Letizia Garibaldi

Trophic development of the deep lakes south of the Alps: a comparative analysis

This paper reports the results of long-term research carried out in the deep southern subalpine lakes (DSL: Maggiore, Lugano, Como, Iseo and Garda). The measurements performed since the 1970s at the time of the maximum overturn documented an increase of water temperature around 0.012-0.028 °C yc -1 , closely resembling the warming rate found in other lakes in Europe and North America. The DSL showed an increase of total phosphorus after the 1960s and a complete recovery after the 1990s of Lake Maggiore, which reached mean values of TP close to those of the pristine conditions (<10μgPl -1 Present TP concentrations in the other lakes are above 100 μg P l -1 (Lugano), 30-40 μg P l -1 (Como), 50-60 μg P l -1 (Iseo) and c. 20 μg P l -1 (Garda), with only Lake Garda showing a clear tendency to increasing P-concentrations in recent years. The higher trophic status of lakes Lugano and Iseo has favoured hypolimnetic anoxia due to meromixis since the 1960s and the 1990s, respectively, from which there was a partial recovery in 2005. Monthly samplings carried out in the last decades showed a strong coupling between long-term variation in algal nutrients and phytoplankton. On a shorter temporal scale, a significant relationship between the extent of water mixing during the spring months and the replenishment of nutrients and algal biomass in the growing season has also been verified in lakes Iseo, Garda and Lugano.

Phytoplankton as an Indicator of the Water Quality of the Deep Lakes South of the Alps

This paper offers a synoptic account of studies on the phytoplankton communities in the deep southern subalpine lakes (DSL) Garda, Iseo, Como, Lugano and Maggiore. The main cause of the degradation of the water quality in the DSL is eutrophication. The euphotic layers of these lakes are trophically different, ranging from the oligo-mesotrophy of lakes Maggiore and Garda to the meso-eutrophy of lakes Iseo and Lugano. The trophic status as estimated by using total phosphorus and chlorophyll a has provided consistent results in agreement with the models proposed by OECD (1982. Eutrophication of Waters. Monitoring, Assessment and Control, OECD, Paris). Though related with chlorophyll a and TP, the Secchi disk depths have significantly underestimated the trophic status of the DSL. Two trophic indices using the algal orders (PTIorders) and species (PTIspecies) were drawn up on the basis of the distribution of phytoplankton along a trophic gradient defined by the application of multivariate methods; the scores emerging from these indices were used to make a definitive ecological classification of water bodies on a scale from 1 to 5, in accordance with the Water Framework Directive. A third index (PTIOE) was computed as the ratio between the annual mean values of the cumulative biovolumes of two groups of algal orders with opposite trophic characteristics. The three PTI indices were highly correlated, providing a consistent classification of the water bodies. The indices proposed in this work were specifically adopted for use in the DSL. However, the criteria for their implementation constitute a robust and impartial tool for assessing similar indices in other lake typologies and for evaluating the degree of specificity of the trophic indicator values assigned to the single phytoplankton orders and species.

Effects of nutrient availability and temperature on phytoplankton development: a case study from large lakes south of the Alps

This work investigated the combined effects of nutrient availability and temperature on phytoplankton in large and deep lakes south of the Alps (lakes Garda, Iseo, Como, Lugano and Maggiore). The more eutrophic basins (Lugano and Iseo) showed a higher presence of cyanobacteria, green algae (Chlorophyta and Charophyta) and dinoflagellates (Dinophyta). Besides these two water bodies, high biomasses of cyanobacteria were recorded also in the oligo-mesotrophic Lake Garda. The development of these algal groups during the growing season showed a strong dependence on the surface spring availability of SRP, which, in turn, was related to winter climatic oscillations, deep mixing dynamics, and trophic status. A specific analysis carried out by applying additive mixed modelling, generalized least squares and mixed modelling, allowed investigation of the direct, seasonal effects of water temperature variations and trophic status on different algal groups. The dominant cyanobacteria (Oscillatoriales) showed only a partial relationship with temperature, while Nostocales and Chroococcales, which did not appear to have a close relationship with the trophic status of the lakes, were characterised by abrupt increases during the warmer months. High positive relationships with temperature were found for a few other algal groups (e.g., Chlorophyta, Charophyta and Dinophyta). Overall, the results indicated a positive relationship between the seasonal development of the more abundant and eutrophic-sensitive algal groups and the concurrent effect of trophic status and water temperature. Nevertheless, it was stressed that specific differences could be interpreted taking into account the different autoecological characteristics and susceptibilities of different species and functional groups to other stressing factors favouring losses, including, e.g., vertical sinking and grazing.

Influence of atmospheric modes of variability on the limnological characteristics of large lakes south of the Alps: a new emerging paradigm

Winter air and spring surface water temperatures, spring epilimnetic phosphorus, and hypolimnetic oxygen in the deep lakes south of the Alps (Garda, Iseo, Como, Lugano, and Maggiore) showed a high degree of temporal coherence. The common temporal patterns were originating from the effects caused by winter climate, and from corresponding synchronisms in the interannual variations in the extent of the spring water renewal and replenishment of nutrients. In turn, the sequence of linked causal events was triggered by two atmospheric modes of variability relevant for the Mediterranean region, i.e. the East Atlantic pattern (EA) and the Eastern Mediterranean Pattern (EMP). In contrast, there were no significant relationships of air and water temperatures with the North Atlantic Oscillation. In oligotrophic lakes, which were characterised by weak vertical nutrient gradients, the spring replenishment of P was negligible and difficult to detect (Maggiore), or detectable but of minor importance (Garda and Como) compared to that measured in more enriched lakes (Iseo and Lugano). The applicability of EA and EMP in the study of the impact of climate on aquatic ecosystems will require to be tested by expanding the number, typology, and geographical location of water bodies in the Alpine and Mediterranean regions.

Response of planktonic communities to calcium hydroxide addition in a hardwater eutrophic lake: results from a mesocosm experiment

The main focus of this study was to investigate the effects of lime treatment with Ca(OH)2 on a hypereutrophic hardwater lake (Lake Alserio, Northern Italy), focusing on its impact on plankton communities. We performed a mesocosm experiment using two large enclosures, one for treatment and one control, in the lake. The addition of Ca(OH)2 proved to be effective in reducing phosphorus concentration, but the effect was limited in time. Redissolution of the precipitating calcium carbonate as it came into contact with the deep water determined a phosphorus increase about 2 weeks after the liming. The liming had a strong impact on the plankton assemblage, as was demonstrated by the sharp decrease in phytoplankton and zooplankton biomass and density, and 1 month after the treatment we observed a different taxonomic composition inside the enclosure from that of the lake. The result of the experiment showed that liming with Ca(OH)2 can be regarded as a suitable treatment for accelerating recovery of the lake. Multiple treatments will be necessary to improve the trophic level over a longer period.

Summer spatial variations in phytoplankton composition and biomass in surface waters of a warm-temperate, deep, oligo-holomictic lake: Lake Iseo, Italy

Abstract The spatial distribution of phytoplankton in the surface waters of a typical, deep subalpine lake was investigated by analysing water samples collected at 3 depth ranges (0–1, 9–13, and 15–19 m) at 7 sites (21 total samples) using a generalized linear model. The biovolume of different phytoplanktonic taxa significantly differed between depth ranges. Different algal taxa showed maximum population density at different depths as a result of different optima of light, physical conditions, and nutrients. The layer at 9–13 m depth showed the greatest abundance of phytoplankton, mainly represented by Bacillariophyceae and Cyanobacteria. Conversely, Chlorophyceae, which require few nutrients and are not inhibited by high light energy, were most abundant at the surface. The vertical heterogeneity in phytoplankton distribution closely paralleled that of nutrients, which in turn depend on the stable thermal stratification of the lake water column. Biovolume of phytoplankton taxa and chemical parameters did not differ significantly between sampling sites some kilometers apart due to large horizontal water movements in the lake that create relatively uniform conditions throughout the basin.

How does interannual trophic variability caused by vertical water mixing affect reproduction and population density of the Daphnia longispina group in Lake Iseo, a deep stratified lake in Italy?

Abstract Lake Iseo is a deep meromictic lake located in Italy. During the past 20 years (1993–2013), the lake has experienced complete mixing of the water column only in spring 2005 and 2006. The full overturn episodes in these 2 years resulted in an increase in nutrients in both years, but an increase in phytoplankton biovolume occurred in the surface layers only in 2005. Our study examined if the magnitude of vertical mixing and the resulting increase in concentrations of epilimnetic total phosphorous (TP) can help predict reproduction and abundance of the main primary consumers, the cladocerans. We investigated the lake for 9 years (2001–2009) and compared annual changes in the Cladocera community and reproduction of the Daphnia longispina group between years of full and partial mixing. During the complete vertical mixing years (2005 and 2006), the taxonomical composition of Cladocera did not change, and density relationships among taxa shifted slightly toward an increase in the population density of Daphnia spp. Phytoplankton biovolume was significantly related to Daphnia mean clutch size. The increase in TP subsequent to the complete vertical mixing during late winter–early spring also seemed to predict reproduction and population density of Daphnia species. To our knowledge, this is the first long-term field study highlighting the importance of vertical mixing depth and TP concentrations on Daphnia abundance and phenology in a meromictic–oligomictic deep lake.

Using time scales to characterize phytoplankton assemblages in a deep subalpine lake during the thermal stratification period: Lake Iseo, Italy

A combination of field observations and 3-D hydrodynamic simulations were used to identify the phytoplankton species and to estimate the various time scales of the dominant physical and biological processes in Lake Iseo, a deep subalpine lake located in northern Italy, during a stratified period (July 2010). By ordering the rate processes time scales, we derive a phytoplankton patch categorization and growth interpretation that provides a general framework for the spatial distribution of phytoplankton concentration in Lake Iseo and illuminates the characteristics of their ecological niches. The results show that the diurnal surface layer was well mixed, received strong diurnal radiation, had low phosphorus concentrations and the phytoplankton biomass was sustained by the green alga Sphaerocystis schroeterii. The vertical mixing time scales were much shorter than horizontal mixing time scales causing a depth-uniform chlorophyll a concentration. The horizontal patch scale was determined by horizontal dispersion balancing the phytoplankton growth time scale, dictating the success of the observed green algae. The strongly stratified nutrient-rich metalimnion had mild light conditions and Diatoma elongatum and Planktothrix rubescens made up the largest proportions of the total phytoplankton biomass at the intermediate and deeper metalimnetic layers. The vertical transport time scales were much shorter than horizontal transport and vertical dispersion leading to growth niche for the observed phytoplankton. The study showed that time-scale hierarchy mandates the essential phytoplankton attributes or traits for success in a particular section of the water column and/or water body.