Lars H. Pedersen

Photonic crystal fiber based evanescent-wave sensor for detection of biomolecules in aqueous solutions

We demonstrate highly efficient evanescent-wave detection of fluorophore-labeled biomolecules in aqueous solutions positioned in the air holes of the microstructured part of a photonic crystal fiber. The air-suspended silica structures located between three neighboring air holes in the cladding crystal guide light with a large fraction of the optical field penetrating into the sample even at wavelengths in the visible range. An effective interaction length of several centimeters is obtained when a sample volume of less than 1 microL is used.

Photonic crystal fiber long-period gratings for biochemical sensing

We present experimental results showing that long-period gratings in photonic crystal fibers can be used as sensitive biochemical sensors. A layer of biomolecules was immobilized on the sides of the holes of the photonic crystal fiber and by observing the shift in the resonant wavelength of a long-period grating it was possible to measure the thickness of the layer. The long-period gratings were inscribed in a large-mode area silica photonic crystal fiber with a CO2 laser. The thicknesses of a monolayer of poly-L-lysine and double-stranded DNA was measured using the device. We find that the grating has a sensitivity of approximately 1.4nm/1nm in terms of the shift in resonance wavelength in nm per nm thickness of biomolecule layer.

Selective detection of antibodies in microstructured polymer optical fibers

We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either alpha-streptavidin or alpha-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.

Localized biosensing with Topas microstructured polymer optical fiber

We present what is believed to be the first microstructured polymer optical fiber (mPOF) fabricated from Topas cyclic olefin copolymer, which has attractive material and biochemical properties. This polymer allows for a novel type of fiber-optic biosensor, where localized sensor layers may be activated on the inner side of the air holes in a predetermined section of the mPOF. The concept is demonstrated using a fluorescence-based method for selective detection of fluorophore-labeled antibodies.

Selective serial multi-antibody biosensing with TOPAS microstructured polymer optical fibers.

We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.

Molecular immobilization and detection in a photonic crystal fiber

No abstract

Photonic crystal fiber based antibody detection

An original approach for detecting labeled antibodies based on strong penetration photonic crystal fibers is introduced. The target antibody is immobilized inside the air-holes of a photonic crystal fiber and the detection is realized by the means of evanescent-wave fluorescence spectroscopy and the use of a transversal illumination setup.

Evanescent wave sensing using a hollow-core photonic crystal fiber

In order to realize an efficient absorption measurement based evanescent-wave sensor, a long interaction length and a strong penetration of the optical field into the sample space is required. For an optical fiber based device, with a solid silica core immersed into a liquid sample, the strength of the evanescent field increases with decreasing core radius. When the core diameter is comparable to the wavelength of the light, a large fraction of the light propagates in the evanescent field. We demonstrate evanescent-wave sensing on aqueous solutions of fluorophore labeled biomolecules positioned in the air holes of a hollow-core photonic crystal fiber (PCF). The aqueous solutions can be positioned in close proximity to light guided in small cores without removing the coating and cladding, thus ensuring a very robust device. In order to make selective DNA detection, we coated the inside of the hollow-core PCF with a sensing layer, which by hybridization selectively immobilize specific molecules. A fluorescence measurement method, where a line-shaped laser beam expose the fiber from the side and excites the fluorophore molecules, was realized. The emitted fluorescence tunnels via the evanescent field into the fiber core(s) and is analyzed by a spectrometer at the fiber end.

A microstructured polymer optical fiber biosensor

We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of the complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fibers.

Photonic Crystal Fiber Gratings: Prospects for Label-Free Biosensors

We study long-period gratings in photonic crystal fibers in the application as biosensors. The analyte can be infiltrated into the holes of the fiber and measured using evanescent-wave sensing principle.