Lidia Nuñez
University of Buenos Aires
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Featured researches published by Lidia Nuñez.
Brazilian Journal of Microbiology | 2001
Lidia Nuñez; Miguel D'Aquino; Jorge Chirife
No estudo avaliou-se o efeito da temperatura, concentracao e tempo de contato na atividade antifungica do oleo essencial do cravo disperso em solucao concentrada de acucar. Os ensaios foram feitos a 21oC e a 37oC, utilizando suspensao de oleo essencial de 0.2 a 0.8% v/v. Os microorganismos utilizados foram Candida albicans, Penicillium citrinum, Aspergillus niger e Trichophyton mentagrophytes. A melhor atividade fungicida ocorreu a 37oC, onde um minuto de contato foi suficiente para matar uma populacao de 106 u.f.c./ml de Candida albicans. Nao obstante, P. citrinum, A. niger, T. mentagrophytes foram mais resistentes, embora uma importante acao letal da essencia tenha sido observada. Em 60 minutos, o oleo essencial de cravo (0,4% v.v.) em solucao concentrada de acucar reduziu em 99,6% um inoculo de 106 u.f.c./ml de T. mentagrophytes. O efeito letal do oleo essencial de cravo sobre C. albicans, apos 2 minutos de contato, foi semelhante ao de desinfectantes comumente usados em hospitais, como povidona-iodo e cloroxilenol.
Brazilian Journal of Microbiology | 2007
Lidia Nuñez; J. Moretton
Large quantities of disinfectants are used in hospitals, externally on human skin or to eliminate microorganisms from inanimate objects. After use, residual quantities of these products reach the wastewater, exposing the bacteria that survive in hospital wastewaters to a wide range of biocides that could act as a selective pressure for the development of resistance. Increasing attention has been directed recently to the resistance of bacteria to disinfectants. The aim of this paper was to determine the disinfectant bacterial resistance pattern of the microflora released to the urban sewer system by hospital effluents. The characterization of the waste water microflora was performed by determination of the CFU of heterotrophic bacteria, fecal indicator bacteria, Pseudomonas sp. and Staphylococcus sp., in a Buenos Aires hospital effluent. The bacterial resistance to the disinfectants more frequently used in the hospital practice, glutaraldehyde, chlorhexidine and povidone-iodine, was then evaluated. Disinfectant resistant bacterial strains were isolated and typified. Between 103 and 106 chlorexidine resistant bacteria/100 mL were isolated from the samples. Bacteria resistant to other disinfectants ranged between 103 and 104 /100 mL. The bacterial population resistant to desinfectants to was mainly composed by Enterobacteriaceae, Staphylococcus spp, and Bacillus spp, which are highly associated to nosocomial infections. The results obtained show that the hospital effluents are of importance in the bacterial resistance selection process, particularly in the case of disinfectants.
Brazilian Journal of Microbiology | 2012
Lidia Nuñez; M. D' Aquino
Clove essential oil, used as an antiseptic in oral infections, inhibits Gram-negative and Gram-positive bacteria as well as yeast. The influence of clove essential oil concentration, temperature and organic matter, in the antimicrobial activity of clove essential oil, was studied in this paper, through the determination of bacterial death kinetics. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were the microorganisms selected for a biological test. To determine the temperature effect, they were assayed at 21° and 37° C. The concentration coefficient was determined with 0.4%, and 0.2% of essential oil. The influence of the presence of organic matter was determined with 0.4% of essential oil. The results obtained demonstrated that Escherichia coli were more sensitive even though the essential oil exerted a satisfactory action in three cases. In the three microbial species, 0.4% of essential oil at 21° C have reduced the bacterial population in 5 logarithmic orders. Organic matter reduces the antibacterial activity even though the bactericide efficacy was not lost. Clove essential oil can be considered as a potential antimicrobial agent for external use
Zentralblatt Fur Bakteriologie-international Journal of Medical Microbiology Virology Parasitology and Infectious Diseases | 1990
Lidia Nuñez; Ethel Amato de Lagarde; Miguel D'Aquino
A counterimmunoelectrophoretic (CIE) technique was developed to detect botulinic toxin type A, and the method was compared with the mouse bioassay. A 100 LD50 concentration was detected within 2 h. Crossed reactions were observed with antitoxins of types B and F. As regards other Clostridium species, precipitin lines were seen between C. sporogenes and antitoxin type A. Foodstuffs artificially contaminated with C. botulinum type A were assayed by means of CIE and mouse bioassay, without recording interference by substances normally contained in foodstuffs.
Rev. argent. infectol | 1997
Miguel D'Aquino; Lidia Nuñez
Revista Ambiente & Água | 2012
Lidia Nuñez; Carina Tornello; Noel Puentes; Juan Moretton
Ambiente E Agua - An Interdisciplinary Journal of Applied Science | 2012
Lidia Nuñez; Carina Tornello; Noel Puentes; Juan Moretton
Ars Pharmaceutica | 2016
Lidia Nuñez; Carina Tornello; Noel Puentes; Elena Espigares; Elena Moreno; Miguel Espigares; Juan Moretton
Revista Internacional De Contaminacion Ambiental | 2014
Lidia Nuñez; Claudia Molinari; Marta Paz; Carina Tornello; Julián Mantovano; Juan Moretton
Acta toxicológica argentina | 2012
Anahí Magdaleno; Ángela Beatriz Juárez; Marta Paz; Carina Tornello; Lidia Nuñez; Juan Moretton