Lariane O. Cargnelutti
Universidade Federal de Santa Maria
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Lariane O. Cargnelutti.
Inflammation | 2013
Karine Santos De Bona; Gabriela Bonfanti; Paula Eliete Rodrigues Bitencourt; Lariane O. Cargnelutti; Priscila S. da Silva; Leidiane de Lucca; Victor Camera Pimentel; Etiane Tatsch; Thissiane L. Gonçalves; Melissa Orlandin Premaor; Rafael Noal Moresco; Maria Beatriz Moretto
Metabolic syndrome (MetS) leads to changes in enzymatic activities, oxidative and inflammatory parameters. Adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), butyrylcholinesterase (BuChE) and γ-glutamyltransferase (γ-GT) activities, C-reactive protein (hsCRP) and nitric oxide levels (NOx), as well as oxidative stress markers were analyzed in 39 subjects with MetS and 48 controls. Also, the influence of body mass index (BMI) and anthropometric measurements were evaluated. Disturbances in antioxidant defenses and higher γ-GT and BuChE activities, NOx and hsCRP levels were observed in subjects with MetS. These findings remained associated with MetS after adjustment for BMI, except for hsCRP. ADA was correlated with age, insulin levels and HOMA-IR index in MetS. DPP-IV and total cholesterol (TC), BuChE activity and TC, and VIT C and hsCRP levels also were correlated. The analyzed parameters may reflect the inflammatory state of the MetS, and could contribute to prevention and control of various aspects of this syndrome.
Journal of Complementary and Integrative Medicine | 2015
Paula Eliete Rodrigues Bitencourt; Karine Santos De Bona; Lariane O. Cargnelutti; Gabriela Bonfanti; Aline S. Pigatto; Aline Augusti Boligon; Margareth Linde Athayde; Felipe Pierezan; Régis Adriel Zanette; Maria Beatriz Moretto
Abstract Background: The effects of the aqueous seed extract of Syzygium cumini (ASc) in a short-term model of diabetes in rats are little explored. The present study was designed to evaluate the effect of the ASc on adenosine deaminase (ADA) activity and on biochemical and histopathological parameters in diabetic rats. Methods: ASc (100 mg/kg) was administered for 21 days in control and streptozotocin (STZ)-induced (60 mg/kg) diabetic rats. ADA activity, lipoperoxidation (cerebral cortex, kidney, liver and pancreas) and biochemical (serum) and histopathological (pancreas) parameters were evaluated. Results: The main findings in this short-term model of Diabetes mellitus (DM) were that the ASc (i) significantly reverted the increase of ADA activity in serum and kidney; (ii) ameliorated the lipoperoxidation in the cerebral cortex and pancreas of the diabetic group; (iii) demonstrated hypolipidemic and hypoglycemic properties and recovered the liver glycogen; and iv) prevented the HOMA-IR index increase in the diabetic group. Therefore, the ASc can be a positive factor for increasing the availability of substrates with significant protective actions, such as adenosine. Moreover, by maintaining glycogen and HOMA-IR levels, the extract could modulate the hyperglycemic state through the direct peripheral glucose uptake. Conclusions: Our data revealed that the short-term treatment with ASc has an important protective role under pathophysiological conditions caused by the early stage of DM. These results enhance our understanding of the effect of the ASc on the purinergic system in DM.
Human & Experimental Toxicology | 2013
Per Bitencourt; Luziane Potrich Bellé; Gabriela Bonfanti; Lariane O. Cargnelutti; K.S. De Bona; Priscila S. da Silva; Fátima H. Abdalla; Régis Adriel Zanette; Rb Guerra; C. Funchal; Maria Beatriz Moretto
Selenium (Se) has anti-inflammatory and antioxidant properties and is necessary for the development and normal function of the central nervous system. This study was aimed to compare the in vitro effects of 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one (C21H2HOSe; organoselenium) and sodium selenate (inorganic Se) on adenosine deaminase (ADA) activity, cell viability, lipid peroxidation, scavenger of nitric oxide (NO) and nonprotein thiols (NP-SH) content in the cerebral cortex slices of the young rats. A decrease in ADA activity was observed when the slices were exposed to organoselenium at the concentrations of 1, 10 and 30 µM. The same compound showed higher scavenger capacity of NO than the inorganic compound. Inorganic Se was able to protect against sodium nitroprusside-induced oxidative damage and increased the NP-SH content. Both the compounds displayed distinctive antioxidant capacities and were not cytotoxic for the cerebral cortex slices in the conditions tested. These findings are likely to be related to immunomodulatory and antioxidant properties of this compound.
Clinical Biochemistry | 2012
Karine Santos De Bona; Gabriela Bonfanti; Lariane O. Cargnelutti; Paula Eliete Rodrigues Bitencourt; Priscila S. da Silva; Ronise B. Ceolin; Victor Camera Pimentel; Maria Beatriz Moretto
OBJECTIVES Metabolic syndrome (MetS) is considered a state of chronic inflammation. This study aimed to ascertain selected parameters of purinergic and cholinergic systems related to glucose metabolism and inflammation, as well as (γ)-glutamyltransferase (GGT) and N-acetyl-b-glucosaminidase (NAG) activities and lipoperoxidation in lymphocytes of patients with MetS. DESIGN AND METHODS The adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), acetylcholinesterase (AChE), GGT and NAG activities, as well as thiobarbituric acid reactive substances (TBARS) levels were investigated in lymphocytes of patients with MetS (n=38) and healthy volunteers (n=41). We also evaluated the insulin levels, anthropometric measurements and routine biochemical analyses. RESULTS ADA (p<0.05), DPP-IV and AChE (p<0.0001) activities were higher in patients with MetS when compared to the control group. Furthermore, we observed correlations between ADA and DPP-IV activities (p=0.0002; r=0.5945), TBARS levels and ADA (p=0.0021; r=0.5172) and DPP-IV activities (p=0.0022; r=0.5010). CONCLUSIONS Our findings showed that MetS might cause tissue distress that disturbed lymphocytic ADA, DPP-IV and AChE activities in response to inflammatory stimuli. These alterations evidence clinical abnormalities, since these enzymatic systems are able to regulate several aspects of adipose tissue function and inflammatory state of MetS and could be used successfully both for preventing and for halting the progression of MetS.
Pharmaceutical Biology | 2017
Paula Eliete Rodrigues Bitencourt; Lariane O. Cargnelutti; Carolina S. Stein; Raquel Lautenchleger; Luana Mota Ferreira; Manuela B. Sangoi; Laura Bedin Denardi; Raphaela Maleski Borges; Aline Augusti Boligon; Rafael Noal Moresco; Letícia Cruz; Régis Adriel Zanette; Sydney Hartz Alves; Maria Beatriz Moretto
Abstract Context: Syzygium cumini (L.) Skeels (Myrtaceae) is a medicinal plant widely used in folk medicine for the treatment of diabetes mellitus (DM). However, studies on the use of this plant and of nanoparticle formulations against DM-related fungal infections are scarce. Objective: To evaluate the effect of the treatments with aqueous seed extract of S. cumini (ASc) and ASc-loaded polymeric nanoparticles (NPASc) on biochemical parameters in Candida albicans-infected diabetic rats. Materials and methods: Male Wistar rats were divided into eight groups: Control, DM, C. albicans, C. albicans + ASc, C. albicans + NPASc, DM + C. albicans, DM + C. albicans + ASc and DM + C. albicans + NPASc. Rats were daily treated with ASc or NPASc (100 mg/kg) for 21 days. Biochemical parameters in serum and urine, advanced oxidation protein product (AOPP) and TBARS levels in the serum, kidney, liver and pancreas and N-acetyl-β-d-glucosaminidase (NAG) activities in kidney and urine were evaluated. Results: Biochemical and oxidative stress parameters increased in rats with DM and/or candidiasis. NPASc was more effective than ASc in decreasing glucose (56%), cholesterol (33%) and creatinine (51%) levels; serum (16%) and pancreatic (46%) AOPP and renal (48%) TBARS levels when compared with DM + C. albicans group. In C. albicans group, both treatments decreased NAG activity but did not decrease creatinine levels. Conclusions: These data suggest that the use of nanotechnology is able to improve plant extract properties such as antioxidant activity that may be useful in diabetes-related complications.
journal of applied pharmaceutical science | 2017
Paula Er Bitencourt; Lariane O. Cargnelutti; Carolina S. Stein; Raquel Lautenchleger; Luana Mota Ferreira; Manuela B. Sangoi; Aline Augusti Boligon; Marta Mmf Duarte; Rafael Noal Moresco; Letícia Cruz; Régis Adriel Zanette; Sydney Hartz Alves; Maria Beatriz Moretto
1 Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. 2 Departamento de Farmácia Industrial, Centro de Ciências da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, Brazil. 3 Departamento de Microbiologia, Centro de Ciências da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, Brazil. 4 Universidade Luterana do Brasil, Santa Maria, Brazil. 5 Programa de Pós-Graduação em Ciências Biológicas: Farmacologia e Terapêutica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Brazilian Journal of Pharmaceutical Sciences | 2016
Gabriela Bonfanti; Paula Eliete Rodrigues Bitencourt; Karine Santos De Bona; Luis Ricardo Peroza; Lariane O. Cargnelutti; Raphaela Maleski Borges; Aline S. Pigatto; Roselei Fachinetto; Aline Augusti Boligon; Margareth Linde Athayde; Thissiane L. Gonçalves; Maria Beatriz Moretto
Solanum guaraniticum is a medicinal plant traditionally used to treat gastric and liver diseases. However, there is no documented evidence corroborating its safety. The present study evaluated the potential toxicity of S. guaraniticum leaf extract after acute administration in rats. Single doses of the extract (1.250, 2.500, and 5.000 mg/kg) were administered by gavage, and the rats were then monitored for 48 h and/or 14 days. Mortality, acute signs of toxicity, and general activity in the open field test were assessed as well as hematological and biochemical parameters, enzymatic activity (δ-aminolevulinate dehydratase and acetylcholinesterase), and oxidative stress parameters (lipid peroxidation level, non-protein thiol content, tissue catalase activity, and serum ferrous reducing power). Phytochemical analysis was also performed by HPLC. The results showed that extract administration produced no deaths (LD50 > 5,000 mg/kg), and no significant adverse effects regarding food consumption, body weight gain, gross pathology, or other parameters. However, the open field tests showed a decrease in spontaneous activity (crossing and rearing) mainly at 48 h after treatment. The results suggest that S. guaraniticum extract is not acutely toxic, but causes alterations in central nervous system activity.
Revista Brasileira de Plantas Medicinais | 2016
E.O. Chielle; Gabriela Bonfanti; K.S. De Bona; Lariane O. Cargnelutti; Paula Eliete Rodrigues Bitencourt; P.S. Da Silva; M.M.A. Campos; Maria Beatriz Moretto
denosine deaminase (ADA) is a critical control point in the regulation of adenosine levels. This study aimed to investigate the effects of a polyphenolic flavonoid, rutin, on the activity of ADA in serum, the cerebral cortex, liver, kidney, and biochemical parameters in diabetic rats. The animals were divided into four groups (n=6) for the following treatments: control; diabetic (streptozotocin 55 mg/kg); diabetic with rutin (100 mg/kg/day); diabetic with glibenclamide (10 mg/kg/day). After 30 days, ADA activity and biochemical parameters were analyzed. The ADA activity in the serum was significantly elevated in the diabetic group compared to the control group (p<0.01). The treatment with rutin prevented the increase in ADA activity in the STZ-induced rats when compared to control group. Our data showed that rutin reduced glucose, LDL levels, and hepatic enzymes in comparison with the control group. These results demonstrate that the increase of ADA activity observed in diabetic rats may be an important indicator of the immunopathogenesis of hyperglycemic disorders and suggest that rutin is important for regulating the enzymatic activities associated with immune, hyperglycemic, and inflammatory response in diabetes mellitus.
Journal of Physiology and Biochemistry | 2014
Karine Santos De Bona; Gabriela Bonfanti; Paula Eliete Rodrigues Bitencourt; Lariane O. Cargnelutti; Priscila S. da Silva; Thainan Paz da Silva; Régis Adriel Zanette; Aline S. Pigatto; Maria Beatriz Moretto
Industrial Crops and Products | 2016
Paula Eliete Rodrigues Bitencourt; Luana Mota Ferreira; Lariane O. Cargnelutti; Laura Bedin Denardi; Aline Augusti Boligon; Michelli Fleck; Ricardo Brandão; Margareth Linde Athayde; Letícia Cruz; Régis Adriel Zanette; Sydney Hartz Alves; Maria Beatriz Moretto