Lia Magalhães de Almeida

Immunological response in mice immunised via oral route with açaí (Euterpe oleracea Mart.)

The açaí berry (Euterpe oleracea Mart.) is a typical fruit of the Amazon region. The consumption of the açaí pulp has grown in both the national and international markets due to its high nutritional and energetic value. The pulp is also rich in phenolic compounds, mainly anthocyanin, giving it antioxidant properties and thereby also the status of being a functional food. This study aims to evaluate the immunological response in mice immunised via oral route with lyophilised açaí pulp (LAP). Swiss mice were immunised by oral route with 200 µg of LAP. Specific antibodies (IgE and IgG1) were detected in the serum through the methods enzyme linked immunosorbent assay (ELISA) and immunoblotting with immunoprecipitation. The results therefore suggest that the açaí berry can be considered a food allergen. Further studies are required to elucidate the immunological mechanisms involved in the allergic reaction to açaí.

Humoral immune response in mice immunised by oral and subcutaneous routes with Isosoy proteins

Abstract This study compares the allergenic potential of Isosoy®, a soy germen commercialised as isoflavone for dietary supplements, with crude soy extract (CSE) and purified isoflavone. Specific antibodies (IgG, IgE and IgG1) generated in Swiss mice by oral and subcutaneous route with Isosoy® also recognised proteins of crude soy extract, but no reaction against purified isoflavone, like clearly demonstrated by ELISA, Western blot and immunoprecipitation assay. These results are in agreement with the same protein profile between Isosoy® and CSE observed by polyacrylamide gel electrophoresis (SDS-PAGE). The immunoprecipitation assay demonstrates that Isosoy® boiling cannot inactivate the interaction with specific antibodies generated by oral route with unboiled Isosoy®. Results suggest that Isosoy® could lead to allergic reactions even after heat treatment.

Experimental model of type 2 diabetes induced by fat diet consumption and low dose of streptozotocin in C57BL/6J mice

Experimental model of type 2 diabetes induced by fat diet consumption and low dose of streptozotocin in C57BL/6J mice Mariana de Freitas Moreira, Natalia do Vale Canabrava, Sandra Machado Lira, Tatiane Rodrigues Oliveira, Jose Ytalo Gomes da Silva, Marcelo Oliveira Holanda, Carla Laine Silva Lima, Julianne do Nascimento Sales, Arnaldo Solheiro Bezerra, Bruno Bezerra da Silva, Erlândia Alves Magalhaes Queiroz, Paula Alves Salmito Rodrigues, Raquel Teixeira Terceiro Paim, Lia Magalhaes de Almeida, Maria Izabel Florindo Guedes

Evaluation of the acute toxicity oral of carnauba powder (PCO-C) in mice C57BL/6

Evaluation of the acute toxicity oral of carnauba powder (PCO-C) in mice C57BL/6 Paula Alves Salmito Rodrigues, Jose Ytalo Gomes da Silva, Marcelo Oliveira Holanda, Carla Laine Silva Lima, Raquel Teixeira Terceiro Paim, Sandra Machado Lira, Natalia do Vale Canabrava, Mariana de Freitas Moreira, Juliana Barbosa Dantas, Thais Vital de Freitas, Julianne do Nascimento Sales, Bruno Bezerra da Silva, Erlândia Alves Magalhaes Queiroz, Chayane Gomes Marques, Lia Magalhaes de Almeida, Icaro Gusmao Pinto Vieira, Francisca Noelia Pereira Mendes, Rafaela Valesca Rocha Bezerra Sousa, Arnaldo Solheiro Bezerra, Maria Izabel Florindo Guedes

Evaluation of acute oral toxicity of red alga (Gracilaria domingensis sonder ex kützing) in mice C57BL/6

Evaluation of acute oral toxicity of red alga (Gracilaria domingensis sonder ex kutzing) in mice C57BL/6 Jose Ytalo Gomes da Silva, Marcelo Oliveira Holanda, Carla Laine Silva Lima, Paula Alves Salmito Rodrigues, Erlândia Alves Magalhaes Queiroz, Raquel Teixeira Terceiro Paim, Thais Vital de Freitas, Juliana Barbosa Dantas, Sandra Machado Lira, Natalia do Vale Canabrava, Mariana de Freitas Moreira, Bruno Bezerra Silva, Chayane Gomes Marques, Julianne do Nascimento Sales, Arnaldo Solheiro Bezerra, Emanuele Silva de Sousa, Rafaela Valesca Rocha Bezerra Sousa, Lia Magalhaes de Almeida, Francisca Noelia Pereira Mendes, Icaro Gusmao Pinto Vieira, Maria Izabel Florindo Guedes

Production of dengue 2 envelope domain III in plant using CPMV - based vector system

Background The envelope protein (E protein) of dengue virus is responsible for binding the virus to the host cell. This protein is considered an important immunogen for neutralization of the virus, the only able to induce the production of neutralizing antibodies [1]. The aim of this study was to use the Cowpea Mosaic Virus (CPMV) as a vector to express, on cowpea plants, Vigna unguiculata L. (Walp) the gene fragment that encodes for domain III of the E protein of dengue 2 virus.Background The envelope protein (E protein) of dengue virus is responsible for binding the virus to the host cell. This protein is considered an important immunogen for neutralization of the virus, the only able to induce the production of neutralizing antibodies [1]. The aim of this study was to use the Cowpea Mosaic Virus (CPMV) as a vector to express, on cowpea plants, Vigna unguiculata L. (Walp) the gene fragment that encodes for domain III of the E protein of dengue 2 virus.

Cloning and expression of the NS1 protein of dengue virus in a prokaryotic system

Background Dengue virus nonstructural protein 1 (NS1) is a highly conserved glycoprotein involved in the production of infectious virus and the pathogenesis of dengue disease [1]. Serum or plasma DENV NS1 level has been found to correlate with viremia titer and disease severity. It can be found in the peripheral blood circulation for up to 9 days from illness onset, but can persist for up to 18 days from illness onset in some cases. Thus NS1 detection offers a larger window of opportunity for diagnosis of dengue compared with virus isolation, RT-PCR or NASBA [2]. In this context, the aim of this work has been the establishment of conditions for expression and purification of the recombinant protein NS1 of dengue virus serotype 2 produced in E. coli for further development of a serological diagnostic method at low cost.