Leandro Nagae Kuritza

Óleo essencial de orégano, alecrim, canela e extrato de pimenta no controle de Salmonella, Eimeria e Clostridium em frangos de corte

The efficiency of a product in broiler feed containing essential oil of oregano, rosemary, cinnamon and extract of red pepper (plant compost) in the control of Salmonella, Eimeria and Clostridium was evaluated. Two experiments were carried out to evaluate the product. In the first experiment the efficiency of this product to control Clostridium perfringens after challenge with Eimeria acervulina, E. maxima and E. tenella was assessed. Day old chicks were allotted into three groups: T1 - control diet without growth promoter, T2 - diet with avilamycin (10ppm), and T3 - diet with addition of the plant compost (100ppm). The use of the plant compost in broiler diets reduced specific lesions of E. maxima and E. tenella at 14 days after inoculation and reduced the count of colony forming units (CFU) of Clostridium perfringens in the ceca comparing to the control group. In the second trial the efficiency of the same product in birds challenged with Salmonella Enteritidis was evaluated. Day old birds were submitted to three experimental diets: T1 - control diet without antibiotics growth promoter, T2 - diet with 10ppm Avilamycin, T3 - diet with 100ppm of the plant compost mentioned above. At 21 days of age all birds were inoculated with 105 CFU of Salmonella Enteritidis. The use of the plant compost and avilamycin decreased the excretion of Salmonella in poultry 72 hours after the inoculation. The use of the plant compost increased villous/CD3+ cells in the duodenum, compared to group avilamycin and control, but had no effect on the expression of these cells in the cecum.

Uso de probiótico sobre a ativação de células T e controle de Salmonella Minnesota em frangos de corte

Para avaliar o efeito do probiotico sobre a resposta imunologica de frangos de corte desafiados com Salmonella Minnesota (SM), 60 frangos foram divididos em tres grupos: CN- (controle negativo) aves que nao foram inoculadas com SM, CP- (controle positivo) aves inoculadas com SM e Probiotico- aves suplementadas na racao com probiotico composto de Lactobacillus acidophilus, L. plantarium, L. rhamnosus, L. bulgaricus, Enterococcus faecium, Streptococcus thermophilus e Bifidobacterium bifidum e desafiadas com SM. Aos 14 dias foi realizada a inoculacao com SM e aos 7 e 35 dias foram quantificadas celulas caliciformes, CD4+ e CD8+ na mucosa intestinal do ileo e ceco. Aves suplementadas com probioticos aos 7 dias de idade apresentaram aumento significativo (P≤0,05) de celulas caliciformes e CD4+ no ileo e de celulas CD8+ no ceco. Aos 35 dias houve aumento significativo (P≤0,05) das celulas CD8+ nas aves inoculadas do CN e Probiotico. A utilizacao de probioticos proporcionou reducao significativa (P≤0,05) da contagem de Salmonella sp.