Kyeong-Suk Kim

Ultrasensitive, label-free detection of cardiac biomarkers with optical SIS sensor

Acute myocardial infarction (MI) is the leading cause of high mortality and morbidity rate worldwide, early and accurate diagnosis can increase the chances of survival. In this work, we report a simple, ultrasensitive, label-free, and high-throughput solution immersed silicon (SIS) immunosensor based on non-reflection condition (NRC) for p-polarized wave for early diagnosis of MI. SIS sensor chips are just a thin dielectric polymer layer on the silicon surface, which can be functionalized for specific application. At NRC, SIS sensors are extremely sensitive to the growing thickness of a bio-layer on the sensor surface while independent of refractive index change of the surrounding medium. Therefore, SIS signal is free from thermal noise, unlike surface plasmon resonance based sensor. Also, there is no need of reference signal which facilitates fast and accurate interaction measurement. Here, SIS technology is applied to tackle two issues in MI diagnosis: high sensitivity with the direct assay and the ability to measure in human serum. Myoglobin, creatine kinase-MB, and cardiac troponin I (cTnI) proteins were used as the MI biomarkers. We were able to measure over a broad concentration range with the detection limit of 5 and 10pg/ml for cTnI in PBS and blood serum, respectively. The response time is about 5min. This novel technique is a suitable candidate for cost effective point-of-care application.

Determination of an Test Condition for IR Thermography to Inspect a Wall-Thinning Defect in Nuclear Piping Components

This study conducted infrared (IR) thermography tests using pipe and plate specimens with artificial wall-thinning defects to find an optimal condition for IR thermography test on the wall-thinned nuclear piping components. In the experiment halogen lamp was used to heat the specimens. The distance between the specimen and the lamp and the intensity of halogen lamp were regarded as experimental parameter. When the distance was set to 1~2 m and the lamp intensity was above 60 % of full power, a single scanning of IR thermography detected all artificial wall-thinning defects, whose minimum dimension was

Label-free detection of hepatitis B virus using solution immersed silicon sensors

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Application Defects Detection in the Small-Bore Pipe Using Infrared Thermography Technique

Highly sensitive solution immersed silicon (SIS) biosensors were developed for detection of hepatitis B virus (HBV) infection in the early stage. The ultrasensitivity for overlayer thickness at the nonreflecting condition for the p-polarized wave is the basis of SIS sensing technology. The change in thickness due to biomolecular interactions and change in refractive index of the surrounding buffer medium were assessed simultaneously using two separate ellipsometric parameters (Ψ and Δ), respectively, from a single sensing spot. A direct antigen-antibody affinity assay was used to detect and quantify hepatitis B surface antigen (HBsAg), which is the early stage biomarker for HBV infection. The detection limit of 10 pg/ml was achieved for HBsAg in the human blood serum, which is comparable with the results of enzyme-linked immunosorbent assay and other hybrid assays. The SIS sensors response time was less than 10 min. The SIS sensors exhibit excellent stability and high signal-to-noise ratio, and are cost-effective, which makes them a suitable candidate for point-of-care applications.

Measurement of defect thickness of the wall thinning defect pipes by lock-in infrared thermography technique

Abstract In the advanced research deducted infrared thermography (IRT) test using 4 inch pipe with artificial wall-thinning defect to measure on the wall-thinned nuclear pipe components. This study conducted for defect detection condition of nuclear small-bore pipe research using deducted condition in the advanced research. Defect process is processed by change for defect length, circumferential direction angle, wall-thinning depth. In the used equipment IR camera and two halogen lamps, whose full power capacitany is 1 kW, halogen lamps and Target pipe experiment performed to the distance of the changed 1 m, 1.5 m, 2 m. To analysis of the experimental results ensure for the temperature distribution data, by this data measure for defect length. artificial defect of 4 inch pipe is high reliabillity in the 2 m, but small-bore pipe is in the 1.5 m from the defect clearly was detected. Keywords: Infrared Thermography, Small-Bore Pipe, Defect Detection, Wall-Thinned Pipe[접수일: 2012. 9. 18, 수정일: 2012. 11. 6, 게재확정일: 2012. 11. 8] *조선대학교 대학원 첨단부품소재공학과, **조선대학교 기계설계공학과, ***전북대학교 기계시스템공학부, Corresponding Author: Department of Mechanical Design Eng., Chosun Univ., 375 Seosuk dong, Dong-Gu, Gwangju 501-759, Korea (E-mail: gsckim@chosun.ac.kr)

Evaluation of Detectable Defect Size for Inner Defect of Pressure Vessel Using Laser Speckle Shearing Interferometry

Mostly piping which is using for the nuclear power plants are made up of carbon steel pipes. The wall thinning defects occurs by the effect of the flow accelerated corrosion of fluid that flows in carbon steel pipes. The defects could be found on the welding part and anywhere in the pipes. The infrared thermography technique which is one of the non-destructive testing method has used for detecting the defects of various kinds of materials over the years. There is a limitation for measuring the defect of metals that have a big coefficient of thermal diffusion. However, a technique using lock-in method gets over the difficulty. Consequently, the lock-in infrared thermography technique has been applied to the various industry fields. In this paper, the defect thickness of the straight pipe which has an artificial defect the inside of the pipes was measured by using the lock-in infrared thermography technique and the result could be utilized in detecting defects of carbon steel pipes.

Near Weld Stress Analysis with Optical and Acoustic Methods

Pressure vessels are used in various industrial fields. If a defect occurs on the inner or outer surface of a pressure vessel, it may cause a massive accident. A defect on the outer surface can be detected by visual inspection. However, a defect on the inner surface is generally impossible to detect with visual inspection. Nondestructive testing can be used to detect this type of defect. Laser speckle shearing interferometry is one nondestructive testing method that can optically detect a defect; its advantages include noncontact, full field, and real time inspection. This study evaluated the detectable size for an internal defect of a pressure vessel. The material of the pressure vessel was ASTM A53 Gr.B. The internal defect was detected when the pressure vessel was loaded by internal pressure controlled by a pneumatic system. The internal pressure was controlled from 0.2 MPa to 0.6 MPa in increments of 0.2 MPa. The results confirmed that an internal defect with a 25 % defect depth could be detected even at 0.2 MPa pressure variation.

Damage Measurement for Molybdenum Thin Film Using Reflection-Type Digital Holography

Residual stress induced by brazing and bead welding have been analyzed based on acousto-elasticity and optical interferometry. Acousto-elasticity is to probe compressive or tensile residual stresses from acoustic velocity measurements and optical interferometry is to measure strain induced by an external tensile load. It has been theorized that a compressive residual stress increases the elastic modulus and that an external tensile stress reduces the increase in the elastic modulus. From the tensile stress that brings back the elastic modulus to the nominal value, the residual stress can be estimated. Compressive residual stresses have been estimated in this fashion for a butt-brazed and a butt-bead-welded steel specimens to be of the order of 5 MPa and 50 MPa respectively.

Method for Measuring Weld Temperature Using an Infrared Thermal Imaging Camera

In the fabrication of electronic circuits used in electronic products, molybdenum thin films are deposited on semiconductors to prevent oxidation. During the deposition, the presence of a particle or dust at the interface between the thin film and substrate causes the decrease of adhesion, performance, and life cycle. In this study, a damage measurement targeting two kinds of glass substrate, with and without particles, was performed in order to measure the change in the molybdenum thin film deposition area in the presence of a particle. Clean and dirty molybdenum thin film specimens were fabricated and directly deposited on a substrate using the sputtering method, and a reflection-type digital holographic interferometer was configured for measuring the damage. Reflection-type digital holography has several advantages; e.g., the configuration of the interferometer is simple, the measurement range can be varied depending on the magnification of a microscopic lens, and the measuring time is short. The results confirm that reflection-type digital holography is useful for the measurement of the damage and defects of thin films.

Measurement of Inner Defects and out of Plane Deformation of Pressure Vessel in Piping of Circulation System Using Shearography

In this paper, a method is tested to measure temperatures in high-temperature welds. Protective glass was installed between an infrared thermal imaging camera and a heat source, and temperature compensation was applied to the measuring instruments. When the temperature of halogen lamps was taken in real-time and measured by the thermal camera, the temperature was found to be almost invariant with the distance between the camera and heat source. The temperature range could be predicted, through correlations with the thickness of the protective glass and the measured distance. This study suggests that the temperature measurement of welds obtained by using an infrared thermal imaging camera is valid, through experimental testing of heat sources.