Kulcsár G

Immunofluorescent studies on circulating lymphocytes in oral mucosal diseases.

SummaryCirculating lymphocytes of patients with recurrent herpetic, aphthous and other oral mucosal diseases, as well as of persons with intact oral mucosa were examined with direct immunofluorescence method using HVH and adenovirus hyperimmune conjugated sera. Some of the circulating lymphocytes showed a specific fluorescence both in patients and in persons with intact oral mucosa. Increased herpes-positivity was observed only in erythema exsudativum multiforme and increased adeno-positivity in patients with recurrent aphthous stomatitis, especially in cases with constantly recurring aphthae.The possible pathologic role of the circulating virus-antigen-carrier lymphocytes is presumed.ZusammenfassungEs wurden Untersuchungen mit der direkten Immunfluorescenzmethode bei Kranken mit rezidivierenden herpetischen, aphthösen und anderen Mundschleimhautkrankheiten mit Antiherpes- und Antiadenoviruskonjugaten durchgeführt. Sowohl bei den Patienten wie bei den Personen mit intakter Mundschleimhaut wurde eine spezifische Fluorescenz beobachtet. Erhöhte Adenopositivität wurde bei Patienten mit rezidivierenden, besonders mit ständig rezidivierenden Aphthen, erhöhte Herpespositivität bei Kranken mit Erythema multiforme beobachtet.Die mögliche pathologische Rolle der virusantigen-haltenden Lymphocyten wurde angenommen.

The decreased antibacterial activity of oral polymorphonuclear leukocytes coincides with the occurrence of virus-carrying oral lymphocytes and epithelial cells

Patients suffering from periodontitis or periodontosis were selected for the study. Further subdivision of these groups was based on the presence or absence of herpes and/or adenoviruses in their oral lymphocytes and epithelial cells. The phagocytic and bactericidal activities of oral leukocytes isolated from the same individuals were compared with virus carriage. In the periodontitis group, 60.5 %, and in the periodontosis group 61.5 % of patients carried viruses, while this was established only in 21.1 % of control cases. On the other hand, emigration and sulcular gathering of the less viable polymorphonuclear leukocytes was elevated but their phagocytotic activity was decreased among periodontitis patients. Bactericidal capacity was significantly lowered among those subjects who carried viruses in their cells, as compared with virusfree persons, especially in the periodontitis group. The functions of the polymorphonuclear leukocytes accumulated in the sulcus gingivalis may be modified by mediators released from the virus-carrying cells. These mediators could achieve a greater concentration locally, and the damaged leukocytes would not be able to eliminate the microbes continuously so that the accumulation of bacterial products, among them endotoxins, could lead to periodontal inflammation.

Human lymphocyte transformation with virus antigens

SummaryLymphocyte transformation tests were carried out in patients with recurrent aphthae and control persons, using heat treated Herpesvirus hominis and adenovirus antigens of different types. From the virus antigens examined, only the Herpesvirus hominis and adenovirus type 1 antigens caused blastogenic transformation in higher rate in the lymphocyte cultures of aphthous patients, than in those of the control persons. Plasma cell reaction was observed with some adenovirus types, and the appearance of polynuclear giant cells was detected besides the lymphoblasts in the lymphocyte cultures of certain individuals examined.ZusammenfassungAn Patienten mit rekurrenter Aphthae und an Kontrollpersonen wurden Versuche über Lymphozytentransformation durchgeführt unter Verwendung von hitzebehandelten Herpes-Virus-hominis- und Adenovirusantigenen verschiedener Typen.Von diesen Virusantigenen riefen nur diejenigen von Herpes-Virus hominis und von Adenovirus Typ I blastogene Transformation in höherem Maße in den Lymphozytenkulturen aphthöser Patienten hervor, während die Kulturen der Kontrollpersonen unverändert blieben.Bei einigen Adenovirustypen wurde Plasmazellreaktion beobachtet. In den Lymphozytenkulturen bestimmter Versuchspersonen wurde neben Lymphoblasten die Wahrscheinlichkeit von polynuklearen Riesenzellen in Erwägung gezogen.