Kyunghoon Kim

Unidirectional transfer of broad host-range plasmid R1162 during conjugative mobilization. Evidence for genetically distinct events at oriT.

A segment of R1162 DNA containing genes for conjugative mobilization (Mob) and the origin of transfer (oriT) was integrated into the Escherichia coli chromosome. Bacterial genes were transferred in a polar fashion during conjugative mobilization of the integrated segment by a self-transmissible plasmid vector. The direction of transfer, together with the properties of mutated derivatives of oriT, indicate that initial cleavage of oriT, and subsequent religation after transfer, entail different mechanisms that can be distinguished genetically.

Enhancement of Skin Antioxidant and Anti-Inflammatory Potentials of Agastache rugosa Leaf Extract by Probiotic Bacterial Fermentation in Human Epidermal Keratinocytes

Hye-Won Lim1†, Yoonjin Lee2†, Yu-Hua Huang1, Ji-Young Yoon3, Su Hee Lee4, Kyunghoon Kim4*, and Chang-Jin Lim1,5* 1Shebah Biotech Inc., G-Tech Village, Chuncheon 24398, Republic of Korea 2R&D Center, Cosmocos Corporation, Incheon 21698, Republic of Korea 3Jeonju AgroBio-Materials Institute, Joenju 54810, Republic of Korea 4Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea 5Department of Biochemistry, Kangwon National University, Chuncheon 24341, Republic of Korea