Leon Goldstein

Use of water-insoluble enzyme derivatives in biochemical analysis and separation

SummaryA brief review of the most commonly employed preparative procedures for water-insoluble enzyme derivatives is given, followed by a description of the various factors which can affect the behaviour and the apparent kinetic parameters of an immobilized enzyme system. Current developments in the application of water-insoluble enzyme derivatives in biochemical analysis and separation are dealt with.ZusammenfassungEin Überblick über die gebräuchlichsten Methoden zur Herstellung wasserunslöslicher Enzymderivate wird gegeben. Anschließend werden die verschiedenen Faktoren beschrieben, die das Verhalten und die scheinbaren kinetischen Parameter eines immobilisierten Enzymsystems beeinflussen. Die Anwendungsmöglichkeiten wasserunlöslicher Enzymderivate in der biochemischen Analytik werden besprochen.

Water-insoluble derivatives of naringinase

Abstract 1. 1. Water-insoluble derivatives of naringinasc were prepared by covalent linking of the enzyme to copolymers of maleic anhydride with ethylene, methylvinyl ether, isobutyl-vinyl ether, or styrene. 2. 2. The enzymic activity, mechanical properties, and stability of the various water-insoluble naringinase derivatives were investigated. 3. 3. Some effects of the carrier polymer side chains on the properties of the insoluble enzyme-polymer conjugates were clarified.