Lawrence M. Marshall

Countercurrent distribution of labeled anomeric methyl arabinosides and methyl α-d-mannoside☆

Abstract The isotope effect observed during the countercurrent distribution of arabinose-1- 14 C did not appear with the methyl glycoside of this labeled pentose. This observation along with the difference in the symmetry of plots for methyl α- d -mannoside and d -mannose were considered in the discussion of such isotope effects.

Incorporation of 14C labeled glycine into yeast protein.

Abstract Torula utilis incorporates labeled carbon into its protein fractions by an enzymatic process. The ratio of this uptake is influenced by time, temperature, p H and disinfectants. This enzymatic uptake might be an expression of protein synthesis operating as an exchange reaction.

Dialysis of Certain Sugars through Cellophane

Of several sugars examined, alkali most affects the rate of dialysis through cellophane of alpha-beta-D-mannose and alpha-beta-maltose. The rates of dialysis of these two sugars are influenced by 0.01 and 0.1N solutions of sodium and potassium hydroxide. The rate of dialysis of sucrose is not influenced by the solutions employed.

In vitro carbon dioxide fixation into succinate

Abstract When succinate or 3-phosphoglyceric acid was incubated anaerobically with liver homogenates and 14 C labelled bicarbonate, the percentage of the total radioactivity in the chromatographically separated organic acids was greater in succinate than in any other acid. For the incubation with 3-phosphoglyceric acid, the specific activity of succinate, was greater than that of fumarate or malate. Aerobically the behaviour of 3-phosphoglyceric acid, with respect to carbon dioxide fixation into succinate, differed from that of pyruvate.

A Colorimetric Procedure for the Determination of Aspartic Acid.

Summary A procedure is described for the determination of aspartic acid in quantities of 0.5 mg or above in the sample. The amino acid is converted to fumaric acid and this is measured colorimetrically.