Lal Singh

In vitro morphogenesis studies in gerbera jamesonii bolus ex hooker F.

An experiment was conducted during 2013 at the Horticultural Biotechnology Laboratory, KNK College of Horticulture, Mandsaur, RVSKVV, Gwalior (MP). The highest percentage of aseptic culture (83.54%) of explants were recorded with 0.5% bavistin in combination with 0.2% HgCl2 when diverse explants were exposed for 30 minutes. followed by application of 0.5% bavistin in combination with 0.1% HgCl2 (78.76%), while higher survival percentage (74.32%) of explants were evidenced with 0.5% bavistin in combination with 0.1% HgCl2 with exposure time 30 minutes. Combination of 0.5% bavistin with 0.1% HgCl2 exposed for the 30 minutes supported higher percentage of aseptic culture (78.76%) as well as survival of explants (74.13%) so that, this concentration and combination was used for surface sterilization of explants of gerbera during present investigation. The maximum callus was recorded on culture medium MS3D (35.09%), MS3N (33.83%) and MS2D (33.02%) with at par performance. Remaining culture media had low callus forming ability including MS5T (18.87%) that has significantly minimum counts. Maximum callus induction were recorded from cultured mature embryo on nutrients media MS3ip (35.06%) closely followed by a group of four nutrients media viz: MS2ip (29.95%), MSip (29.75%), MS.5ip (29.93%) and MS.2ip (29.12%). different culture media combination, inoculation media MS2N.5ip (55.22%), MS3N.5ip (53.17%) and MSN5ip (45.01%) were proved remarkably superior for shoots proliferating efficiency. Nutrients media MS5D.5Kn (3.05%) and MS4D.5KN (4.81%) was found low responding in this regard. Nutrient media MS2N (2.14cm), MSN (2.03cm) and MS2D (1.95) had produced shoot of higher length.