Kyu-Hwan Choi

Study on characteristic of mycelial culture in ear mushroom

ABSTRACT : This study was performed to obtain the scientific data an artificial culture of ear mushroom( Auricularia auricula ). Liquid cultural of Auricularia auricula . mycelium was characterized with respect to temperature, pH, compositions of inorganic, carbon and nitrogen. The its optimum temperature and media pH were at 25℃ and pH 5.0 ~ 6.0 on the mycelial growth, respectively. The optimum inorganic compositions and concentration for mycelial growth were MgSO 4 0.05%(w/v) and KH 2 PO 4 0.1%(w/v), respectively. The optimum carbon sources and nitrogen sources were black-sugar 2%(w/v) and soybean powder at 0.3~0.5%(w/v) with7 days optimum culture time.KEYWORDS : Auricularia auricula., carbon sources, nitrogen sources * Corresponding author 서 론 목이는 세계적으로 널리 분포되어 있는 버섯으로 한국, 중국, 일본 등지에서 많이 발생되고 있는 버섯이다(Chen, Hou. 1979). 목이에 대한 분류 체계는 목이목(Auriculariales) 목이과(Auricu-lariaceae) 목이속( Auricularia )에 속하는 목이( Auricularia au-ricula )와 털목이버섯( Auricularia polytriha

Bag cultivation of Pleurotus ostreatus with Miscanths species substrates

In analysis of inorganic components of Miscanthus sinensis Andersson var. sinensist, Phosphate increased with the progress of growing stage. in other way potassium, calcium and magnesium was inclined to decrease. The total nitrogen content in the Miscanthus sinensis Andersson var. sinensist was increased until second age cultivated stage, but in its third year, it was decreased apparently. In other way the total carbon content of Miscanthus sinensis was not changed until second year cultivation. But rapid increasing was happen in its third year cultivation stage. 3 year cultivated Miscanthus sinensis Andersson var. sinensist which has a plentiful carbon source can be used as a good culture media source for the formation of mushroom`s fruit body. When rice bran added at the amount of 20% to the keep in 65% moisture Miscanthus sinensis Andersson var. sinensist, the fermentation of culture material was well done and the temperature of fittest for thermophilic microorganism growth was maintained for 5 days from 5 days after treatment. Rice bran 20% added to the Miscanthus sinensis Andersson var. sinensist was the fittest fermentation culture media for the Pleurotus ostreatus vinyl-bag type cultivation. When Pleurotus ostreatus was cultivated in this culture media, the yield was increased by 60% than whole Miscanthus sinensis Andersson var. sinensist containing culture media. The fermentation Miscanthus sinensis Andersson var. sinensist culture media could be substituted by 20% the sawdust in existing culture media(sawdust 50%+cottonseed bark 30%+cottonseed meal 20%).

The physicochemical properties of kenaf(Hibiscus cannabinus L.) as mushroom culture media source

To investigate the usefulness of Kenaf(Hibiscus cannabinus L.) as mushroom culture media source, we analyzephysical condition and contents of nutritional components. The water absorption rate of Kenaf bast was 578% and it was 95%higher than that of poplar sawdust’s. This was caused by Kenaf’s porous cellular structure. so it could give more moisture andoxygen to cultured mushroom. Total carbon contents of Kenaf was 91.4%, it was quite higher than that of poplar sawdust, wheatbran and rice bran. Total nitrogen content was 1.76% and C/N ratio was 51.9. The content of NFE(Nitrogen free extract) was46.6% and it was similar with rice bran. Cellulose content was higher than poplar but lignin content was lower. speciallyhemicellulose and pectin complex which more digestible carbon source to mushroom was 3.7% higher than poplar. Mineralcomponent and amino acid contents were also maintained high compared with poplar. Fe was 4.2 times, P 3.2 times, K 2.2times more and Ca was higher 16 mg/kg than poplar. The content of amino acid was quite more higher than poplar sawdust butlower than chaff. Consequently Kenaf had a good trait for basic support material in mushroom culture media and also had agood character as nutritional source.

Condition of the most suitable inoculation and manufacture of spawn of ear mushroom

Studies were made to optimize the media composition and cultural condition for mycelial growth of ear mushroom(Auricularia auricula). Sawdust spawn of media composition for optimal growth were found to be quercus sawdust combination of 20% rice bran and poplar sawdust combination of 10% wheat bran were the best of the optimal combination. The optimal condition of the most suitable inoculation amount showed that quercus spawn and poplar spawn were 15g, 25g, respectively.