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Dive into the research topics where Kurt Dörreich is active.

Publication


Featured researches published by Kurt Dörreich.


Journal of Biological Chemistry | 1995

Molecular cloning and characterization of a rhamnogalacturonan acetylesterase from Aspergillus aculeatus. Synergism between rhamnogalacturonan degrading enzymes.

Sakari Kauppinen; Stephan Christgau; Lene Venke Kofod; Torben Halkier; Kurt Dörreich; Henrik Dalbøge

A rhamnogalacturonan acetylesterase (RGAE) was purified to homogeneity from the filamentous fungus Aspergillus aculeatus, and the NH-terminal amino acid sequence was determined. Full-length cDNAs encoding the enzyme were isolated from an A. aculeatus cDNA library using a polymerase chain reaction-generated product as a probe. The 936-base pair rha1 cDNA encodes a 250-residue precursor protein of 26,350 Da, including a 17-amino acid signal peptide. The rha1 cDNA was overexpressed in Aspergillus oryzae, a filamentous fungus that does not possess RGAE activity, and the recombinant enzyme was purified and characterized. Mass spectrometry of the native and recombinant RGAE revealed that the enzymes are heterogeneously glycosylated. In addition, the observed differences in their molecular masses, lectin binding patterns, and monosaccharide compositions indicate that the glycan moieties on the two enzymes are structurally different. The RGAE was shown to act in synergy with rhamnogalacturonase A as well as rhamnogalacturonase B from A. aculeatus in the degradation of apple pectin rhamnogalacturonan. RNA gel blot analyses indicate that the expression of rhamnogalacturonan degrading enzymes by A. aculeatus is regulated at the level of transcription and is subjected to carbon catabolite repression by glucose.


Biochemical Journal | 1996

Pectin methyl esterase from Aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme

Stephan Christgau; Lene Venke Kofod; Torben Halkier; Lene Nonboe Andersen; Maria Hockauf; Kurt Dörreich; Henrik Dalbøge; Sakari Kauppinen


Journal of Biological Chemistry | 1994

Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus.

Lene Venke Kofod; Sakari Kauppinen; Stephan Christgau; L N Andersen; H P Heldt-Hansen; Kurt Dörreich; Henrik Dalbøge


Archive | 1993

A novel enzyme and a dna sequence

Kurt Dörreich; Flemming Mark Christensen; Yvette Schnell; Marcel Mischler; Henrik Dalbøge; Hans Peter Heldt-Hansen


Archive | 1995

Method for production of a fruit or vegetable juice concentrate

Walter Janda; Kurt Dörreich


Archive | 1992

Sg(b)-1,4-galactanase and a dna sequence

Kurt Dörreich; Henrik Dalbøge; Jan Moller Mikkelsen; Marcel Mischler; Flemming Mark Christensen


Archive | 1993

Aspergillus aculeatus rhamnogalacturon acetyl esterases, DNA sequences encoding the enzymes and methods of use thereof

Kurt Dörreich; Flemming Mark Christensen; Yvette Schnell; Marcel Mischler; Henrik Dalb.o slashed.ge; Hans Peter Heldt-Hansen


Archive | 1992

β-1,4-galactanase and a DNA sequence

Kurt Dörreich; Henrik Dalbøge; Jan Moller Mikkelsen; Marcel Mischler; Flemming Mark Christensen


Archive | 1993

Endo-β-1,4-glucanase and a DNA sequence

Kurt Dörreich; Flemming Mark Christensen; Yvette Schnell; Marcel Mischler; Henrik Dalbøge; Hans Peter Heldt-Hansen


Archive | 1993

Ein neuartiges enzym und seine dna sequenz

Flemming Mark Christensen; Henrik Dalbøge; Kurt Dörreich; Hans Peter Heldt-Hansen; Marcel Mischler; Yvette Schnell

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