Kwang Bae Yoon

Complete mitochondrial genome of the Korean ikonnikov’s bat Myotis ikonnikovi (Chiroptera: Vespertilionidae)

Abstract We have characterized the complete mitochondrial genome of a Korean ikonnikov’s bat species, Myotis ikonnikovi. Total length of the mitogenome is 16,585 bp with a total base composition of 34.0% A, 30.4% T, 22.8% C and 12.8% G. The mitogenome consists of 13 protein-coding genes, 2 rRNA (12S and 16S RNA) genes, 22 tRNA genes and 1 control region. The mitogenome of M. ikonnikovi exhibited similar features compared with the previously determined mitogenome of a Myotis species, M. formosus. The results will contribute to our understanding of the mechanisms underlying mitogenome evolution in the genus Myotis bats.

The complete mitochondrial genome of the Ussurian tube-nosed bat Murina ussuriensis (Chiroptera: Vespertilionidae) in Korea

The complete mitochondrial genome of a Korean Ussurian tube-nosed bat, Murina ussuriensis, was determined. The mitochondrial genome of M. ussuriensis is 17,285 bp in length with a total base composition of 33.8% A, 28.9% T, 23.8% C, and 13.4% G. All the protein-coding genes (total length of 11,401 bp) were encoded in H-strand except for ND6 in L-strand. The total length of 22 tRNA genes was 1516 bp varying from 62 bp (tRNA Ser (AGY)) to 74 bp (tRNA Leu (UUR) and tRNA Gln ). The 12S rRNA and 16S rRNA genes were 972 and 1558 bp in length, respectively. The D-loop region was 2496 bp in length.

The complete mitochondrial DNA genome of a greater horseshoe bat subspecies, Rhinolophus ferrumequinum quelpartis (Chiroptera: Rhinolophidae)

There are two subspecies of Rhinolophus ferrumequinum currently recognized in South Korea. The Korean greater horseshoe bat subspecies, Rhinolophus ferrumequinum quelpartis, is distributed only in Jeju Island. The complete mitochondrial genome of the island subspecies was determined and revealed 99.7% similarity to the mainland subspecies Rhinolophus ferrumequinum korai. If d-loop region is excluded, similarity of the two genomes was 99.9%.

The complete mitochondrial genome of the greater horseshoe bat subspecies, Rhinolophus ferrumequinum korai (Chiroptera: Rhinolophidae).

The total length of the mitogenome of Rhinolophus ferrumequinum korai is 16,839 bp with a total base composition of 31.8% A, 25.4% T, 28.7% C, and 14.0% G. The mitogenome consists of 13 protein-coding genes, 2 rRNA (12S and 16S RNA) genes, 22 tRNA genes, and 1 control region.

Complete mitochondrial genome sequence of the Indian pipistrelle Pipistrellus coromandra (Vespertilioninae)

ABSTRACT We characterized the complete mitogenome of Pipistrellus coromandra (Indian pipistrelle) for comparative analysis of mitogenomes and for resolving the phylogenetic relationship of four tribes in the subfamily Vespertilioninae. The mitogenome size of P. coromandra was 17,153 bp, with a control region and a typical set of 37 mitochondrial genes. The nucleotide composition of the P. coromandra mitogenome showed an AT bias with a nucleotide composition of 33.5% A, 30.7% T, 13.3% G, and 22.5% C. The mitochondrial protein-coding genes in P. coromandra use the standard start codon (ATN), two stop codons (TAA and AGA), and two incomplete stop codons (TA- and T--). The intertribal relationship of four tribes was highly resolved from the phylogenetic analysis of mitogenome sequences.

Complete mitochondrial genome of the serotine bat (Eptesicus serotinus) in Korea.

Abstract Serotine bats of Eptesicus serotinus are uncommon species in South Korea due to habit loss and destruction. We describe the characteristics of complete mitochondrial genome and gene organization of E. serotinus in this article. The complete mitogenome of E. serotinus is 16,665 bp long with a total base composition of 32.8% A, 29.8% T, 23.5% C and 13.8% G. The mitogenome consists of 13 protein-coding genes (11,410 bp), 2 rRNA (12S and 16S RNA) genes (2524 bp), 22 tRNA genes (1504 bp) and 1 control region (1225 bp). The results will contribute to understand the genetic structure of Korean E. serotinus.

The complete mitochondrial genome of the jumping spider Telamonia vlijmi (Araneae: Salticidae)

Abstract The complete mitochondrial genome of a jumping spider Telamonia vlijmi was sequenced and its total length is 14,601 bp, with A 35.5%, T 41.8%, C 8.7%, and G 14.0%. Among protein-coding genes, two genes (CO1 and Cytb) start with TTA, uncommon in invertebrate mitogenomes. The standard cloverleaf secondary structure was found in 10 tRNA genes and TV-replacement loop was not found in the other 12 tRNA genes.

The complete mitochondrial genome of the Asian particolored bat Vespertilio sinensis (Chiroptera: Vespertilionidae) in Korea.

Abstract The complete nucleotide sequence of the mitochondrial genome of the Asian particolored bat, Vespertilio sinensis, was determined. The genome organization, gene contents, and codon usage conformed to those of other bat mitochondrial genomes. The total length of the mitogenome of Vespertilio sinensis is 16,971 bp with a total base composition of 32.6% A, 29.6% T, 23.7% C and 14.0% G. The mitogenome consists of 13 protein-coding genes, 2 rRNA (12S and 16S RNA) genes, 22 tRNA genes and 1 control region.

Complete mitochondrial genome of a chipmunk species, Tamias sibiricus (Rodentia: Sciuridae) in Korea

Abstract We sequenced the complete mitochondrial genome (KF668525) of Tamias sibiricus in South Korea. The mitogenome of the Korean chipmunk T. sibiricus was 16,558 bp long with base composition of 33.8% A, 31.1% T, 22.9% C and 12.2% G. Total nucleotide similarity of T. sibiricus and Marmota himalayana (JX069958) genomes was 80.4% ranging from 66.9% (D-loop region) to 97.3% (tRNALeu(CUN)). The present study will contribute to understanding taxonomic status and genetic divergence of Northeast Asian T. sibiricus populations.

Acoustic Species Identification of Korean Myotis Bats (Chiroptera: Vespertilionidae)

Abstract We investigated structure and intensity of 267 echolocation calls that were collected from the five Korean Myotisspecies (M. nettereri, M. petax, M. ikonnikovi, M. macrodactylus and M. formosus). All the Myotis species produced typical FM call pattern with similar echolocation call shapes and outer shapes, producing steep, downward frequency-modu-lated calls. A pulse has two harmonies, which consist of the first harmony with wider bandwidth and the second harmony with narrower bandwidth. The PF of the first harmony is higher than that of the second harmony. The typical FM call structure, with two harmonies and wide bandwidth, might be highly related to fast flying and wide screening in the dense forests. In classification of the echolocation calls by DFA, most of calls from the five species could be well correctly classified. All calls of M. nettereri (100% of 17 calls), M. formosus (95.5% of 22 calls) and M. ikonnikovi (85.7% of 70 calls) could be well discriminated from those of the other species, whereas calls of M. petax and M. macrodactylus could be discriminated by 70.4% of 98 calls and 76.7% of 60 calls, respectively. Our results indicate that the five Korean Myotis species can be well identified by the echolocation calls with high correct classification by DFA.Key Words: Myotis, echolocation call, vespertilionidae, bat, FM call