L.M. Ferrer

Lack of a specific immune response against a recombinant capsid protein of Jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis

Enzootic nasal tumour (ENT) and sheep pulmonary adenomatosis (SPA) are two contagious adenocarcinomas of the respiratory tract of sheep and goats. Both diseases are associated with related, but distinct, type-D-retroviruses (ENTV and JSRV respectively). No evidence of circulating antibodies has been described in animals affected by either ENT or SPA using antigens from natural sources. We evaluated the usefulness of a recombinant JSRV capsid protein (JSRV-CA) as antigen to study the antibody responses of animals naturally affected by ENT or SPA, using immunoblotting. Positive reactions were detected in the sera of both affected and unaffected sheep and goats. The reactivity was abolished completely by absorption with the GST fusion partner but not by JSRV-CA, suggesting that it was not specific. The results support prior observations indicating that sheep and goats infected by JSRV and ENTV do not develop specific humoral responses to these retroviruses.

Management and environmental factors related to benzimidazole resistance in sheep nematodes in Northeast Spain

A survey to determine the level of parasite resistance to benzimidazoles (BZ) under field conditions was performed on 107 commercial sheep farms located in the Aragon region of northeast Spain. Resistance was measured using the discriminant dose, a simplified form of the in vitro egg hatch assay (EHA). Taking into account the spatial structure of the data, a multivariate approach was applied to management and environmental variables as well as to their relationships with BZ resistance levels compiled from each flock. Results estimated that 11% of flocks had resistant parasite populations, although we suspected the presence of BZ-resistant parasite strains in 98% of the sample. Resistance levels were more similar among the nearest flocks, suggesting a contagious spatial distribution of resistance (i.e., resistance at neighbouring farms was not independent from one another). Management variables such as frequency of deworming, grazing in private pastures and underdosing were positively related to resistance levels, whereas only the use of BZ was negatively related to resistance levels, likely because BZ were replaced by other anthelmintics in flocks where reduced BZ efficacy was suspected. In addition to climatic conditions and seasonality, land use was an environmental variable associated with observed BZ resistance levels. Generally, resistance was highest in cooler and wetter areas but was lower in flocks sampled during January-March compared to flocks sampled in April-June or October-December. Variation partitioning procedures showed that the variation of resistance explained by the effect of environmental variables was higher than management variables. The effects of both variable groups, however, highly overlapped with the spatial structure of resistant levels, which suggests that a considerable amount of the effects attributable to both variable groups may be actually due to the spatial distribution of resistance. The resistance variation explained by the spatial component suggested that other uncontrolled factors acting at short spatial scale (e.g., common management and environmental variables; the importation of resistant strains and their posterior spread across neighbouring flocks; the selection history of the worms carried out by historical management events previous to this survey; and genetic, physiological or both types of parasite population variation) could yield this contagious spatial structure of BZ resistance. Although further research is needed, both seasonal variation and the dependence of resistance levels among neighbouring flocks should be taken into account in the design of future research or observational resistance programmes to minimise spatial and temporal pseudo-replication. Thus, research would avoid biased estimations of resistance prevalence or of its relationship with putative factors.

Infection of rabbit mammary glands with ovine mastitis bacterial strains

An experimental model was developed in rabbits to study ovine mastitis. A total of 19 ovine mastitis bacterial strains (seven Staphylococcus aureus, four Staph. chromogenes, four Staph. hyicus and four Escherichia coli) were used for mammary gland infections. The histopathological results showed that the ovine mastitis types corresponded to experimental infections produced in the rabbit with the ovine strains. These results helped the grading of the bacterial species tested according to the severity of their effects on the mammary gland. The most pathogenic species was Staph. aureus, followed by E. coli, Staph. hyicus and Staph. chromogenes, in that order. There was, however, variation among strains within a given species (e.g. one out of seven Staph. aureus strains gave rise to a mild infection in sheep and rabbits). The procedure was simple and consisted of introducing bacterial suspensions through alternate teat ducts of does with the help of a cannula. It helped minimize the number of animals required in the experiments.

Possible adverse reactions in sheep after vaccination with inactivated BTV vaccines.

PREVENTION programmes against bluetongue virus (BTV) infection have been conducted in numerous countries throughout Europe. In spite of some variations, depending on the expected serotypes of BTV and country-specific conditions, generally, inactivated vaccines have been selected. In the period from

Efficacy of orally administered ivermectin against larval stages of Oestrus ovis in sheep

The efficacy of ivermectin administered orally at a dosage rate of 0.2 mg/kg liveweight against naturally acquired larval infestations of Oestrus ovis in sheep was 100% in a field trial. Ten sheep were free from infestation by first, second and third instar larvae of O. ovis 12 days post treatment, whereas 10 control sheep harboured 37.7 larvae on average, most of them first stage.

Natural border disease virus infection in feedlot lambs

Border disease is caused by border disease virus (BDV, a pestivirus from the family Flaviviridae ) infection in sheep and goats (Vantsis and others 1976). BDV infection causes sizeable economic losses in sheep production around the world. In Spain, serological surveys have found 100 per cent flocks and 8–93 per cent sheep seropositive, respectively (Valdazo-Gonzalez and others 2006). BD is considered a congenital disease, but infections in healthy animals at all age groups may also occur. These are named acute infections and are characterised by transitory leucopaenia and fever associated with viraemia (Nettleton and others 1998). The aim of this study was to evaluate the impact of these acute infections in lambs in a commercial feedlot. A longitudinal observational study in a feedlot located in Aragon (Spain) was carried out. Lambs were supplied to the feedlot at minimum 10 kg bodyweight (BW) (45 days old), housed in groups of 250–300 per pen and slaughtered at 25–35 kg BW (about 45 days later). Thirty-six male lambs were randomly selected, ear-tagged, weighed, clinically evaluated and sampled on days: 0 (day lambs entered the feedlot), 14, 27 and 41. From each lamb, whole blood in anticoagulant solution and serum samples for haematological, virological and serological studies were taken. Haematological analysis with an electronic counter …

Variability of the egg hatch assay to survey benzimidazole resistance in nematodes of small ruminants under field conditions

The egg hatch assay (EHA) is one of the main in vitro methods for detection of benzimidazole resistance in nematode parasites of small ruminants. However, although the EHA has been standardised at the laboratory level, the diagnostic performance of this method has not been fully characterised for field screenings. In the present work, monthly variation of benzimidazole resistance estimated by EHA was surveyed over two years in three sheep flocks and in one goat and an additional sheep flock sharing the same pastures. Resistance was measured by calculating both the effective dose of thiabendazole (TBZ) that inhibited hatching of ≥50% of parasite eggs (ED₅₀) and the proportion (Pdd) of eggs hatching at a discriminating dose of 0.1 μg/ml TBZ. Pdd exhibited higher variability than ED₅₀, in agreement with the higher sensitivity of Pdd to changes in resistance levels. Both resistance parameters, however, were highly correlated, and their variation was similarly related to the same factors. Resistance levels differed among sheep flocks, and the resistance level of the goat flock was higher than that measured for the sheep flock sharing the same pasture. Moreover, monthly variation of resistance in goats did not mirror that recorded in sheep. Resistance levels varied seasonally, with the highest values recorded in the spring, and they were inversely related to the number of days that samples were stored under anaerobic conditions. In addition, they were directly associated with the relative abundance of Teladorsagia spp. but inversely related to the relative abundance of Haemonchus spp. After controlling for the effects of these identified factors for variation, inter-monthly sampling variation due to unknown factors was the main source of variability, accounting for more than 60-70% of variance for both resistance parameters and yielding absolute estimation errors higher than 0.06 for ED₅₀ or 0.2 for Pdd when resistance was estimated from a single sampling. Optimum sample size, estimated from variance components, suggested that at least 4-5 samplings would be needed to halve this absolute error, whereas additional samplings would slightly increase precision but at the cost of substantially increasing sampling effort. More research is needed to identify the main factors involved in this inter-sampling variation to standardise the implementation of EHA under field conditions.

Chronic Proliferative Rhinitis associated with Salmonella enterica subspecies diarizonae serovar 61:k:1, 5, (7) in Sheep in Spain

A chronic proliferative rhinitis in sheep associated with Salmonella enterica subspecies diarizonae serovar 61:k:1, 5, (7) is described. Ten adult sheep from eight traditionally managed Rasa Aragonesa flocks located in Aragon (Spain) were studied. Clinical signs began with bilateral thick mucus nasal discharge, wheezing and snoring and progressed to partial or complete obstruction of the nostrils. Necropsy examination revealed swollen ventral nasal turbinates with a roughened mucosal surface partially covered by small polyps. Histopathology revealed chronic proliferative rhinitis with a predominant population of neutrophils that infiltrated the mucosal epithelium. Plasma cells and macrophages were present in the lamina propria. Organisms expressing Salmonella antigen immunohistochemically were detected within epithelial cells. Salmonella enterica subspecies diarizonae serovar 61:k:1, 5, (7) was isolated as a sole microorganism from nasal swabs taken from five animals. The implication of finding this bacterium in various diseases in sheep and its role as a potential zoonosis are discussed.

A case of ovine female XY sex reversal syndrome not related to anomalies in the sex-determining region Y (SRY).

The human XY gonadal sex reversal is a rare disease. Primary amenorrhea, lack of secondary sexual characteristics and the possible existence of streak gonads are the most common signs of this syndrome. Five cases of this syndrome have been described in the last 42 years in bovines. However, to the knowledge of the authors, no similar descriptions are available in sheep. In the present report, we are presenting the case of a 4 year old ewe 2n = 54,XY showing primary sterility and total absence of masculinization signs. The promoter and the complete coding segment of the sex determining region Y (SRY) were sequenced and found to be absolutely normal. The presence of the testis-specific protein gene (TSPY) was also verified by means of PCR. Necropsy findings include short vagina, lack of uterus and complete absence of masculine remains. Streak gonads concordant with previous descriptions in humans were also found.

Tetralogy of Fallot in a three-month-old lamb: clinical, ultrasonographic and laboratory findings

TETRALOGY of Fallot (TOF) was first described by Niels Stenson in 1671. The prevalence in human beings is 0.28 per 1000 live births, and males and females are affected equally. TOF is a rare complex congenital cardiac defect characterised by a ventricular septal defect (VSD) with an overriding aorta and pulmonic stenosis that develop a compensatory right ventricular hypertrophy. The most common cardiac anomalies in ruminants include VSDs, persistent ductus arteriosus, aortic or pulmonic stenosis, and dysplasia of the atrioventricular valves (Dennis and Leipold 1968, Gopal and others 1986, Maxie and Robinson 2007). In contrast to other cardiac defects, TOF is almost always a lethal condition in farm animals. This short communication describes a case of TOF in a lamb, describing the two-dimensional characteristics using echocardiography and the flow pattern with Doppler. A three-month-old male Rasa Aragonesa lamb was referred to the Veterinary Faculty Ruminant Medical Service at the University of Zaragoza, Spain, with a history of respiratory distress (45 bpm). On physical examination, dyspnoea, exercise intolerance and cyanosis of all the mucous membranes were found. On thoracic auscultation, a grade 5 out of 6 holosystolic murmur was heard at the left basilar region of …