Laura Maleci Bini

Insight into the structure and chemistry of glandular trichomes of Labiatae, with emphasis on subfamily Lamioideae

Glandular trichomes of Labiatae are among the most investigated secretory structures. Most species studied belong to subfamily Nepetoidae, including plants with aromatic properties, while so far a few species of subfamily Lamioideae were examined. In this work, we studied the micromorphology, ultrastructure, type and release of secretion of the glandular trichomes present on leaves and flowers of several species belonging to subfamily Lamioideae, (Stachys alopecuros (L.) Bentham subsp. alopecuros, S. officinalis (L.) Trevisan subsp. officinalis, S. germanica L. subsp. germanica, S. germanica L. subsp. salviifolia (Ten.) Gams, S. sylvatica L., S. heraclea All., S. plumosa Griseb., S. annua L., Prasium majus L., Sideritis romana L.) and one to the sister group Scutellarioideae (Scutellaria galericulata L.). Besides the well-known peltate and small capitate trichomes, widely described in the literature, other types of glandular trichomes were encountered; stalked peltate hairs and large capitate hairs. In particular, a new type of capitate trichome, exclusive of calices and corollas, which presents a mode and release of secretion never described before, is reported.

Characterization of Secondary Metabolites, Biological Activity and Glandular Trichomes of Stachys tymphaea Hausskn. from the Monti Sibillini National Park (Central Apennines, Italy)

Stachys tymphaea (Lamiaceae) is a perennial herb growing in forest openings and dry meadows of central and southern Italy. It was investigated for the first time here, determining the content of secondary metabolites, the micromorphology of glandular trichomes, the histochemical localization of secretion, and the biological activity of the volatile oil, namely, the cytotoxic, antioxidant, and antimicrobial properties. The plant showed a peculiar molecular pattern, being rich of biophenolic compounds as flavonoids, phenylethanoid glycosides, and caffeoylquinic acid derivatives, but poor of iridoids, which are known as marker compounds of the genus Stachys. The essential oil was characterized by GC‐FID and GC/MS analyses, revealing a high percentage of sesquiterpene hydrocarbons (54.6%), with germacrene D (30.0%) and (E)‐β‐farnesene (12.4%) as the most abundant compounds, while other main components were representatives of the diterpenes (19.2%), represented mainly by (E)‐phytol (11.9%). This composition supported the taxonomic relationships in the genus Stachys, which comprises oil‐poor species producing essential oils rich in hydrocarbons, with germacrene D as one of the predominant components. The micromorphological study revealed three types of glandular hairs, i.e., Type A peltate trichomes, being the primary sites of essential oil biosynthesis, Type B short‐stalked trichomes, typical mucopolysaccharide producers, and Type C long capitate trichomes, secreting a complex mixture of both lipophilic and hydrophilic substances, with a major phenolic fraction. Moreover, the MTT assay revealed the potential of the volatile oil to inhibit A375, HCT116, and MDA‐MB 231 tumor cells lines (IC50 values of 23.9–34.4 μg/ml).

Congruence of Phytochemical and Morphological Profiles along an Altitudinal Gradient in Origanum vulgare ssp. vulgare from Venetian Region (NE Italy)

Plants of Origanum vulgare L. ssp. vulgare from the Veneto region (NE Italy) were selected to study the variability of the essential‐oil composition from leaves and inflorescences throughout an elevation gradient. We investigated also the morphology of non‐glandular and glandular trichomes, their distribution on the vegetative and reproductive organs, as well as the histochemistry of the secreted products, with special focus on the terpenoidic fraction.

Business model disclosure in the Strategic Report: Entangling intellectual capital in value creation process

Purpose – The purpose of this paper is to evaluate business model (BM) disclosure. As the BM shows how a company creates and captures value, its communication in the Annual Report is considered a necessary background for a dynamic analysis, interpretation, and evaluation of the intellectual capital (IC) contribution to a company’s competitive advantage. Design/methodology/approach – Focusing on a sample of listed UK companies operating in high-technology industries, this paper runs a content analysis of BM disclosure presented in the Strategic Report (SR). To develop the analysis, it refers to an ontological approach that encompasses the main research contributions to this topic. Findings – The analysis of SRs revealed that few companies use their BM disclosure to highlight the contribution of their IC to create and capture value. BM descriptions are not always clearly distinguishable from other strategic concepts and poorly illustrate the interactions among the BM components, which help understand how IC...

Phytochemical Analysis, Biological Activity, and Secretory Structures of Stachys annua (L.) L. subsp. annua (Lamiaceae) from Central Italy

Stachys annua subsp. annua, well‐known in central Italy as ‘stregona annuale’, is an annual, small, slightly‐scented herb, commonly found in fields and uncultivated areas in almost all regions of Italy. In folk medicine, its aerial parts were used as anti‐catarrhal, febrifuge, tonic, and vulnerary. In the present work, the chemical composition of the flowering aerial parts was studied. The hydrodistilled volatile oil, analysed by GC/MS, showed sesquiterpenoids as the major fraction (42.5%); phytol (9.8%), germacrene D (9.2%), and spathulenol (8.5%) were the most abundant constituents. The volatile oil was assayed for antioxidant and cytotoxic activity by DPPH, ABTS, FRAP, and MTT methods. The cytotoxicity results against HCT116, A375, and MDA‐MB 231 human tumor cell lines were significant, with IC50 values of 23.5, 37.2, and 41.5 μg/ml, respectively, whereas the antioxidant power was negligible. The EtOH extract was composed mainly of three glycosidic flavonoids, namely 7‐{[2‐O‐(6‐O‐acetyl‐β‐D‐allopyranosyl)‐β‐D‐glucopyranosyl]oxy}‐5,8‐dihydroxy‐2‐(4‐methoxyphenyl)‐4H‐1‐benzopyran‐4‐one (1), 7‐{[6‐O‐acetyl‐2‐O‐(6‐O‐acetyl‐β‐D‐allopyranosyl)‐β‐D‐glucopyranosyl]oxy}‐2‐(3,4‐dihydroxyphenyl)‐5,8‐dihydroxy‐4H‐1‐benzopyran‐4‐one (2), and 7‐{[6‐O‐acetyl‐2‐O‐(β‐D‐allopyranosyl)‐β‐D‐glucopyranosyl]oxy}‐2‐(3‐hydroxy‐4‐methoxyphenyl)‐5,8‐dihydroxy‐4H‐1‐benzopyran‐4‐one (3). On the contrary, iridoids, considered chemotaxonomic markers of the genus Stachys, were absent in this species. Finally, the morphological and histochemical survey showed that glandular trichomes were composed of two main types, i.e. peltate type A and capitate types B and C giving positive response for both lipids and polyphenols.

Glandular Trichomes and Essential Oil Composition of Endemic Sideritis italica (Mill.) Greuter et Burdet from Central Italy

Sideritis italica (Mill.) Greuter et Burdet belongs to the Lamiaceae family and is endemic to Italy. The glandular trichomes (morphology, distribution, histochemistry, and ultrastructure) of the plant were studied for the first time, along with the chemical composition of the essential oils. Abundant non‐glandular hairs and peltate (type A) and capitate (types B, C1, and Cx) glandular trichomes were observed both on the vegetative and reproductive organs. The histochemical procedures and the ultrastructural investigation enabled specific location of the main site of essential oil production mainly in type‐A peltate hairs. Particular emphasis is given to the release mechanism of the secreted material in all of the types of glands, and the potential taxonomic value of the indumentum in the Lamiaceae family is briefly discussed. Essential oils were hydrodistilled from flowering aerial parts of S. italica, and 136 compounds (112 in flowerheads, 79 in vegetative parts) were identified. The quantitative prevalence of diterpenoids (43.4% in flowerheads and 22.3% in vegetative parts) was the most significant characteristic of the essential oil of S. italica that could be classified as a diterpene‐rich essential oil according to the classification of Kirimer.

Signalling Theory and Voluntary Disclosure to the Financial Market - Evidence from the Profitability Indicators Published in the Annual Report

Signalling theory posits that the most profitable companies provide the market with more and better information. The research, however, reveals disaccording results. Because the general disclosure level depends on many factors, our paper centres on a focal point of the signal that companies send to the financial market: the profitability indicators. As several studies have shown the strong relevance of this type of data, the hypothesis is that the most profitable companies should disclose more profitability indicators. A sample of UK and Italian firms has been selected to verify if signalling policies are adopted in two very different cultural, economic, and legal contexts. After controlling for size, risk, industry, and country, our results support our hypothesis. We conclude that the market is capable of controlling the production and use of information, concentrating it on the focal points of the agency relationship. Moreover, our results seem to dismiss the relevance of the European Directive 51/2003, which generally requires the company to communicate performance indicators. In fact, the most profitable companies communicate such data independent of any legal requirement. Less profitable companies, on the other hand, might be induced to massage their disclosure, presenting useless or doctored ratios.

Secretory structures and essential oil composition in Stachys officinalis (L.) Trevisan subsp. officinalis (Lamiaceae) from Italy

Abstract The secretory structures and the volatile fraction of Stachys officinalis (L.) Trevisan subsp. officinalis (Lamiaceae) from Italy were studied for the first time. Peltate and small capitate trichomes were observed on the whole plant (leaves and inflorescences). In the peltate trichomes, an unusual polyphenols content was evidenced by the histochemical methods. The volatile fraction was obtained by a solvent extract from the distillation water of leaves and inflorescences and analysed by GC-MS. Forty-four constituents for leaves, representing 94.1% of the total volatiles, and 57 compounds for flowers, accounting for 90.1% of the total volatiles, were identified. (E)-caryophyllene (20.1%), (E)-nerolidol (14.3%), caryophyllene oxide (6.1%) and γ-cadinene (5.7%) were recognised as the main constituents for the leaf volatile fraction, while caryophyllene oxide (16.5%), (E)-nerolidol (15.4%), humulene epoxide II (9.2%) and α-pinene (7.0%) were the main compounds for the flower volatile fraction.

Country Effects on European Mandatory Disclosure of Financial Key Performance Indicators.

European Union Directive 51/2003 requires the publication of Financial Key Performance Indicators (FKPIs) in order to standardize this practice. We aim to test whether and to what extent the directive realizes the standardization of FKPIs. Because country factors are obstacles that the international standardization process intends to remove, we study their influence on disclosure practices in two very different countries in terms of cultural, economic, and regulation environments: Italy and the United Kingdom. Disclosure practices involve two dimensions: the quantity of indicators published and their quality. Quality is measured in terms of compliance with qualitative characteristics advocated by the IASB’s Framework. We select a representative sample of listed companies in both countries. The FKPI quantity and quality are hand collected from their 2008 annual reports. After controlling for leverage, industry, size, and profitability, we find that the number of indicators published in an annual report does not vary by country. The Directive may have helped this process. On the other hand, country factors drive the quality of FKPI communication. Thus, this finding suggests that standard setters and regulators should shift their attention to the quality aspects of FKPIs.

Localization of phenolic compounds in the fruits of Silybum marianum characterized by different silymarin chemotype and altered colour

Silybum marianum (L.) Gaertn (Asteraceae) is a valuable medicinal plant utilized for silymarin production. However, only fragmentary and contradictory information about silymarin localization within S. marianum fruit are available. In this work, a twofold research approach was adopted in order to investigate the distribution and quantification of silymarin and of other phenolic compounds within the different fruit regions (pericarp, seed integument, cotyledon). Two S. marianum wild accessions with contrasting silymarin chemotype (A and B) and a mutant line (C) with an altered fruit colour were analysed. Fruits of Cynara cardunculus were studied as a reference. Firstly, the fruit morpho-anatomy was reviewed by means of light microscopy digital imaging and, secondly, a comprehensive histolocalization of the different classes of polyphenols within the fruit was carried out. The experimental evidences confirmed that silymarin, and its precursor taxifolin, are only accumulated in the seed integuments. The dark colour of fully-ripened fruits is due to the accumulation of condensed tannins in the pericarp subepidermal cell layer. On the contrary, the studied mutant line shows reduced condensed tannin content that probably result from impairment at the level of flavonoid biosynthetic pathway. Condensed tannins content is comparatively low in S. marianum fruits and very low in the identified mutant line. This could represent an advantage for the possible employment of S. marianum fruits and of silymarin extraction by-products in the feed and food sector.