Leonard P. Merkow

Pathogenesis of oncogenic simian adenoviruses. VII. The origin of annulate lamellae in LLC-MK2 cells infected with SV30.

LLC-MK2 cells were grown as monolayers, infected with the oncogenic simian adenovirus 30 (SV30), and harvested 1–192 hours post infection (p.i.). As early as 1 hour p.i., increased numbers of annulate lamellae were observed within the cytoplasm and nucleus of infected LLC-MK2 cells. The cytoplasmic annulate lamellae were usually in close proximity to the nucleus and frequently contiguous with the outer nuclear envelope. Intertubular connections between parallel lamellae, electron dense particles consistent with ribosomes, and crystalloid bodies in contact with annulate lamellae were observed. The increased number of annulate lamellae over that observed in control cultures may be either a cellular response concomitant with virus replication or a secondary manifestation of viral toxicity. The formation of annulate lamellae appears to occur by way of at least two processes in SV30-associated cells.

Temperature-Dependent Histotypic Changes in Walker Carcinosarcoma in the Chorioallantois

Solid tumors grew in the chorioallantois of chick embryos after the topical inoculation of ascites tumor cells. The microscopic character of the growth varied depending on the temperature of incubation. At 32.5�C a sarcomatous appearance was prominent; at 37.5�C carcinomatous structure in several alveolar patterns predominated; at 42.5�C growth was that of a giant cell carcinoma.