Kyung-Hwan Moon

Geographical Shift in Blooming Date of Kiwifruits in Jeju Island by Global Warming

A kiwifruit cultivar `Hayward` has been grown in Jeju Island where the current climate is suitable for growth and development of this crop. Prediction of the geographical shift in the phenology can help the kiwifruits growers to adapt to the local climate change in the future. Two phenology models (i.e., chill-day and DVS) were parameterized to estimate flowering date of kiwifruits `Hayward` based on the data collected from field plots and chamber experiments in the southern coastal and island locations in South Korea. Spatio-temporally independent datasets were used to evaluate performance of the two models in predicting flowering date of `Hayward`. Chill-day model showed better performance than DVS model (2.5 vs. 4.0 days in RMSE). Daily temperature data interpolated at a higher spatial resolution over Jeju Island were used to predict flowering dates of `Hayward` in 2021-2100 under the A1B scenario. According to the model calculation under the future climate condition, the flowering of kiwifruits shall accelerate and the area with poor flowering might increase due to the warmer winter induced insufficient chilling. Optimal land area for growing `Hayward` could increase for a while in the near future (2021-2030), whereas such areas could decrease to one half of the current areas by 2100. The geographic locations suitable for `Hayward` cultivation would migrate from the current coastal area to the elevated mountain area by 250 m.

Soil Dehydrogenase Activity and Microbial Biomass C in Croplands of JeJu Province

This study was carried out to evaluate the soil dehydrogenase activity and microbial biomass C with soil type and land use in cropland of JeJu region. Soil chemical properties, dehydrogenase activity, and microbial biomass C were analyzed after sampling from upland (50 sites), orchard (50 sites), paddy (30 sites), horticultural facility (30 sites) in March. Average pH values was at 6.3 in upland soil, however soil chemical properties showed a large spatial variations in both orchard and horticultural facility soil. The Zn and Cu contents increased by the continuous application of pig manure compost in some citrus orchard soil. Soil dehydrogenase activity and microbial biomass C were higher in non-volcanic ash than in volcanic ash soil regardless of land use type. Soil dehydrogenase activity was two to four times higher in upland than in the others. It was at 38.7 ug TPF in non-volcanic ash of upland soil. Microbial biomass C content was very high in horticultural facility soil and it showed at 216.8 in non-volcanic ash. Soil dehydrogenase activity showed a positive correlation with organic matter (=0.59), Zn (=0.65), and Cu (=0.66) in non-volcanic ash horticultural facility soil. There was a negative correlation (=0.57) between soil organic matter and dehydrogenase activity in volcanic ash upland soil.

Effect of Temperature Condition on Nitrogen Mineralization of Organic Matter and Soil Microbial Community Structure in non-Volcanic Ash Soil

This study was carried out to evaluate effect of temperature condition on nitrogen mineralization of organic matter, distribution of microbial group by PLFA profiles, and soil microbial community in non-volcanic ash soil. Dried soil 30 g mixed well each 2 g of pellet (OFPE) organic fertilizers, pig manure compost (PMC), and food waste compost (FWC). And then had incubated at , , and , respectively. Nitrogen mineralization rate increased with increasing temperature and that was in the order of FWC>OFPE>PMC. Distribution ratio of microbial group by PLFA profiles showed that was different significantly according to incubation temperature and the type of organic matter. As incubating time passed, density of microbial group decreased gradually. The Gram-bacteria PLFA/Gram+ bacteria PLFA, Fungi PLFA/Bacteria PLFA, and Unsaturated PLFA/saturated PLFA ratios were decreased according to the increasing temperature gradually. Principal component analysis using PLFA profiles showed that microbial community structures were composed differently by temperature factor at both 75 days () and 270 days (). In conclusion, Soil microbial community structure showed relative sensitivity and seasonal changes as affected by temperature and organic matter type.

Estimation of Duration of Low-temperature in Winter Season Using Minimum Air Temperature on January

The duration of low temperature in winter season is one of the important agrometeorological characteristics in crop growing fields. This study was conducted to develop a method to estimate the duration of low-temperature with monthly meteorological data. Using daily meteorological data from 61 observation sites from 1981 to 2010, we analyzed the relationships between the averages of monthly temperature minima and the durations of low-temperature ranging from -15 to , The monthly mean of the January minimum air temperature was appropriate for theestimation of the durations of lowtemperature below . We tested a simple second order equation to predict durations of low-temperature. To apply the equation to various temperature ranges, we suggested two different equations for the estimation of coefficients a and b, which are dependent on the base temperatures from -15 to . Thevalidation of the equations using other daily meteorological datasets from 1971 to 2000 showed that they were appropriate for the range from -10 to , but underestimated at .

Taxonomical Classification and Genesis of Donggui Series in Jeju Island

This study was conducted to reclassify Donggui series based on the second edition of Soil Taxonomy and to discuss the formation of Donggui series in Jeju Island. Morphological properties of typifying pedon of Donggui series were investigated and physico-chemical properties were analyzed according to Soil survey laboratory methods manual. The typifying pedon has very dark grayish brown (10YR 3/2) silt loam A horizon (0~17 cm), gravelly very dark grayish brown (10YR 3/2) silt loam BA horizon (17~42 cm), gravelly very dark grayish brown (10YR 3/2) silty clay loam Bt1 horizon (43~80 cm), brown (7.5YR 4/6) silty clay Bt2 horizon (80~105 cm), and brown (10YR 5/4) silty clay Bt3 horizon (105~150 cm). It is developed in lava plain and are derived from basalt and pyroclastic materials. The typifying pedon contains 1.3~2.1% oxalate extractable (Al + 1/2 Fe), less than 85% phosphate retention, and higher bulk density than 0.90 . That can not be classified as Andisol. But it has an argillic horizon from a depth of 22 to 150 cm and a base saturation (sum of cations) of less than 35% at 125 cm below the upper boundary of the argillic horizon. That can be classified as Ultisol, not as Andisol and Inceptisol. It has udic soil moisture regime, and can be classified as Udalf. Also that meets the requirements of Typic Hapludalf. It has 18-35% clay at the particle-size control section, and have thermic soil temperature regime. Therefore Donggui series can be classified as fine loamy, mixed, thermic family of Typic Hapludalfs, not as fine silty, mixed, thermic family of Dystric Eutrudepts.

Validation of quantitative real-time PCR reference genes for the determination of seasonal and labor-specific gene expression profiles in the head of Western honey bee, Apis mellifera

Honey bee is not only considered an important pollinator in agriculture, but is also widely used as a model insect in biological sciences, thanks to its highly evolved sociality, specialization of labor division, and flexibility of colony management. For an intensive investigation of the seasonal and labor-dependent expression patterns of its genes, accurate quantification of the target gene transcription level is a fundamental step. To date, quantitative real-time PCR (qRT-PCR) has been widely used for rapid quantification of gene transcripts, with reliable reference gene(s) for normalization. To this end, in an attempt to search for reliable reference genes, the amplification efficiencies of six candidate reference genes (rp49, rpL32, rpS18, tbp, tub, and gapdh) were determined. Subsequently, four genes (rpL32, rpS18, tbp, and gapdh) with PCR efficiencies of 90% to 110% were evaluated for their expression stabilities with three programs (geNorm, NormFinder, and BestKeeper) and used for normalization of seasonal expression patterns of target genes in the forager and nurse heads. Although the three programs revealed slightly different results, two genes, rpS18 and gapdh, were suggested to be the optimal reference genes for qRT-PCR-based determination of seasonal and labor-specific gene expression profiles. Furthermore, the combined use of these two genes yielded a more accurate normalization, compared with the use of a single gene in the head of honey bee. The validated reference genes can be widely used for quantification of target gene expression in honey bee head although it is still remained to be elucidated the expression levels of the selected reference genes in specific tissues in head.

Effect of Consecutive Application of Organic Matter on Soil Chemical Properties and Enzyme Activity in Potato Cultivation Soil

This study was carried out to evaluate effect of consecutive application of organic matter on soil chemical properties and dehydrogenase, acid phosphatase activity in non-volcanic ash soil during three cropping season. Organic matter mixture and organic fertilizer (MOF, ), food waste compost (FWC, ), and pig manure compost (PMC, 2,000, 4,000, and ) were applied for each cropping season. Soil pH values were increased after three cropping season in all treatment. In the soils of the increased application of PMC, soil pH, total-nitrogen, available phosphate, exchangeable cations (K, Ca, and Mg), and heavy metal (Zn and Cu) contents were increased. In addition, Soil dehydrogenase activity was significantly increased in proportions to PMC application rate and cropping season during potato cultivation period. The activity was two times higher in PMC () than control after the third cropping season. Soil dehydrogenase activity was in order of PMC>FWC>NPK+PMC>MOF. Acid phosphatase activity was higher in PMC () than other treatment. Soil Zn content and dehydrogenase activity showed linearly correlation, which were MOF (=0.427), FWC (=0.427) and PMC (=0.411, p