Laura Brullé

Effects of a Non Thermal Plasma Treatment Alone or in Combination with Gemcitabine in a MIA PaCa2-luc Orthotopic Pancreatic Carcinoma Model

Pancreatic tumors are the gastrointestinal cancer with the worst prognosis in humans and with a survival rate of 5% at 5 years. Nowadays, no chemotherapy has demonstrated efficacy in terms of survival for this cancer. Previous study focused on the development of a new therapy by non thermal plasma showed significant effects on tumor growth for colorectal carcinoma and glioblastoma. To allow targeted treatment, a fibered plasma (Plasma Gun) was developed and its evaluation was performed on an orthotopic mouse model of human pancreatic carcinoma using a MIA PaCa2-luc bioluminescent cell line. The aim of this study was to characterize this pancreatic carcinoma model and to determine the effects of Plasma Gun alone or in combination with gemcitabine. During a 36 days period, quantitative BLI could be used to follow the tumor progression and we demonstrated that plasma gun induced an inhibition of MIA PaCa2-luc cells proliferation in vitro and in vivo and that this effect could be improved by association with gemcitabine possibly thanks to its radiosensitizing properties.

Antitumoral effects of combined non thermal plasma and gemcitabine treatments on a MIA PaCa-luc orthotopic pancreatic carcinoma model

Summary form only given. Cancer of the exocrine pancreas, especially ductal adenocarcinoma, the most common pancreatic cancer1, is rarely curable and has an overall survival rate of less than 4%. Moreover, chemotherapy and radiotherapy of pancreatic cancer are only, up to now, palliative treatments. New therapeutic approaches are then necessary. Recent results were obtained on the treatment of glioblastoma and colon carcinoma2,3 using non thermal plasma (NTP). They led us to assess the antitumoral effect of NTP alone or in combination with Gemcitabine, a, reference chemotherapeutic agent with radiosensitizing properties, on pancreatic cancer.

Abstract 2839: Antitumor activity on colorectal and pancreatic tumors of a new strategy based on ROS generation by non-thermal plasma

Background. Local treatments of tumors are mainly based on surgical resection and/or treatment such as photodynamic therapy (PDT) or ionizing radiation (IR). Action mechanisms of IR and PDT are based on the generation of ROS in the vicinity of the cells. In this context, cold or Non Thermal Plasma (NTP), a new technology which can generate in situ ROS, is currently under investigations. NTP is a cold ( Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2839. doi:1538-7445.AM2012-2839