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Featured researches published by Kyung Tae Chung.


Toxicology in Vitro | 2009

Naringenin-induced apoptosis is attenuated by Bcl-2 but restored by the small molecule Bcl-2 inhibitor, HA 14-1, in human leukemia U937 cells

Cheng-Yun Jin; Cheol Keun Park; Jun-Hyuk Lee; Kyung Tae Chung; Taeg Kyu Kwon; Gi-Young Kim; Byung Tae Choi; Yung Hyun Choi

Naringenin, a naturally occurring citrus flavonone, has shown cytotoxicity in various human cancer cell lines as well as inhibitory effects on tumor growth and there is increasing interest in its therapeutic applications. In this study, the effect of ectopic Bcl-2 expression on naringenin-induced apoptosis was investigated. We found that Bcl-2 overexpression markedly protected human leukemia U937 cells from time- and dose-dependent induction of apoptosis by naringenin, as did caspase-3 and caspase-9 inhibitors. Additionally, Bcl-2 overexpression attenuated naringenin-induced Bax translocation and cytosolic release of cytochrome c. Our results also indicated that co-administration of HA14-1 and naringenin increased apoptosis in Bcl-2 overexpressing U937 cells by restoring mitochondrial dysfunction and activation of caspase-9 and caspase-3, as well as by cleavage of poly (ADP-ribose) polymerase. Taken together, these observations indicate that Bcl-2 confers apoptosis resistance to naringenin by inhibiting a mitochondrial amplification step in U937 cells.


Biotechnology and Bioprocess Engineering | 2003

Isolation of proteins that speifically interact with the ATPase domain of mammalian ER chaperone, BiP

Kyung Tae Chung; Tae Ho Lee; Gyong Suk Kang

BiP, immunoglobulin binding protein, is an ER homologue of Hsp 70. However, unlike other Hsp70 proteins, regulatory protein(s) for BiP has not been identified. Here, we demonstrated the presence of potential regulatory proteins for BiP using a pull-down assay. Since BiP can bind any unfolded protein, only the ATPase domain of BiP was used for the pull-down assay in order to minimize nonspecific binding. The ATPase domain was cloned to produce recombinant protein, which was then conjugated to CNBr-activated agarose. The structural conformation and ATP hydrolysis activity of the recombinant ATPase domain were similar to those of the native protein. Eight proteins from metabolically labeled mouse plasmacytoma cells specifically bound to the recombinant ATPase protein. The binding of these proteins was inhibited by excess amounts of free ATPase protein, and was dependent on the presence of ATP. These proteins were eluted by ADP. Of these proteins, Grp 170 and BiP where identified, while the others were not identified as known ER proteins, from Western blot analyses. The presence of the ATPase-binding proteins for Bip was first demonstrated in this study, and our data suggest similar regulatory machinery for BiP may exist in the ER, as found in prokaryotes and other cellular compartments.


Journal of Life Science | 2007

Lipid A of Salmenella typhimurium Suppressed T-cell Mitogen-Induced Proliferation of Murine spleen Cells in the Presence of Macrophage

Gyong Suk Kang; Kyung Tae Chung

Infection with virulent or attenuated Salmonella typhimuriumhas known to induce reduction in proliferative responses of spleen cells. We investigated a role of lipid A from S. typhimurium, a B cell mitogen, on proliferation of spleen cells by T cell mitogens such as concanavaline A and phytohemagglutinin under in vitro and ex vivo conditions. Lipid A alone induced proliferation of spleen cells in vitroin a dose-dependent manner. However, subsequent treatment of concanavaline A or phytohemagglutin in after lipid A treatment induced proliferation suppression of murine spleen cells in vitro and ex vivo. Removal of macrophages from spleen cells, which were obtained from a lipid A-injected mouse, restored proliferation by concanavaline A and phytohemagglutinin, indicating that macrophages appeared to play a role in lipid A-induced suppression. Secreted molecules from macrophages did not accounted for the suppression because suppressive effect was not achieved when the supernatant from macrophage-containing spleen cell culture was conditioned to macrophage-depleted spleen cell culture. Co-culture of spleen cells from lipid A-treated and ?untreated mice showed proliferation suppression as increasing cell numbers of lipid A-treated mouse. These data suggested that the cell-to-cell contact of macrophage with splenic lymphocyte cells is responsible for immune responses against lipid A, which is applicable to the case of human S. typhi infection.


International Immunopharmacology | 2005

Effect of water-soluble proteoglycan isolated from Agaricus blazei on the maturation of murine bone marrow-derived dendritic cells.

Gi-Young Kim; Moo-Yeol Lee; Hee-Jeong Lee; Dong-Oh Moon; Chang-Min Lee; Cheng-Yun Jin; Yung Hyun Choi; Yong-Kee Jeong; Kyung Tae Chung; Jae-Yun Lee; Inhak Choi; Yeong-Min Park


한국생명과학회 심포지움 | 2008

Effect of Water Extract of Alliun sativum L. on the Recovery of Exercise-induced Fatigue

Yong Tae Lee; Yung Hyun Choi; Dong Il Park; Byung Tae Choi; Ho-Ok Park; Seok Beom Roh; Kyung Tae Chung


Archive | 2008

Effect of Garlic Extract on the Activation Pattern of MAPK Signaling in the Rat Heart After a Bout Exercise

Jun Hyuk Lee; Kyung Tae Chung; Yong Tae Lee; Yung Hyun Choi; Byung Tae Choi


한국생명과학회 심포지움 | 2006

Study on the Characterization and immunostimulatory activities of novel proteoglycan extracted from Phellinus linteus

Tae-Yeon Kim; Gi-Young Kim; Kyung Tae Chung; Byung Tae Choi; Yeong-Min Park; Yung Hyun Choi; Yong-Kee Jeong


한국생명과학회 심포지움 | 2006

Production of Mannose-Binding Protein cDNA from Rat Liver and Recombinant Protein using a Bacterial Expression System

Kyung Tae Chung; Ju-Hong Kim; YoungHee Kim; Hyun-Mi Kwon


한국생명과학회 심포지움 | 2006

Development of a Chimera Molecule, GFP-ATF6 to monitor Stress-Condition of Cells in vitro

Ju Hong Kim; Yong-Kee Jeong; Kyung Tae Chung


한국생명과학회 심포지움 | 2006

Change of Organic Acid Amounts in Phellinus mycelia-fermented Alcoholic Beverage over Two-Month Period

Kyung Tae Chung; Yun Yeong Lee; Byung Tae Choi; Hak-Seob Lim; Yong-Kee Jeong; Yong Hee Lee

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Byung Tae Choi

Pusan National University

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Gi-Young Kim

Jeju National University

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Yeong-Min Park

Pusan National University

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Yung Hyun Choi

UPRRP College of Natural Sciences

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