L. Pugliese

Aerobic Fitness Affects the Exercise Performance Responses to Nitrate Supplementation.

PURPOSE Dietary nitrate supplementation has been shown to reduce O2 cost of submaximal exercise, improve exercise tolerance, and enhance performance in moderately trained individuals. In contrast, data have been provided that elite athletes do not benefit from nitrate supplementation. The aim of this study was to evaluate the effects of short-term nitrate supplementation on endurance performance in subjects with different levels of aerobic fitness. METHODS Twenty-one subjects (mean age, 22.7 ± 1.8 yr) with different aerobic fitness level (V˙O2peak value ranging from 28.2 to 81.7 mL·kg·min) participated in a crossover double-blind placebo-controlled study. Subjects were tested after 6 d of supplementation with either 0.5 l per day of nitrate (5.5 mmol)-containing water (NITR) or nitrate-free water (PLA). Participants performed an incremental running test until exhaustion and four repetitions of 6-min submaximal (approximately 80% of gas exchange threshold) constant load exercise on a motorized treadmill. Moreover, subjects performed a 3-km running time trial on the field. RESULTS After NITR, a negative correlation between reduction of O2 cost of submaximal exercise and individual aerobic fitness level was observed (r = 0.80; P < 0.0001). A significant inverse correlation was also found between aerobic fitness level and improvement in performance for 3-km time trial after NITR (r = 0.76; P < 0.0001). Additionally, subjects responded differently to dietary nitrate supplementation according to aerobic fitness level with higher-fit subjects showing a lower increase in plasma [NO3] (r = 0.86; P < 0.0001) and [NO2] (r = 0.75; P < 0.0001). CONCLUSIONS The results of the present study suggest that the individual aerobic fitness level affects the ergogenic benefits induced by dietary nitrate supplementation. The optimal nitrate loading regimen required to elevate plasma [NO2] and to enhance performance in elite athletes is different from that of low-fit subjects and requires further studies.

Time-Course Changes of Oxidative Stress Response to High-Intensity Discontinuous Training versus Moderate-Intensity Continuous Training in Masters Runners

Beneficial systemic effects of regular physical exercise have been demonstrated to reduce risks of a number of age-related disorders. Antioxidant capacity adaptations are amongst these fundamental changes in response to exercise training. However, it has been claimed that acute physical exercise performed at high intensity (>60% of maximal oxygen uptake) may result in oxidative stress, due to reactive oxygen species being generated excessively by enhanced oxygen consumption. The aim of this study was to evaluate the effect of high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on oxidative damage. Twenty long-distance masters runners (age 47.8±7.8 yr) on the basis of the individual values of gas exchange threshold were assigned to a different 8-weeks training program: continuous moderate-intensity training (MOD, n = 10) or HIDT (n = 10). In both groups before (PRE) and after (POST) training we examined the following oxidative damage markers: thiobarbituric acid reactive substances (TBARS) as marker of lipid peroxidation; protein carbonyls (PC) as marker of protein oxidation; 8-hydroxy-2-deoxy-guanosine (8-OH-dG) as a biomarker of DNA base modifications; and total antioxidant capacity (TAC) as indicator of the overall antioxidant system. Training induced a significant (p<0.05) decrease in resting plasma TBARS concentration in both MOD (7.53±0.30 and 6.46±0.27 µM, PRE and POST respectively) and HIDT (7.21±0.32 and 5.85±0.46 µM, PRE and POST respectively). Resting urinary 8-OH-dG levels were significantly decreased in both MOD (5.50±0.66 and 4.16±0.40 ng mg−1creatinine, PRE and POST respectively) and HIDT (4.52±0.50 and 3.18±0.34 ng mg−1creatinine, PRE and POST respectively). Training both in MOD and HIDT did not significantly modify plasma levels of PC. Resting plasma TAC was reduced in MOD while no significant changes were observed in HIDT. In conclusion, these results suggest that in masters runners high-intensity discontinuous does not cause higher level of exercise-induced oxidative stress than continuous moderate-intensity training, inducing similar beneficial effects on redox homeostasis.

Training Effects on ROS Production Determined by Electron Paramagnetic Resonance in Master Swimmers

Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P < 0.01) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus 3.28 ± 0.66 µmol·min−1) was observed. HIDT increased peak oxygen consumption (36.1 ± 4.3 versus 40.6 ± 5.7 mL·kg−1·min−1 PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (−20%) and after IE (−25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.

Effects of a Short-Term High-Nitrate Diet on Exercise Performance

It has been reported that nitrate supplementation can improve exercise performance. Most of the studies have used either beetroot juice or sodium nitrate as a supplement; there is lack of data on the potential ergogenic benefits of an increased dietary nitrate intake from a diet based on fruits and vegetables. Our aim was to assess whether a high-nitrate diet increases nitric oxide bioavailability and to evaluate the effects of this nutritional intervention on exercise performance. Seven healthy male subjects participated in a randomized cross-over study. They were tested before and after 6 days of a high (HND) or control (CD) nitrate diet (~8.2 mmol∙day−1 or ~2.9 mmol∙day−1, respectively). Plasma nitrate and nitrite concentrations were significantly higher in HND (127 ± 64 µM and 350 ± 120 nM, respectively) compared to CD (23 ± 10 µM and 240 ± 100 nM, respectively). In HND (vs. CD) were observed: (a) a significant reduction of oxygen consumption during moderate-intensity constant work-rate cycling exercise (1.178 ± 0.141 vs. 1.269 ± 0.136 L·min−1); (b) a significantly higher total muscle work during fatiguing, intermittent sub-maximal isometric knee extension (357.3 ± 176.1 vs. 253.6 ± 149.0 Nm·s·kg−1); (c) an improved performance in Repeated Sprint Ability test. These findings suggest that a high-nitrate diet could be a feasible and effective strategy to improve exercise performance.

Effects of Manipulating Volume and Intensity Training in Masters Swimmers

PURPOSE Recently, some studies have suggested that overall training intensity may be more important than training volume for improving swimming performance. However, those studies focused on very young subjects, and/or the difference between high-volume and high-intensity training was blurred. The aim of this study was to investigate in masters swimmers the effects of manipulation of training volume and intensity on performance and physiological variables. METHODS A group of 10 male masters swimmers (age 32.3 ± 5.1 y) performed 2 different 6-wk training periods followed by 1 wk of tapering. The first period was characterized by high training volume performed at low intensity (HvLi), whereas the second period was characterized by low training volume performed at high intensity (LvHi). Peak oxygen consumption (VO2peak) during incremental arm exercise, individual anaerobic threshold (IAT), and 100-m, 400-m, and 2000-m-freestyle time were evaluated before and at the end of both training periods. RESULTS HvLi training significant increased VO2peak (11.9% ± 4.9% [mean change ± 90%CL], P = .002) and performance in the 400-m (-2.8% ± 1.8%, P = .002) and 2000-m (-3.4% ± 2.9%, P = .025), with a likely change in IAT (4.9% ± 4.7%, P > .05). After LvHi training, speed at IAT (12.4% ± 5.3%, P = .004) and 100-m performance (-1.2% ± 0.8%, P = .001) also improved, without any significant changes in VO2peak, 2000-m, and 400-m. CONCLUSIONS These findings indicate that in masters swimmers an increase of training volume may lead to an improvement of VO2peak and middle- to long-distance performance. However, a subsequent period of LvHi training maintains previous adjustments and positively affects anaerobic threshold and short-distance performance.

Temporal analisys of men’s single badminton matches played under different scoring systems

SISMES VII NATIONAL CONGRESS Padova 2–4 October 2015 THE MARCELLO FAINA LECTURE Citius, altius, fortius: beneficial effects of resistance training for children and adolescentsThe new scoring system (5x11) proposed by the Badminton World Federation, despite a similar physiological commitment with respect to the actual system (3x21), requires a lower total EE. Therefore, according to the present findings, this new scoring system should be adopted, some changes in training modalities need to be introduced. Ramp Test (mean ± SD) VO2 peak (ml·min -1) 3661 ± 328 VO2 peak (mlO2·min -1·kg-1) 54.8 ± 6.7 VEpeak (l·min -1) 152 ± 14SISMES VII NATIONAL CONGRESS Padova 2–4 October 2015 THE MARCELLO FAINA LECTURE Citius, altius, fortius: beneficial effects of resistance training for children and adolescents

Comparison of the physiological responses to matches differing in scoring system in badminton professional players

SISMES VII NATIONAL CONGRESS Padova 2–4 October 2015 THE MARCELLO FAINA LECTURE Citius, altius, fortius: beneficial effects of resistance training for children and adolescentsThe new scoring system (5x11) proposed by the Badminton World Federation, despite a similar physiological commitment with respect to the actual system (3x21), requires a lower total EE. Therefore, according to the present findings, this new scoring system should be adopted, some changes in training modalities need to be introduced. Ramp Test (mean ± SD) VO2 peak (ml·min -1) 3661 ± 328 VO2 peak (mlO2·min -1·kg-1) 54.8 ± 6.7 VEpeak (l·min -1) 152 ± 14SISMES VII NATIONAL CONGRESS Padova 2–4 October 2015 THE MARCELLO FAINA LECTURE Citius, altius, fortius: beneficial effects of resistance training for children and adolescents

Effects of nitrate supplementation on repeated sprint performance in healthy subjects

SISMES – V NATIONAL CONGRESS Pavia, 27 -29 September 2013 FUNCTIONAL ADAPTATION IN SPORT AND EXERCISE

Physical fitness and academic performance in high school students

SISMES – V NATIONAL CONGRESS Pavia, 27 -29 September 2013 FUNCTIONAL ADAPTATION IN SPORT AND EXERCISE

Cardiovascular and metabolic responses at rest and to exercise during 48 hours of head-out immersion: a case report

Head-out immersion in water (HIW) leads to several cardiovascular adjustments. Cardiac output increases by increasing stroke volume with no substantial changes in heart rate (HR) at rest. Therefore, total peripheral resistance to blood flow declines, leading to elevated O2 delivery. During exercise, cardiac output increases for a given workload while the HR response differs in relation to exercise intensity: it does not change during moderate exercise, but decreases during intense exercise. Oxygen uptake does not change either at rest or during exercise. In this case report, we report data on the cardiovascular and metabolic responses in a 29-year-old man at rest and during constant-load exercise during 48 h of HIW. The subject performed constant-load exercise on cycloergometer at 50% VO2max in the laboratory and during 48 h of HIW. Pulmonary gas exchange, VO2 kinetics, HR and rate of perceived exertion were determined. At rest, HR was similar but