Leticia García-Salguero
University of Granada
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Featured researches published by Leticia García-Salguero.
The International Journal of Biochemistry & Cell Biology | 1998
Marı́a J. Sánchez-Muros; L. García‐Rejón; Leticia García-Salguero; Manuel de laHiguera; José A. Lupiáñez
In fish, metabolic changes and qualitative responses during different nutritional situations are highly controversial in the scientific literature, and for this reason the objective of this work has been to probe deeper into the adaptive behaviour of two important amino acid-metabolising enzymes, glutamate dehydrogenase (GDH) and alanine aminotransferase (AAT) of liver and kidney in trout. In the present study, we examined the long-term effects of endogenous or exogenous proteins--generated, respectively, by a prolonged starvation or by feeding a high-protein diet--on the kinetics of liver and kidney GDH and AAT. Feeding on a high-protein diet significantly increased the liver (100%) and kidney (49%) GDH Vmax and catalytic efficiency; the same kinetic parameters of AAT increased by 65% only in the liver enzyme, without changing the Km and activity ratio values. Starvation registered a significant increase of both enzymes, Vmax and catalytic efficiency in the liver, but activity was unaltered in the kidney. In addition, no significant changes were found in the Km or activity ratio. All enzyme kinetics showed a Michaelian behaviour without any evidence of sigmoidicity. The experimental results show strong adaptive responses in the kinetic behaviour of the enzymes of both tissues. With the exception of renal AAT, the remainder of the enzymes presented a marked influence in their kinetic parameters by an excess of protein. The results are discussed in terms of the possible adaptive role of enzyme kinetics to amino acid availability.
Aquaculture | 1999
Juan Peragón; Juan B. Barroso; Leticia García-Salguero; Manuel de la Higuera; José A. Lupiáñez
Abstract We have investigated the effect of dietary carbohydrate on different parameters of protein-turnover rate, nature of growth, and nucleic acid content in the muscle of rainbow trout in order to better understand the molecular nature of these growth parameters in the absence of this dietary component. For this, we used a methodology based on the incorporation rate of tritium labelled phenylalanine in muscle protein. Juvenile rainbow trout of an initial body weight of 110 g were fed near to satiety with a control or a non-carbohydrate diet during 7 weeks. The absence of dietary carbohydrate significantly depressed fish growth, as well as daily body weight gain, as a consequence of muscular hypotrophy (the cell size diminished by almost 50%) and not by a reduction of number of cells (hypoplasia). This nutritional situation also significantly slowed (by almost 11%) muscle-protein accumulation rate ( K G ) as a result of a significant increase (eight-fold) in muscle-protein degradation rate ( K D ), without changing the other protein-turnover rates, protein synthesis rate ( K S ), protein synthesis capacity ( C S ), protein synthesis efficiency ( K RNA ), protein synthesis rate per cell unit ( K DNA ), or protein retention efficiency (PRE). These results, together with the nucleic acid content, clearly indicate that the absence of carbohydrate significantly exacerbates the muscular-protein degradation without affecting protein synthesis. In conclusion, carbohydrates are needed to prevent amino acids released during protein degradation from being used to synthesize carbohydrates and/or to be used for energy and not for growth.
American Journal of Physiology-regulatory Integrative and Comparative Physiology | 1998
Juan B. Barroso; Juan Peragón; Constanza Contreras-Jurado; Leticia García-Salguero; Francisco J. Corpas; Francisco J. Esteban; Maria Angeles Peinado; Manuel de la Higuera; José A. Lupiáñez
Herein we report on the kinetic and protein expression of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase, and malic enzyme (ME) in the liver of the trout (Oncorhynchus mykiss) during a long-term starvation-refeeding cycle. Starvation significantly depressed the activity of these enzymes by almost 60%, without changing the Michaelis constant. The time response to this nutritional stimulus increased with fish weight. The sharp decline in G6PDH and ME activities was due to a specific protein-repression phenomenon, as demonstrated by molecular and immunohistochemical analyses. Also, the dimeric banding pattern of liver G6PDH shifted from the fully reduced and partially oxidized forms, predominant in control, to a fully oxidized form, more sensitive to proteolytic inactivation. Refeeding caused opposite effects in both protein concentration and enzyme activities of about twice the control values in the first stages, later reaching the normal enzyme activity levels. Additionally, the partially oxidized form of G6PDH increased. The kinetics of these enzymes were examined in relation to the various metabolic roles of NADPH. These results clearly indicate that trout liver undergoes protein repression-induction processes under these two contrasting nutritional conditions.
The International Journal of Biochemistry & Cell Biology | 2001
Juan Peragón; Juan B. Barroso; Leticia García-Salguero; Manuel de la Higuera; José A. Lupiáñez
We have studied the growth rate, nucleic-acid concentration, protein-accumulation rate (K(G)), and several other parameters relating to protein turnover, such as the protein-synthesis (K(S)), and protein-degradation rates (K(D)), protein-synthesis capacity (C(S)), protein-synthesis efficiency (K(RNA)), protein-synthesis rate per DNA unit (K(DNA)) and protein-retention efficiency (PRE), in the white muscle of rainbow trout during development. Both growth rate and relative food intake decreased significantly with age and weight, as did the food-efficiency ratio (FER) and protein-efficiency ratio (PER). Although absolute RNA and DNA contents increased with age, their relative concentrations decreased. The RNA/DNA ratio increased sharply from 14 to 28 weeks but afterwards decreased towards initial values. Hypertrophy increased rapidly to the 28-week stage but henceforth increased much more slowly. Hyperplasia, on the other hand, continued to increase linearly, resulting in a significant four- to fivefold predominance in this type of growth at the end of the 96-week experimental period. K(G) decreased significantly with age, as did K(S), and C(S), whereas at the 14-week stage, K(D) was significantly lower than at other ages. K(RNA) increased until 28 weeks. K(DNA) increased significantly in juvenile fish compared to both fingerlings and adults, where it showed similar lower values. PRE remained high at all ages.
Life Sciences | 1994
F. Javier Corpas; Leticia García-Salguero; Juan Peragón; José A. Lupiáñez
Glucose-6-phosphate dehydrogenase (G6PDH) from rat-liver and kidney-cortex cytosol has been partially purified and almost completely separated from 6-phosphogluconate dehydrogenase activity. The purification and isolation procedures included high-speed centrifugation, 40-55% ammonium sulphate fractionation, by which both enzyme activities were separated, and finally, the application of the protein fraction to a column of Sephadex G-25 equilibrated with 10 mM Tris-EDTA-NADP buffer, pH 7.6, to eliminate any contaminating metabolites. The kinetic properties of isolated liver and renal G6PDH were examined. Both enzymes showed a typical Michaelis-Menten kinetic saturation curve with no evidence of co-operativity. The optimum pH of both liver and kidney cortex G6PDH was 9.4. The Km values for glucose-6-phosphate (G6P) and for NADP were 3.29 x 10(-4) M and 1.00 x 10(-4) M respectively. The specific activity measured at 37 degrees C and optimum pH was 327.1 mU/ mg of protein. NADPH caused a competitive inhibition with a Ki of 10 microM. The Km values for the G6P and NADP of kidney-cortex G6PDH were 2.06 x 10(-4) and 0.25 x 10(-4) M respectively. The specific activity at pH 9.4 and 37 degrees C was 76.55 mU/mg of protein. The Ki value for NADPH inhibition was 4 microM. This work describes an easy, rapid and reliable method for the separation of the two dehydrogenases involved in the hexose-monophosphate shunt in animal tissues.
Molecular and Cellular Biochemistry | 1999
Juan Peragón; Juan B. Barroso; Leticia García-Salguero; Fermín Aranda; Manuel de la Higuera; José A. Lupiáñez
We report upon the effects of a cycle of long-term starvation followed by re-feeding on the liver-protein turnover rates and nature of protein growth in the rainbow trout (Oncorhynchus mykiss). We determined the protein-turnover rate and its relationship with the nucleic-acid concentrations in the livers of juvenile trout starved for 70 days and then re-fed for 9 days. During starvation the total hepatic-protein and RNA contents decreased significantly and the absolute protein-synthesis rate (AS) also fell, whilst the fractional protein-synthesis rate (KS) remained unchanged and the fractional protein-degradation rate (KD) increased significantly. Total DNA content, an indicator of hyperplasia, and the protein:DNA ratio, an indicator of hypertrophy, both fell considerably. After re-feeding for 9 days the protein-accumulation rates (KG, AG) rose sharply, as did KS, AS, KD, protein-synthesis efficiency (KRNA) and the protein-synthesis rate/DNA unit (KDNA). The total hepatic protein and RNA contents increased but still remained below the control values. The protein:DNA and RNA:DNA ratios increased significantly compared to starved fish. These changes demonstrate the high response capacity of the protein-turnover rates in trout liver upon re-feeding after long-term starvation. Upon re-feeding hypertrophic growth increased considerably whilst hyperplasia remained at starvation levels.
Aquaculture | 1994
Juan Peragón; Juan B. Barroso; Leticia García-Salguero; Manuel de la Higuera; José A. Lupiáñez
Abstract We have studied the effects of a decrease in dietary protein on the growth and proteinturnover parameters [fractional protein synthesis ( K S ), degradation ( K D ) and accumulation ( K G ) rates, synthesis capacity ( C S ), synthesis efficiency ( K RNA and K DNA ), and protein retention efficiency (PRE)]. The administration of a low-protein diet caused a significant decrease in the availability of protein precursors due to a decline in feed efficiency and thus a concomitant decrease in whole-body, white-muscle and liver growth capacity throughout the experimental period. The reduction in dietary protein caused a significant decrease in the K G value in the liver as a consequence of a decrease in protein retention efficiency (PRE). In white muscle, however, a considerable reduction in the K G value was due to a decrease in K S , C S , K DNA and K RNA rather than a decrease in PRE.
Aquaculture | 1994
Juan B. Barroso; Leticia García-Salguero; Juan Peragón; Manuel de la Higuera; José A. Lupiáñez
Abstract The effects of a decrease in dietary protein level on the kinetic behaviour of the four NADPH production systems [glucose 6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), malic enzyme (ME), and NADP-isocitrate dehydrogenase (NADP-IDH)] were investigated in four different trout tissues. Typical hyperbolic saturation curves were always obtained for the activity of these enzymes. A low-protein diet caused a significant decrease in the maximum velocity values ( V max ) of all the hepatic enzyme systems studied. The decrease in hexose monophosphate dehydrogenases (G6PDH and 6PGDH) was about 35 and 50%, respectively, whereas for ME and NADP-IDH it was about 35 and 25%. No significant changes were found in the Michaelis constant. These kinetic characteristics are compatible with an increase in the catalytic efficiency of these enzymes without there being any changes in their activity ratio values. The kinetic parameters for these enzymes in the kidney, spleen, and gill tissue did not undergo any significant change.
Molecular and Cellular Biochemistry | 2000
Juan Peragén; Juan B. Barroso; Leticia García-Salguero; Manuel de la Higuera; José A. Lupiáñez
We have determined the protein-turnover rates and nucleic-acid concentrations in the liver of trout (Oncorhynchus mykiss) fed on two different isocaloric diets: low-protein/high-fat and non-carbohydrate/high-fat. Compared to controls, the partial replacement of protein with fat significantly decreased the protein accumulation rate and protein-retention efficiency in the liver whilst increasing the fractional protein-synthesis and protein-degradation rates as well as protein-synthesis efficiency. The complete replacement of carbohydrates with fat significantly lowered the protein-accumulation rate and protein-retention efficiency, but enhanced both the protein-synthesis and protein-degradation rates as well as protein-synthesis capacity. The protein:DNA and RNA:DNA ratios decreased considerably on both diets. Total DNA decreased in fish on a low-protein/high-fat diet but did not change in those on a non-carbohydrate/high-fat diet. The absolute protein-synthesis rate registered no significant change under any of the nutritional conditions. Both the experimental diets did however raise the fractional protein-synthesis rate significantly, due to enhanced protein-synthesis efficiency when protein was partially replaced with fat and to enhanced protein-synthesis capacity when carbohydrates were completely replaced with fat. Our results show the capacity of the liver to adapt its turnover rates and conform to different nutritional conditions. They also point to the possibility of controlling fish growth by dietary means.
Molecular and Cellular Biochemistry | 1989
Leticia García-Salguero; José A. Lupiáñez
SummaryThe influence of starvation on renal carbohydrate metabolism was studied in the proximal and distal fragments of the nephron. Starvation induced a double and opposite adaptation mechanism in both fractions of the renal tubule. In renal proximal tubules, the gluconeogenic flux was stimulated progressively during a period of 48 hours of starvation (2.15 fold), due, in part, to a significant increase in the fructose 1,6-bisphosphatase and phosphoenolpyruvate carboxykinase activities although with different characteristics. Fructose 1,6-bisphosphatase activity from this tubular fragment increased only at subsaturating subtrate concentration (68%) which involved a significant decrease in the Km (35%) for fructose 1,6-bisphosphate while there was no change in Vmax. This behaviour clearly indicates that it is related to modifications in the activity of the preexistent enzyme in the cell. Proximal phosphoenolpyruvate carboxykinase activity increased proportionally at both substrate concentrations (86 and 89% respectively) which brought about changes in Vmax without changes in Kin, all of which are in accordance with variations in the cellular levels of the enzyme. In the renal distal tubules, the glycolytic capacity drastically decreased throughout the starvation time. At 48 hours 65% of inhibition was shown. We have found a short term regulation of phosphofructokinase activity by starvation which involves an increase in Km (2.2 fold) without changes in Vmax, as a result of these kinetic changes, an inactivation of phosphofructokinase was detected at subsaturating concentration of fructose 6-phosphate. On the contrary, this nutritional state did not modify the kinetic behaviour of renal pyruvate kinase. Finally, neither proximal glycolytic nor distal gluconeogenic capacities and related enzymes activities were changed during starvation.