Lars Olav Brandsæter

Sensitivity of Cirsium arvense to simulated tillage and competition

Abstract Single and combined effects were studied of root length, burial depth, cutting and competition from a seasonal green manure crop on the growth and development of C. arvense from roots found in the upper soil layer. The experiment was performed under field conditions and partly repeated in a green house. The green manure consisted of a mixture of phacelia, common vetch, red clover and Italian ryegrass. Root fragments of 5 and 10 cm length were buried at 5 and 15 cm soil depth, simulating soil tillage. Use of green manure alone reduced growth of C. arvense more than any other single factor (81–99%), while root length and burial depth were more variable in their effect (0–96% reduction). Combined treatments involving both green manure, deep root burial (15 cm) and short root length (5 cm) reduced growth of C. arvense consistently by 95–100%. At the minimum regenerative capacity of C. arvense the number of leaves was 3–7 in plots with no green manure, at which time cutting had the greatest effect on regrowth the following year. The level for minimum regenerative capacity was not possible to decide for C. arvense growing in competition with green manure. Combined multiple treatments in spring, including use of green manure and mechanical operations in the upper soil layers, appear to be a potential means of reducing C. arvense infestations.

Effect of Rhizome Fragmentation, Clover Competition, Shoot-Cutting Frequency, and Cutting Height on Quackgrass (Elymus repens)

Quackgrass is a problematic agricultural weed in the temperate zones of the world and is difficult to control without herbicides or intensive tillage. However, it may be possible to control quackgrass with less environmental impact by combining multiple low-intensity control methods. A pot experiment was conducted in July to October 2012 and repeated in June to September 2013 to investigate the effect of rhizome fragmentation, competition from white clover, shoot-cutting frequency, and cutting height on quackgrass. Rhizome fragmentation was expected to result in more, but weaker, quackgrass shoots that would be more vulnerable to shoot cutting and competition. However, by 20 d past planting, rhizome fragmentation did not change the total number of quackgrass shoots per pot, because an increase in main shoots was offset by a decrease in tiller numbers. Rhizome fragmentation did not reduce quackgrass biomass acquisition during the experimental period. Although rhizome fragmentation did reduce total fructan content, it did not enhance the effect of clover competition, shoot-cutting frequency, or shoot-cutting height. Clover competition by itself reduced quackgrass shoot numbers by 72%, rhizome biomass by 81%, and belowground fructan concentration by 10 percentage points, compared with no competition. The more frequently quackgrass shoots were cut, the less biomass quackgrass acquired, and a high shoot-cutting frequency (each time quackgrass reached 2 leaves) resulted in a lower belowground fructan concentration than a low shoot-cutting frequency (at 8 leaves). However, in pots without competition, a higher shoot-cutting frequency resulted in more quackgrass shoots. A lower shootcutting height (25 mm) had more impact when shoot cutting was more frequent. In conclusion, rhizome fragmentation did not reduce the number of quackgrass shoots or rhizome biomass, but competition from white clover, a high shoot-cutting frequency, and a low shoot-cutting height strongly suppressed quackgrass biomass and fructan acquisition. Nomenclature: Quackgrass; Elymus repens (L.) Gould; white clover; Trifolium repens L.

Low genetic variation of invasive Fallopia spp. in their northernmost European distribution range

Abstract Knowledge about the reproduction strategies of invasive species is fundamental for effective control. The invasive Fallopia taxa (Japanese knotweed s.l.) reproduce mainly clonally in Europe, and preventing spread of vegetative fragments is the most important control measure. However, high levels of genetic variation within the hybrid F. × bohemica indicate that hybridization and seed dispersal could be important. In Norway in northern Europe, it is assumed that these taxa do not reproduce sexually due to low temperatures in the autumn when the plants are flowering. The main objective of this study was to examine the genetic variation of invasive Fallopia taxa in selected areas in Norway in order to evaluate whether the taxa may reproduce by seeds in their most northerly distribution range in Europe. Fallopia stands from different localities in Norway were analyzed with respect to prevalence of taxa, and genetic variation within and between taxa was studied using amplified fragment length polymorphism (AFLP). Taxonomic identification based on morphology corresponded with identification based on simple sequence repeats (SSR) and DNA ploidy levels (8× F. japonica, 6× F. × bohemica and 4× F. sachalinensis). No genetic variation within F. japonica was detected. All F. × bohemica samples belonged to a single AFLP genotype, but one sample had a different SSR genotype. Two SSR genotypes of F. sachalinensis were also detected. Extremely low genetic variation within the invasive Fallopia taxa indicates that these taxa do not reproduce sexually in the region, suggesting that control efforts can be focused on preventing clonal spread. Climate warming may increase sexual reproduction of invasive Fallopia taxa in northern regions. The hermaphrodite F. × bohemica is a potential pollen source for the male‐sterile parental species. Targeted eradication of the hybrid can therefore reduce the risk of increased sexual reproduction under future warmer climate.

Rhizome Fragmentation by Vertical Disks Reduces Elymus repens Growth and Benefits Italian Ryegrass-White Clover Crops

Tillage controls perennial weeds, such as Elymus repens, partly because it fragments their underground storage organs. However, tillage is difficult to combine with a growing crop, which limits its application. The aim of this study was to evaluate how soil vertical cutting with minimum soil disturbance and mowing affect the growth and competitive ability of E. repens in a grass–clover crop. A tractor-drawn prototype with vertical disks was used to fragment E. repens rhizomes with minimal soil and crop disturbance. In experiments performed in 2014 and 2015 at a field site close to Uppsala, Sweden, the rhizomes were fragmented before crop sowing (ERF), during crop growth (LRF), or both (ERF+LRF). Fragmentation was combined with repeated mowing (yes/no) and four companion crop treatments (none, Italian ryegrass, white clover, and grass/clover mixture). The results showed that in the grass–clover crop, rhizome fragmentation reduced E. repens rhizome biomass production and increased Italian ryegrass shoot biomass. ERF and LRF both reduced E. repens rhizome biomass by about 38% compared with the control, while ERF+LRF reduced it by 63%. Italian ryegrass shoot biomass was increased by 78% by ERF, 170% by LRF and 200% by ERF+LRF. Repeated mowing throughout the experiment reduced E. repens rhizome biomass by about 75%. Combining repeated mowing with rhizome fragmentation did not significantly increase the control effect compared to mowing alone. We concluded that rhizome fragmentation using vertical disks can be used both before sowing and during crop growth to enhance the controlling effect of grass–clover crops on E. repens.

Sprouting Dynamics of Bunias orientalis

Bunias orientalis is a highly vigorous weed and the powerful taproot makes control difficult. Taking action against unwanted plant species at the development stage where they have a minimum of energy stored in their below ground plant parts has been efficient. Both young and older plants of B. orientalis were studied in order to identify this stage. Root dry-weight as a measurement of stored energy and the sprouting ability from root sections were studied as well as the transport direction of carbohydrates within the plants by using the isotop 14C.No dry-weight minimum of the roots was found. However, from the isotope experiment with 14C, changes in the transport direction of carbohydrate reserves were detected. The smallest proportion of 14C was located in the roots at the stage where the source/sink dynamic of carbohydrate reserves shifted from the below-ground plant parts being the source to being the sink. This was considered a weak point in the seasonal life cycle of the species.Young plants did not at any development stage show a defined drop in their regenerative capacity from root sections. Older plants had a minimum of regenerative capacity from root sections at the stage where the plants had started to elongate and had an average height of 26 cm and visible inflorescence. This corresponds well with the development stage found in the isotope experiment in which an indication of a weak point was identified.