Kyung Yun Lee
Biotechnology Institute
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Microbial Cell Factories | 2010
Kyung Yun Lee; Jong Myoung Park; Tae Yong Kim; Hongseok Yun; Sang Yup Lee
BackgroundZymomonas mobilis ZM4 is a Gram-negative bacterium that can efficiently produce ethanol from various carbon substrates, including glucose, fructose, and sucrose, via the Entner-Doudoroff pathway. However, systems metabolic engineering is required to further enhance its metabolic performance for industrial application. As an important step towards this goal, the genome-scale metabolic model of Z. mobilis is required to systematically analyze in silico the metabolic characteristics of this bacterium under a wide range of genotypic and environmental conditions.ResultsThe genome-scale metabolic model of Z. mobilis ZM4, ZmoMBEL601, was reconstructed based on its annotated genes, literature, physiological and biochemical databases. The metabolic model comprises 579 metabolites and 601 metabolic reactions (571 biochemical conversion and 30 transport reactions), built upon extensive search of existing knowledge. Physiological features of Z. mobilis were then examined using constraints-based flux analysis in detail as follows. First, the physiological changes of Z. mobilis as it shifts from anaerobic to aerobic environments (i.e. aerobic shift) were investigated. Then the intensities of flux-sum, which is the cluster of either all ingoing or outgoing fluxes through a metabolite, and the maximum in silico yields of ethanol for Z. mobilis and Escherichia coli were compared and analyzed. Furthermore, the substrate utilization range of Z. mobilis was expanded to include pentose sugar metabolism by introducing metabolic pathways to allow Z. mobilis to utilize pentose sugars. Finally, double gene knock-out simulations were performed to design a strategy for efficiently producing succinic acid as another example of application of the genome-scale metabolic model of Z. mobilis.ConclusionThe genome-scale metabolic model reconstructed in this study was able to successfully represent the metabolic characteristics of Z. mobilis under various conditions as validated by experiments and literature information. This reconstructed metabolic model will allow better understanding of Z. mobilis metabolism and consequently designing metabolic engineering strategies for various biotechnological applications.
asia-pacific bioinformatics conference | 2011
Jong Myoung Park; Kyung Yun Lee; Tae Yong Kim; Sang Yup Lee
Annual Fall Meeting of KIChE 2011 | 2011
Kyung Yun Lee; H.U Kim; T.Y Kim; SangYup Lee
Annual Fall Meeting of KIChE 2011 | 2011
Kyung Yun Lee; Ys Jang; M.J. Han; J Lee; SangYup Lee
Annual Fall Meeting of KIChE 2011 | 2011
Kyung Yun Lee; Ys Jang; Xiao-Xia Xia; Zhi-Gang Qian; SangYup Lee
2011 The Korean Society for Biotechnology and Bioengineering Fall Meeting & International Symposium | 2011
Jin Eyun Kim; Kyung Yun Lee; Jong Myoung Park; Tae Yong Kim; Hongseok Yun; Sang Yup Lee
2011 The 24th International Symposium on Chemical Engineering (24th ISChE) | 2011
Yu Bin Kim; Kyung Yun Lee; Jong Myoung Park; Tae Yong Kim; Hongseok Yun; Sang Yup Lee
2011 The 22nd International Conference on Genome Informatics | 2011
Kyung Yun Lee; Jong Myoung Park; Tae Yong Kim; Sang Yup Lee
2011 The 22nd International Conference on Genome Informatics | 2011
Kyung Yun Lee; Seok Hyun Park; Tae Yong Kim; Sang Yup Lee
2011 International Meeting of the Microbiological Society of Korea | 2011
Kyung Yun Lee; Jong Myoung Park; Hongseok Yun; Sang Yup Lee