Lesley Galbraith

Lipopolysaccharides from Pseudomonas maltophilia: Composition of the lipopolysaccharide and structure of the side-chain polysaccharide from strain N.C.I.B. 9204

Lipopolysaccharide was extracted from defatted cell-walls of Pseudomonas maltophilia N.C.I.B. 9204. The major fatty acid components were 9-methyldecanoic acid, 2-hydroxy-9-methyldecanoic acid, 3-hydroxy-9-methyldecanoic acid, 3-hydroxy-dodecanoic acid, and 3-hydroxy-11-methyldodecanoic acid. Monosaccharide components of the phosphorylated core-oligosaccharide were D-glucose, D-mannose, D-galacturonic acid, 2-amino-2-deoxyglucose, and a 3-deoxyoctulosonic acid. The putative O-specific polysaccharide was composed mainly of 2-amino-2-deoxy-D-glucose, D-arabinose, and 6-deoxy-L-talose, but also contained an O-acetyl group and small proportions of rhamnose and 6-deoxy-3-O-methyltalose. Degradative and n.m.r. (1H and 13C) studies showed that the polymer had a branched trisaccharide repeating-unit with the following structure; the O-acetyl group was tentatively assigned to C-2 of the 6-deoxytalopyranosyl residue. (Formula: see text).

Lipid composition and taxonomy of (Pseudomonas) echinoides: transfer to the genus Sphingomonas

Lipid components of [Pseudomonas] echinoides NCIMB 9420 have been studied as an aid to taxonomic relocation of the organism. Non-polar lipids include the carotenoid nostoxanthin and the ubiquinone Q-10. The major fatty acids are cis-vaccenic acid [18:1(11c)], hexadecanoic acid (16:0) and 2-hydroxy-tetradecanoic acid (2-OH-14:0), but 11-methyloctadec-11-enoic acid[11-Me-18:1(11)] is a significant minor component. The preponderant phospholipids are phosphatidylethanolamine and phosphatidylglycerol; minor lipids include bis(phosphatidyl)glycerol and an unidentified aminophospholipid. Several glycolipids are present, the major one being a glucuronosylceramide derived from sphinganine with amide-bound 2-OH-14:0. The lipid profile supports a proposal to reclassify the organism as Sphingomonas echinoides.

Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii

Summary: The polar lipids and their fatty acid components in Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii have been identified. In addition to diphosphatidylglycerol and phosphatidylglycerol (a trace only for P. pickettii), all three species contained two forms of phosphatidylethanolamine differing in the presence or absence of α-hydroxy fatty acids. This seems to be a distinctive feature of species in Pseudomonas RNA homology group II. Also, P. caryophylli and P. gladioli (but not P. pickettii) produced two forms of ornithine amide lipid, differing in the nature (hydroxy or non-hydroxy) of the ester-linked fatty acid. In all three species, the major non-hydroxy acids were hexadecanoic acid, a hexadecenoic acid, an octadecenoic acid, and cyclopropane derivatives of the monoenoic acids. The α-hydroxy acids were the derivatives of the same components, while the amide-linked acid of the ornithine amide lipids was mainly or entirely 3-hydroxyhexadecanoic acid. The possible taxonomic implications of the data are discussed.

Polar lipids of Pseudomonas vesicularis. Presence of a heptosyldiacylglycerol.

The individual polar lipids produced by Psuedomonas vesicularis NCTC 10 900 during surface culture have been isolated. The major lipids are phosphatidylglycerol, a phosphatidyl-alpha-D-glucopyranosyldiacylglycerol, 1,2-di-O-acyl-3-O-alpha-D-glucopyranosylglycerol. 1,2-di-O-acyl-3-O-alpha-D-glucopyranuronosylglycerol, and 1,2-di-O-acyl-3-O[beta-D-glucopyranosyl-(1 yields 4)-alpha-D-glucopyranuronosyl]glycerol. These are also the major polar lipids of Pseudomonas diminuta. Additional lipids present in P. vesicularis are unidentified carotenoids and a novel lipid characterised as 1,2-di-O-acyl-3-O-alpha-D-glycero-D-glucoheptopyranosylglycerol. A cis-octadecenoic acid and hexadecanoic acid are the major fatty acids: C15 and C17 acids are significant minor components. The fatty methyl ester fractions derived from three of the lipids (most notably the glucosyldiacylglycerol) contained substantial amounts of a compound with chromatographic properties resembling those of an octadecenoic ester: the identity and origin or this compound remained uncertain.

Structure of a surface polysaccharide from Acinetobacter baumannii strain 214

A polysaccharide containing D-galactose, D-glucose, and 2-acetamido-2-deoxy-D-galactose was obtained from an aqueous phenol extract of isolated cell walls from Acinetobacter baumannii strain 214. By means of NMR studies and chemical degradations, the repeating unit of the polymer was identified as a branched trisaccharide of the structure shown. [formula: see text]

Structure of the O-specific polysaccharide for Acinetobacter baumannii serogroup O1.

A polymeric fraction containing D-galactose, N-acetyl-D-galactosamine, and N-acetyl-D-glucosamine was isolated from the lipopolysaccharide produced by the reference strain for Acinetobacter baumannii serogroup O1. By means of NMR spectroscopy, methylation analysis, and chemical degradation, the repeating unit of the polymer was identified as a branched trisaccharide of the following structure. [formula: see text].

Structure of the O19 antigen of xanthomonas maltophilia

The O-specific polymer from a strain of Xanthomonas maltophilia O19 contains D-glucose, L-rhamnose, and D-fucose. By means of chemical degradations and NMR studies, the repeating unit of the polymer was determined to be a branched tetrasaccharide of the structure shown. [formula: see text]

Structure of the O-specific polysaccharide from Enterobacter cloacae strain N.C.T.C. 11579 (serogroup O10)

The O-specific polysaccharide from the reference strain (N.C.T.C. 11579) for Enterobacter cloacae serogroup O10 has been isolated and characterised. By means of n.m.r. spectroscopy and methylation analysis, and by studies of the products obtained by Smith degradation or by N-deacetylation-deamination, the repeating unit of the polysaccharide could be allocated the structure shown. The polysaccharides from two cross-reacting serogroups (O9 and O11) have the same monosaccharide composition. (Formula: see text)

Structures of the O21 and O25 antigens of Stenotrophomonas maltophilia

The O-specific side-chain polymers from Stenotrophomonas maltophilia serogroups O21 and O25 were isolated from the lipopolysaccharides of the reference strains. The O21 polymer contained D-arabinose, 2-amino-2-deoxy-D-glucose and 2-amino-2-deoxy-D-galactose in equal proportions. Methylation analysis and NMR spectroscopy showed that the polysaccharide is based on a branched trisaccharide repeating unit of the structure shown below. The O25 polymer is linear with a disaccharide repeating unit identical to that forming the backbone of the O21 polymer.

Structural studies of the O-specific side-chain of lipopolysaccharide from Burkholderia gladioli pv. gladioli strain NCPPB 1891

A polymeric fraction (the O-antigenic side-chain) has been isolated from the lipopolysaccharide of Burkholderia gladioli pv. gladioli strain NCPPB 1891 after mild acid hydrolysis. The components of the polymer and their molar proportions were L-Rha (1), D-Gal (1), D-Man (1), and O-acetyl (1). By means of chemical degradations and NMR studies, the repeating unit of the polymer was shown to be a linear trisaccharide of the structure shown. [formula: see text]