Leon V. McVay

A Study of Moniliasis in Aureomycin Therapy

Summary (1) In vitro sensitivity studies established a definite inhibitory effect by methyl and propyl paraben (para-hydroxy-benzoic acid) on 5 strains of yeast. (2) Methyl and propyl paraben are essentially non-toxic when administered orally, vaginally or rectally. (3) Methyl and propyl paraben are of value in preventing the overgrowth of C. albicans associated with aureomycin therapy.

A LONG-TERM EVALUATION OF AUREOMYCIN IN THE TREATMENT OF ACTINOMYCOSIS

Excerpt With the conquest of one infectious disease entity after another, attention has tended to concentrate on those conditions which have remained resistant to modern methods of therapy. Paramou...

ANTIBIOTIC PREVENTION OF INTERCURRENT INFECTIONS IN DIABETES MELLITUS

Excerpt With the steadily increasing number of elderly individuals in the general population, a greater interest in the diseases characteristic of later life has developed.1Outstanding among these ...

The treatment of Trichomonas vaginalis vaginitis with aureomycin.

Since we(1) had been successful in treating amebiasis with aureomycin it was thought possible that this antibiotic might be useful in other parasitic infestations. It was recalled that many amebicidal preparations are effective against T. vaginalis. Subsequent in vitro studies revealed that aureomycin is likewise trichomonicidal. Therefore, it was decided to investigate the action of the local application of aureomycin into the lower female genital tract in T. vaginalis vaginitis. A powder for vaginal insufflation was prepared by adding aureomycin hydrochloride to powdered talc (U.S.P. Merck). The first 6 patients were treated with talc alone. In each of these cases the vagina was insufflated with 2 g of powdered talc on 2 consecutive days and again on the fourth day. At the end of this period the vaginal secretions were negative for T. vaginalis in all 6 cases. Three days following cessation of therapy, the vagina contained T. vaginalis in 5 of the 6 cases. In the sixth patient vaginal secretions did not become positive until the eighth day. The leukorrhea and vaginal inflammation had persisted. Aureomycin in dosages of 2 grams a day for 7 days by mouth has been ineffective in the treatment of this condition. Through the cooperation of the Medical, Gynecological, and Obstetrical Services of the John Gaston Hospital 54 case of T. vaginalis vaginitis were obtained for treatment. Twelve of these patients were pregnant and were referred for treatment by the Obstetrical Service. The diagnosis in each case was established by the demonstration of the organism microscopically in the wet-mount preparation.

Isolation of Brucella from Apparently Healthy Individuals

In recent years the isolation of brucella organisms from cases of Hodgkins disease 1 has caused considerable discussion. The confirmation of this finding in our laboratory and the statements in the literature that these organisms could be isolated from patients with various infections 2 led us to seek an explanation. It seemed to us that the most likely explanation was that infection with brucella was widespread, at least in certain regions, and that the organism persisted in people furnishing favorable foci. In order to test this hypothesis it was decided to explore the possibility that tissues affording good conditions for growth of brucella organisms might actually harbor them. Since brucella are known to multiply in macrophages and fibroblasts, 3 it was thought that cultures of enlarged prostates and fibrosed fallopian tubes might reveal the presence of these organisms. At present 34 prostates have been cultured. From these cultures Brucella abortus has been isolated in 2 instances and Brucella melitensis in a third. Forty-three fallopian tubes have been similarly studied. From one of these Brucella melitensis has been isolated. The cultures of the prostates and tubes were made by obtaining from the surgeon, in a sterile container, portions of the prostate which were removed through the urethra. The specimens were immediately macerated, extracted, and the tissue extract incubated in Bacto-tryptose Broth at 37.5°C in 10% C02 for 10 days. After 10 days the broth specimens were inoculated on Bacto-Tryptose Agar plates, and these were similarly incubated for 10 days. Unless positive specimens were obtained sooner, this procedure was repeated twice before discarding the specimens as negative. Identification of the organisms was made by 1) the morphology of the colony on tryptose agar, 2) the morphology and staining reactions of the individual organisms, 3) sugar reactions, and 4) agglutination with specific antiserum.