maximal oxygen consumption rate (&OV0312;o2peak), cycle ergometer workload at the ventilatory threshold (VTW), and body composition. Forty-four women (mean ± SD age = 21.8 ± 3.7 years; height = 166.5 ± 6.6 cm; body mass (BM) = 65.9 ± 10.8 kg; &OV0312;o2peak = 31.5 ± 6.2 ml·kg−1·min−1) were randomly assigned to 1 of 3 groups: β-alanine (BA, n = 14) 1.5 g + 15 g dextrose powder; placebo (PL, n = 19) 16.5 g dextrose powder; or control (CON, n = 11). Testing was conducted at baseline (week 0), after 3 weeks (week 4), and after 6 weeks (week 8). &OV0312;o2peak (ml·kg−1·min−1) and VTW were measured with a metabolic cart during graded exercise tests on a corival cycle ergometer, and body composition (percent fat = % fat and fat-free mass = FFM) were determined by air displacement plethysmography. High-intensity interval training was performed on a corival cycle ergometer 3 times per week with 5 2-minute work intervals and 1-minute passive recovery with undulating intensities (90-110% of the workload recorded at &OV0312;o2peak) during each training session. &OV0312;o2peak increased (p ≤ 0.05) in the BA and PL groups at weeks 4 and 8, but did not change (p > 0.05) for the CON group. VTW increased (p ≤ 0.05) for all groups at weeks 4 and 8. Body mass increased (p ≤ 0.05) only for the BA group at weeks 4 and 8, whereas %fat decreased (p ≤ 0.05) and FFM increased (p ≤ 0.05) at weeks 4 and 8 for all groups (BA, PL, and CON). Although it is unclear why β-alanine supplementation increased BM, there was no additive effects for increasing &OV0312;o2peak beyond the PL. Overall, these results suggested that HIIT may be an effective and time-efficient method of training to improve maximal oxygen uptake.

Effects of Four Weeks of High-intensity Interval Training and Creatine Supplementation on Critical Power and Anaerobic Working Capacity in College-aged Men

Kendall, KL, Smith, AE, Graef, JL, Fukuda, DH, Moon, JR, Beck, TW, Cramer, JT, and Stout, JR. Effects of four weeks of high-intensity interval training and creatine supplementation on critical power and anaerobic working capacity in college-aged men. J Strength Cond Res 23(6): 1663-1669, 2009-The critical power test provides 2 measures, critical power (CP) and anaerobic working capacity (AWC). In theory, the CP measurement represents the maximal power output that can be maintained without fatigue, and AWC is an estimate of work capacity associated with muscle energy reserves. High-intensity interval training (HIIT) has been shown to be an effective training method for improving endurance performance, including &OV0312;O2PEAK. In addition, creatine (Cr) supplementation has been reported to improve AWC without training; however, it has shown no effect on CP. The purpose of this study was to examine the effects of 4 weeks of HIIT with Cr supplementation on CP and AWC. Forty-two recreationally active men volunteered to participate in this study. Participants were randomly assigned to 1 of 3 groups: Cr (n = 16), 10 g Cr + 10 g dextrose; placebo (PL, n = 16), 20 g dextrose; control (CON, n = 10), no treatment. Before and after supplementation, each participant performed a maximal oxygen consumption test (&OV0312;O2PEAK) on a cycle ergometer to establish peak power output (PPO). Participants then completed a CP test involving 3 exercise bouts with the workloads set as a percentage of their PPO to determine CP and AWC. After a 2-week familiarization period of training and supplementing, PPO, CP, and AWC were remeasured before an additional 4 weeks of HIIT and supplementation were completed. Training consisted of 5 sets of 2-minute exercise bouts with 1 minute rest in between performed on the cycle ergometer, with intensities based on PPO. A significant improvement in CP was observed in the Cr group (6.72% ± 2.54%), whereas PL showed no significant change (3.87% ± 2.30%), and CON significantly decreased (6.27% ± 2.38%). Furthermore, no changes in AWC were observed in any of the groups after treatment. The current findings suggest that Cr supplementation may enhance the effects of intense interval endurance training on endurance performance changes.

Tracking fat-free mass changes in elderly men and women using single-frequency bioimpedance and dual-energy X-ray absorptiometry: a four-compartment model comparison.

Background/Objectives:To compare single estimations of fat-free mass (FFM) and to track FFM using single-frequency bioelectrical impedance analysis (BIA) and dual-energy X-ray absorptiometry (DXA) compared with a four-compartment (4C) model in healthy elderly Americans.Subjects/Methods:Thirty-four men and thirty-eight women (Caucasian, ⩾65 years) were included in the study. Subjects participated in either the control group or the exercise group. All testing and training took place during the 21-week investigation. Body composition assessments using nine BIA equations, DXA and a 4C model were performed during weeks 1, 12 and 24 of the study.Results:Single estimations for DXA and BIA produced high r values (0.79–0.95) and low standard error of estimate values (1.62–3.3 kg), producing subjective ratings of ‘ideal’ for men and ‘excellent’ for women. Both DXA and two BIA equations revealed the same significance when comparing groups and times with the 4C model. Individual accuracy for tracking changes was similar among BIA equations and DXA compared with the 4C model, with a total agreement of 25% for BIA and 27% for DXA compared with the 4C model.Conclusions:The current data in combination with the reliability errors for both BIA and DXA FFM estimations suggest that individual results should be interpreted with caution if FFM changes are <5 kg. However, DXA and BIA are both valid methods that can be used interchangeably to estimate FFM at a single time point or for tracking changes in FFM in small groups (15–22) of healthy American older adults.

The Effects of Tournament Preparation on Anthropometric and Sport-Specific Performance Measures in Youth Judo Athletes

Abstract Fukuda, DH, Stout, JR, Kendall, KL, Smith, AE, Wray, ME, and Hetrick, RP. The effects of tournament preparation on anthropometric and sport-specific performance measures in youth judo athletes. J Strength Cond Res 27(2): 331–339, 2013—The purpose of this study was to characterize the adaptations imposed by 4 weeks of precompetition judo training in youth athletes. It was hypothesized that anthropometric and sport-specific performance would improve during the preparation for a junior national championship event. Twenty youth athletes (mean ± SD; chronological age: 13.1 ± 3.2 years; training age: 5.3 ± 3.5 years; judo experience: 7.8 ± 2.5 hours per week) completed pretesting and posttesting procedures. Child (12 years old; n = 8) and adolescent (13 years old; n = 12) groups were evaluated to determine the anthropometric and sport-specific performance changes caused by 4 weeks of judo training conducted in preparation for the junior national championships. The child group showed an increase in flexibility (11.5%), and the adolescent group showed a decrease in skinfold thickness (−12.2%); increased jumping power (26.7%), force (7.7%), and velocity (19.0%); and improved judo-specific ability (−5.9%), as measured by the Special Judo Fitness Test (SJFT) index. Additionally, the SJFT index for all the study participants was shown to be inversely correlated to handgrip strength (r = −0.681), rope pull performance (r = −0.545), and jump height (r = −0.503). These results support the use of preparatory judo training in the improvement of anthropometric and sport-specific measures in adolescent athletes. Furthermore, the outcomes from this study provide direction for coaches and trainers in their efforts to impact physical performance and judo skills in children and adolescents through precompetition training.

The effects of creatine loading and gender on anaerobic running capacity.

Fukuda, DH, Smith, AE, Kendall, KL, Dwyer, TR, Kerksick, CM, Beck, TW, Cramer, JT, and Stout, JR. The effects of creatine loading and gender on anaerobic running capacity. J Strength Cond Res 24(7): 1826-1833, 2010-Creatine (Cr) loading consists of short-term, high-dosage Cr supplementation and has been shown to increase intramuscular total Cr content. Increases in body weight (BW) have been shown to result from Cr loading, with differences by gender, and increased BW may impact weight-bearing exercise. The critical velocity (CV) test is used to quantify the relationship between total running distance and time to exhaustion. The CV test provides the variable, anaerobic running capacity (ARC), which is an estimate of the anaerobic energy reserves in muscle. The purpose of this study was to examine the effects of gender and Cr loading on ARC. Fifty moderately trained men and women volunteered to participate in this randomized, double-blinded, placebo (PL)-controlled, repeated-measures study. After a familiarization session, a 3-day testing procedure was conducted. A maximal oxygen consumption test (&OV0312;o2max) on a treadmill was performed on day 1 to establish the maximum velocity (Vmax) at &OV0312;o2max and to record BW. Days 2 and 3 involved treadmill running at varying percentages of Vmax. Participants were randomly assigned to either the Cr or PL group and received 20 packets of the Cr supplement (1 packet = 5 g Cr citrate, 18 g dextrose) or 20 packets of the PL (1 packet = 18 g dextrose). After consuming 4 packets daily for 5 consecutive days, the 3-day testing procedure was repeated. The male Cr loading group exhibited a 23% higher (p = 0.003) ARC compared to the PL group. Nonsignificant BW increases were found for the Cr groups. These findings suggest that Cr loading may be an effective strategy for improving ARC in men, but not in women, and revealed only nonsignificant increases in BW. Creatine loading may be used before competition by athletes to provide improvements in high-intensity, short-duration activities.

Total body water changes after an exercise intervention tracked using bioimpedance spectroscopy: A deuterium oxide comparison

BACKGROUND & AIMS Bioimpedance spectroscopy (BIS) for the estimation of total body water (TBW) has advantages over isotope dilution techniques, including cost, portability, and ease of use. The aim of the current study was to determine the validity of a BIS device (Imp SFB7) for tracking changes in overfat and obese individuals. METHODS Sixty overfat and obese men and women (27+/-8 yr, 33.41+/-3.81%fat) participated in the study. TBW was estimated using BIS and deuterium oxide (D(2)O) before and after the ten-week intervention. RESULTS Pre-and post-intervention BIS TBW estimations errors increased as body mass index (BMI), fat mass (FM), and fat-free mass (FFM) increased (p<0.05). Delta values were more accurate than pre- and post-TBW estimations (total error=1.45 L). Age significantly influenced pre- and post-TBW errors (p<0.05). Therefore, a regression equation was developed to correct for the pre- and post-BIS-estimated TBW errors: D(2)O TBW=11.478+0.743(BIS TBW)-2.429(Gender), (Men=1, Women=2). CONCLUSIONS BIS can be considered an accurate tool for tracking changes in TBW regardless of variations in BMI, FM, FFM, or age in both overfat and obese men and women (BMI>24). Employing a BIS TBW equation is suggested over the development of resistivity coefficients based on BMI, FM, FFM or age.