Krystyna Samochocka

The incorporation of Yb and Se into the brains of rats and their effect on the Zn level.

The concentrations of Yb, Se and Zn in the brains of rats were determined after the injection of ytterbium chloride and selenodiglutathione--single or together--by neutron activation analysis. Both Yb and Se were incorporated in all the investigated brain tissues and they also caused changes in the Zn concentration.

Structure and biological activity of cationic [PtLCl(DMSO)]NO3·DMSO complex containing a chelated diaminosugar: methyl-3,4-diamino-2,3,4,6-tetradeoxy-α-l-lyxopyranoside

Cationic platinum(II) complex [Pt(C(7)H(16)N(2)O(2))(DMSO)Cl]NO(3).DMSO containing one chloride anion, methyl-3,4-diamino-2,3,4,6-tetradeoxy-alpha-L-lyxopyranoside (C(7)H(16)N(2)O(2)) and dimethylsulfoxide (DMSO) molecules forming a square-plane has been prepared and characterised, both spectroscopically and by single-crystal X-ray diffraction. Biological tests performed using leukemia L1210 cells have shown that toxicity of the title complex is similar to that of cisplatin.

Vibrational studies of Pt(II) and Pd(II) complexation by 3,4-diamino lyxo-hexopyranoside

Abstract FT-IR spectra of crystalline methyl 3,4-diamino-2,3,4,6-tetradeoxy-α- l -lyxo-hexopyranoside ( 1 ) and cis -dichloro diamino lyxose complexes of palladium(II) and platinum(II) ( 2 and 3 , respectively) have been recorded, assigned and discussed in terms of structure of the investigated complexes.

Variation of Se, Zn, Co, Fe and Rb distribution in rats upon sequence of injection with SeO2 and glutathione

The contents of Se, Zn, Co, Fe and Rb in several organs of Wistar rats were determined by instrumental neutron activation analysis (INAA) after injections of SeO2 and glutathione (GSH). Se was incorporated in all the examined organs, and the efficiency of incorporation does not depend upon the sequence of injection with SeO2 and GSH. The sequence of these injections affects the contents of the other elements in all the examined organs.

Interaction of some organic platinum(II) complexes with L5178Y-R and L5178Y-S cells

The cytotoxicity and mutagenicity of three organic platinum(II) complexes 1-3 have been studied in two strains of murine lymphoma L5178Y cells. (1) [Pt(Amp)Cl] (S,N,N) (Amp, ampicillin); (2) [Pt(Met(O))Cl2] (S,N), (met(O), L-methionine sulfoxide); (3) [Pt(Met(O))(R-IDA)] (S,N) [N,N), (R-IDA, 2,4-N-(dimethylacetanilide)-iminodiacetic acid). It has been confirmed that the cytotoxic and mutagenic action of Pt-complexes depends on the structure of stable ligand and the number and structure of the leaving groups. The highest cytotoxicity and mutagenicity was found in cells treated with the dichloromethioninesulfoxideplatinum(II) complex.

The incoporation of selenium and ytterbium into the eyes of mice

The incorporation of Se and Yb into the eyes of mice has been studied. Selenodiglutathione, (GS)2Se, or ytterbium chloride, YbCl3, were injected intraperitoneally into mice: either alone, combined, or after various time intervals. Instrumental neutron activation analysis was applied as the analytical method for the determination of the levels of Se and Yb. The concentrations of both investigated elements were highest in the retinal tissue of the eye. YbCl3 influenced the distribution of Se in the eye.

Practical aspects of simultaneous determination of selenium and ytterbium by neutron activation analysis

An instrumental method for the simultaneous determination of selenium and ytterbium by thermal neutron activation analysis is described. Selenium and ytterbium were determined via the 400.7 keV photopeak of75Se and the 177 keV photopeak of169Yb. The accuracy of this instrumental method has been examined; as a result, it has been proved that this method is most exact and accurate when the gamma-ray counting is carried out after the decay of175Yb.

Influence of cysteine and selenodicysteine on the uptake of zinc by Chlorella vulgaris Beijerinck

Abstract The uptake of zinc labelled with radioactive 65 Zn in the presence of cysteine and selenodicysteine by Chlorella vulgaris was examined. The concentration of zinc ions in the medium was 20 mg per l. The uptake yield was found to be enhanced by selenodicysteine. At concentrations of 10 −7 −10 −6 M the growth rate of Chlorella vulgaris was accelerated by the latter, provided that the specific activity of 65 Zn was 3.7 MBq/l. At this specific zinc activity cysteine increased the uptake yield during the initial 50 h of the incubation process. At specific 65 Zn-activity of 55.5 MBq/l selenodicysteine and cysteine only slightly influenced the zinc uptake by Chlorella vulgaris . No increment in the biomass was observed at this specific zinc radioactivity.

USE OF INAA TO STUDY THE BIODISTRIBUTION OF PLATINUM COMPOUNDS IN ANIMALS

The concentration of platinum in various organs of mice and rat was determined, after injection of tetrachloroplatinate and dichlorodimethioninoplatinum/II/, by neutron activation analysis. It was found that these two platinum complexes have a different biodistribution pattern in animals.

Studies on the reactions of selenite ion with 1,2-dimercaptoethane or thioacetic acid

Abstract The reactions are reported between selenite and 1,2-dimercaptoethane (DME) or thioacetic acid (TAA) to form moderately stable derivatives having an enhanced absorptions in the 230–360 nm region in combining molar ratios 3:2 and 4:1, respectively. Both reactions invariably yield one product corresponding to the selenium-containing derivative of DME or TAA. The formation of products is a pH dependent process. The equilibrium constants of reactions between selenite and DME or TAA were measured.