Liangwei Qu

Advances toward bioapplications of carbon nanotubes

Bioapplications of carbon nanotubes have been predicted and explored ever since the discovery of these one-dimensional carbon allotropes. Indeed, carbon nanotubes have many interesting and unique properties potentially useful in a variety of biological and biomedical systems and devices. Significant progress has been made in the effort to overcome some of the fundamental and technical barriers toward bioapplications, especially on issues concerning the aqueous solubility and biocompatibility of carbon nanotubes and on the design and fabrication of prototype biosensors. In this article we take a comprehensive look at the advances in this fast-moving and exciting research field. We review the current status of available methodologies for the aqueous dispersion and solubilization of carbon nanotubes, discuss the results on modifications of carbon nanotubes with various biological and bioactive species, and highlight some of the recent achievements in the fabrication and evaluation of carbon nanotube-based bioanalytical devices.

Acute and long-term effects after single loading of functionalized multi-walled carbon nanotubes into zebrafish (Danio rerio)

Carbon nanotubes (CNTs) are widely explored for biomedical applications, but there is very limited information regarding their in vivo biodistribution and biocompatibility. Here, we report the in vivo biodistribution and long-term effects of functionalized multi-walled carbon nanotubes (MWCNTs) in developing zebrafish. The fluorescent-labeled MWCNTs were introduced into zebrafish embryos at 1-cell stage and at 72 h post fertilization through microinjection. After single injection, both acute and long-term interactions between zebrafish and functionalized MWCNTs were studied. The injected FITC-BSA-MWCNTs (at 1-cell stage) were allocated to all blastoderm cells of the embryos through proliferation, and were distinctively excluded from the yolk cell. When introduced into the circulation system, FITC-BSA-MWCNTs moved easily in the compartments and finally were cleaned out by the body at 96 h after the loading. At early stages, the treated zebrafish embryos generated immune response by accumulating circulating white blood cells at the trunk region. Under transmission electron microscope, many lysosome-like vesicles were observed in the blastoderm cells of the treated embryos. The zebrafish loaded with MWCNTs had normal primordial germ cells at early stage and produced second generation later on. However, the larvae of the second generation had obviously lower survival rates as compared to the untreated groups, suggesting a negative effect on the reproduction potential. These results suggest that extensive purification and functionalization processes can help improve the biocompatibility of CNTs. This study also indicates that purified CNTs may have long-term toxicity effects when they were delivered into the body.

Interactions of functionalized carbon nanotubes with tethered pyrenes in solution

Single-wall carbon nanotubes (SWNTs) were functionalized by oligomeric species containing derivatized pyrenes. Absorption and emission properties of the pyrene moieties tethered to the functionalized SWNTs were studied in homogeneous solution. The absorption spectra suggest no significant ground-state complexation between the pyrenes and nanotubes. The fluorescence and fluorescence excitation results show that the tethered pyrenes form “intramolecular” (intra-nanotube) excimers and that the excimer formation is predominantly dynamic in nature. The time-resolved fluorescence results show that the pyrene monomer and excimer emissions are significantly quenched by the attached SWNTs. The quenching is explained in terms of a mechanism in which carbon nanotubes serve as acceptors for excited-state energy transfers from the tethered pyrene moieties.

Single-walled carbon nanotubes displaying multivalent ligands for capturing pathogens

A single-walled carbon nanotube was exploited for its semi-flexible pseudo-one-dimensional nanostructure as a unique scaffold to display multivalent carbohydrate ligands, with a specific demonstration showing that galactosylated carbon nanotubes were effective in the capturing of pathogenic Escherichia coli in solution.

Enhancing antimicrobial activity of lysozyme against Listeria monocytogenes using immunonanoparticles.

The antimicrobial efficacy of lysozyme may be reduced by undesirable interactions with food components and nontarget bacteria. Immunonanoparticles, i.e., nanoparticles functionalized with pathogen-specific antibodies, may serve as an antimicrobial carrier for improving the stability and activity of antimicrobials in foods. The objective of this research was to study the antimicrobial activity of lysozyme-carrying immunonanoparticles against Listeria monocytogenes. Polystyrene nanoparticles with active carboxyl groups were conjugated with anti-L. monocytogenes antibody through covalent bonding. Enhanced antimicrobial activity of lysozyme-carrying immunonanoparticles was achieved when 0.04 microg/ml anti-L. monocytogenes antibody was used for coating nanoparticles and the resulting immunonanoparticles were then coated with lysozyme for 6 h. Lysozyme-carrying immunonanoparticles with a final concentration of 35 microg/ml reduced L. monocytogenes Scott A populations from ca. 5 log CFU/ml to below the detection limit (< 1 log CFU/ml) within 3 h. However, when 500 microg/ml lysozyme was used, ca. 2 log CFU/ml concentration of L. monocytogenes cells remained culturable after 5 h of treatment. The addition of lysozyme-carrying immunonanoparticles (37 microg/ml) to an L. monocytogenes solution of ca. 7 log CFU/ml for 6 h resulted in 0.9-, 1.0-, and 2.3-log greater reductions of L. monocytogenes cells than that achieved with lysozyme-carrying nanoparticles and lysozyme solutions of 500 and 50 microg/ml, respectively. Overall, lysozyme-carrying immunonanoparticles had significantly more anti-L. monocytogenes activity (P < 0.05) than did lysozyme-carrying nanoparticles and lysozyme solutions at higher concentrations (500 and 50 microg/ml). Our study revealed that the use of lysozyme-carrying immunonanoparticles is more effective than direct addition of lysozyme for inactivating L. monocytogenes in nutrient broth.