Leif K. Bakland

The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer

Various organic acids, ultrasonic instruments, and lasers have been used to remove the smear layer from the surface of instrumented root canals. The purpose of this study was to investigate the effect of various concentrations of sodium hypochlorite (NaOCl) as an intracanal irrigant before the use of MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) as a final rise to remove the smear layer. Ten operators, using a combination of passive step-back and rotary 0.04 taper, nickel-titanium files, prepared 80 single- and multirooted human teeth. Distilled water, four different concentrations of NaOCl, or MTAD was used as intracanal irrigant. The canals were then treated for 2 min with 5 ml of one of the following solutions as a final rinse: 5.25% NaOCl, sterile distilled water, 17% EDTA, or MTAD. The presence or absence of smear layer and the amount of erosion on the surface of the root canal walls at the coronal, middle, and apical portion of each canal were examined under a scanning electron microscope. The results show that although MTAD removes most of the smear layer when used as an intracanal irrigant, some remnants of the organic component of the smear layer remain scattered on the surface of the root canal walls. The effectiveness of MTAD to completely remove the smear layer is enhanced when low concentrations of NaOCl are used as an intracanal irrigant before the use of MTAD as a final rinse. This regimen does not seem to significantly change the structure of the dentinal tubules.

Measurement of Adhesion of Endodontic Sealers to Dentin

The bond strength of root canal sealers to dentin seems to be an important property for maintaining the integrity of the seal of root-canal fillings. In the few studies published, various assessment methods were used so quantitative comparison is not possible. The purpose of this study was to develop an effective and easily reproducible model and to test it with nine contemporary, commercially available endodontic sealers. After slicing off the coronal 2 mm of extracted third molars, the exposed dentin served as test surfaces. The teeth were fixed with plaster in 1-inch phenolic rings. Five-mm long sections of polyethylene tubing, filled with freshly mixed sealer, were placed on the dentin and tested for shearing bond strength after setting. A custom-made holder allowed the rings to be attached to an Instron machine, activated at a cross-arm speed of 0.5 mm/min. The mean bond strength, ranged from 0 to 4.9 MPa. The sealers were ranked and those that did not differ statistically in their bond strength were grouped together. This model provides a simple and reproducible means for measuring the in vitro bond strength of endodontic sealers.

Anatomical relationship of the mandibular canal to its surrounding structures in mature mandibles.

A lack of agreement exists in the literature regarding the anatomical relationship of the mandibular canal to its surrounding structures such as the root apices. The purpose of this investigation was to study the spatial relationship of the mandibular canal to the posterior teeth in dried human mandibles. Twenty-two mature dried mandibles were sectioned through the root apices of the first and second premolars and molars. Second premolars and second molars had the closest distances to the canal with a mean of 4.7 mm and 3.7 mm, respectively. With a mean of 6.9 mm, the apices of the mesial roots of the first molars were farthest from the canal. The canal pathway in mature mandibles followed in S-shaped curve in 31% of the cases. In 41% of the cases it was located lingual (19%), buccal (17%), or directly inferior (5%) to the apices of the posterior teeth. In 28% of the cases the canal could not be identified clearly in the second premolar and first molar regions. In a typical S-shaped configuration the canal was located buccal to the distal root of the second molar, crossed to the lingual below the second molar mesial root, ran lingual to the first molar, and crossed back to the buccal apical to the apex of the second premolar. Based on our results it appears that the mandibular second premolar and second molar are the most likely teeth to be involved in accidental damage to the mandibular canal during root canal therapy.

Will mineral trioxide aggregate replace calcium hydroxide in treating pulpal and periodontal healing complications subsequent to dental trauma? A review

Mineral trioxide aggregate (MTA) has over the last two decades begun to take the place of calcium hydroxide (CH) in the treatment of a variety of pulpal and periodontal healing complications following dental trauma. These conditions include teeth with: (i) exposed pulps, (ii) immature roots and pulp necrosis, (iii) root fractures and pulp necrosis located in the coronal part of the pulps, and (iv) external infection-related (inflammatory) root resorption. The main reasons for replacing CH with MTA in these situations have generally been the delayed effect when using CH to induce hard tissues, the quality of such induced hard tissues, and finally the dentin weakening effect of CH, which in some instances lead to cervical root fractures in immature teeth. MTA appears, from a relatively few clinical studies, to overcome these shortcomings of CH. The lack of long-term clinical studies, however, may warrant a certain reservation in an unrestricted replacement of CH with MTA. A definite need for randomized clinical studies comparing CH and MTA in trauma healing situations is urgently needed.

Immunopathogenesis of chronic periapical lesions: A review

The pathogenesis of periapical lesions has not been fully elucidated. Currently, the possibility of its being an immunologic phenomenon is receiving much attention. This article presents a review of the literature concerning immunologic reactions which may involve periapical lesions. It appears that antigen-antibody complexes and IgE-mediated reactions can initiate preliminary changes in periapical tissues. It is also likely that delayed hypersensitivity participates in the perpetuation and progression of periapical disease.

The concentration of prostaglandin E2 in human periradicular lesions

Prostaglandins (PG) have been implicated in the genesis of periapical lesions. Periapical specimens from patients with clinical signs and symptoms of chronic and acute apical periodontitis were obtained and immediately frozen in liquid nitrogen. Periradicular tissues from unerupted third molars were frozen and used as controls. The concentration of PGE2 was determined by radioimmunoassay. Low levels of PGE2 were found in the control tissues as compared with those detected in chronic and acute lesions. Significantly higher levels of PGE2 were found in acute lesions than those found in chronic lesions. The results show that acute lesions have higher concentrations of PGE2 than chronic lesions and confirm the role of prostaglandins in the pathogenesis of human periapical lesions.

Pulp regeneration after non-infected and infected necrosis, what type of tissue do we want? A review

Regeneration (revitalization) of infected necrotic pulp tissue has been an important issue in endodontics for more than a decade. Based on a series of case reports, there appears to be evidence that new soft tissue can enter the root canal with a potential for subsequent hard tissue deposition resulting in a narrowing of the root canal. Very little is presently known about the exact nature of this tissue growing into the canal and how it may behave in the long term. In the case of regeneration of necrotic non-infected pulp tissue, a series of clinical and histological studies have shown that such events may take place in four variants: (i) Revascularization of the pulp with accelerated dentin formation leading to pulp canal obliteration. This event has a good long-term prognosis. (ii) Ingrowth of cementum and periodontal ligament (PDL). The long-term prognosis for this event is not known. (iii) Ingrowth of cementum, PDL, and bone. The long-term prognosis is only partly known, but cases developing an internal ankylosis have been described. (iv) Ingrowth of bone and bone marrow is a rare phenomenon and the long-term prognosis does not appear to be good. Based on current knowledge, expectations with respect to pulp regeneration (revitalization) of infected necrotic dental pulps are difficult to predict; more information than now available is needed before procedures for pulpal regeneration can be routinely recommended with a predictable long-term prognosis.

Prostaglandins: their possible role in the pathogenesis of pulpal and periapical diseases, part 2

This paper presents a review of prostaglandins, including history, chemical structure, biosynthesis, physiologic and pathologic actions, and inhibitors and antagonists. These biologically active substances appear to be involved in the pathogenesis of pulpal and periapical diseases. However, the exact nature of their involvement remains to be investigated.

Nonendodontic therapy for supraosseous extracanal invasive resorption

Supraosseous extracanal invasive resorption involves cervical root dentin in a resorptive process that does not invade the pulp. A treatment procedure is described wich permits pulp preservation; the technique is illustrated with case histories.

Localization of IgE immunoglobulin in human dental periapical lesions by the peroxidase-antiperoxidase method

Abstract Twenty-eight dental periapical lesions were examined for the presence of light chains of human immunoglobulins, IgG, and IgE by the peroxidase-antiperoxidase method; 27 stained positively for light chains and IgG and 20 for the presence of IgE. One tissue specimen, diagnosed histologically as periapical scar tissue, contained no immunoglobulins. The presence of IgE immunoglobulin in 74 per cent of periapical tissue specimens suggests that IgE-mediated reactions participate in pathogenesis of human dental periapical lesions.