Kuniya Fujita

Electron microscopic localization of alkaline phosphatase in the trigeminal ganglion of the rat.

SummaryThe electron microscopic demonstration of alkaline phosphatase (ALP) was carried out on the trigeminal ganglion of the rat using the calcium lead modification method by Gomori (Gomori, 1952; Molnar, 1952).The ALP reaction was localized on the junction of capsular cells and nerve cells, in the cytoplasm of some dark capsular cell and in that of the endothelial cell: The enzymatic reaction products (1) existed throughout the entire length of the junction of clear cells and capsular cells, (2) aggregated at some points of the junction of dark cells and capsular cells, (3) existed on the smooth and/or rough surfaced endoplasmic reticulum and on the ribosomes of some dark capsular cells.

The ultrastructural localization of acetylcholinesterase in the trigeminal ganglion of the rat

SynopsisThe presence of acetylcholinesterase in the trigeminal ganglion of the rat has been investigated at the light and electron microscope levels using Karnovsky and Rootss method.The acetylcholinesterase reaction was mainly localized in the neuron. In the nerve cell body, the reaction was observed:(1)as small electron opaque dots corresponding to fixed or free ribosomes;(2)either on the membrane of or within the smooth-surfaced endoplasmic reticulum or both;(3)either on the membrane of or within the large vesicles or both;(4)in the ‘labyrinthine structure’ at the junction of the capsular cells and nerve cell bodies; and(5)on the nuclear envelope. In the nerve fibre, whether myelinated or unmyelinated, the acetylcholinesterase reaction was confined to the axolemma and to the neurotubules.