Liangwen Xu
Hangzhou Normal University
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Featured researches published by Liangwen Xu.
PLOS ONE | 2015
Xiaohua Tan; Yuan Gao; Yulong Nan; Jinxia Zhang; Chunhong Di; Xiaobo Wang; Fuzhi Lian; Yifei Cao; Yu Hu; Liangwen Xu; Haiyan Ma; Yu Hong; Tingjie Liu; Yinyin Wu; Xianrong Xu; Yutao Yan; Lei Yang
Background Kaposi’s sarcoma (KS)-associated herpesvirus (KSHV) is the etiologic agent of KS, the most common AIDS-related malignancy. The majority of KS tumor cells harbor latent KSHV virus but only a small percentage undergoes spontaneous lytic replication. Viral reactivation from latency is crucial for the pathogenesis and development of KS, but the cellular mechanisms underlying the switch between viral latency and replication are not well understood. Methods The level of let-7 miRNAs and MAP4K4 in KSHV infected 293T cells were quantified by real-time PCRs. Let-7 expression was silenced by the miRNA sponge technique. In let-7a transfected 293T cells, the expression of MAP4K4 was measured by real-time PCR and western blot. Luciferease expression was employed to examine the effect of let-7a on the 3’-untranslated region (UTR) of the MAP4K4 gene in 293T cells. Real-time PCR was used to quantify the KSHV copy numbers in BC-3 cells in which the expression of let-7a and/or MAP4K4 were altered. Finally, ERK, JNK and p38 protein production and their phosphorylation status were detected by western blots in let-7a or MAP4K4 transfected BCBL-1 cells. Results The expression of microRNA let-7 was dramatically decreased in KSHV infected 293T cells, but that of MAP4K4 was increased significantly. Let-7a is physically associated with and targets the MAP4K4 3’UTR, and inhibits MAP4K4 expression at both mRNA and protein levels. MAP4K4 stimulates KSHV reactivation from latency, whereas let-7a inhibits the function of MAP4K4 by reversing the function of MAP4K4 on JNK, phospho-JNK and phospho-ERK1/2 levels. Conclusion Our results establish that let-7a specifically suppresses MAP4K4 expression, and further inhibits KSHV reactivation by interfering with the function of MAP4K4 on the MAPK pathway, highlighting let-7a as a potential treatment for KS.
Digestive Diseases and Sciences | 2016
Yanhu Li; Chunhong Di; Wen Li; Weibin Cai; Xiaohua Tan; Liangwen Xu; Lei Yang; Guoqiang Lou; Yutao Yan
Advances in Bioscience and Biotechnology | 2015
Chengtan Li; Xiayu Wang; Ya’ni Chen; Xiaohua Tan; Wen Li; Sheng Yan; Weibin Cai; Xianrong Xu; Liangwen Xu; Lei Yang; Yutao Yan
Indian Journal of Pediatrics | 2012
Chenjia Mao; Liangwen Xu; Li Xu; Haiyan Ma; Tingjie Liu; Xuping Qu; Hanqiong Hu; Qifa Yang
Chinese journal of epidemiology | 2011
Liangwen Xu; Xuping Qu; Mao Cj; Haiyan Ma; Tingjie Liu; Hu Hq; Yang Qf; Xu Lw
Chinese journal of preventive medicine | 2004
Liangwen Xu; Ma Hy; Yang Tz; Liu Tj
Brazilian Journal of Medical and Biological Research | 2018
Y.H. Li; Y. Yang; Yutao Yan; Liangwen Xu; Haiyan Ma; Y.X. Shao; C.J. Cao; X. Wu; M.J. Qi; Yinyin Wu; R. Chen; Y. Hong; Xiaohua Tan; Lei Yang
Environmental Health | 2017
Yinyin Wu; Juntao Ni; Mingjian Qi; Chengjian Cao; Yuxian Shao; Liangwen Xu; Haiyan Ma; Lei Yang
Archive | 2016
Yifei Cao; Chengtan Li; Wen Li; Yani Chen; Weibin Cai; Xiaohua Tan; Liangwen Xu; Lei Yang; Yutao Yan
Chinese journal of preventive medicine | 2015
Mingzhu Fang; Liangwen Xu; Xianhong Huang; Gu F; Xuping Qu; Xu M; Luo X