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Dive into the research topics where Lidia Nuñez is active.

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Featured researches published by Lidia Nuñez.


Brazilian Journal of Microbiology | 2001

Antifungal properties of clove oil (Eugenia caryophylata) in sugar solution

Lidia Nuñez; Miguel D'Aquino; Jorge Chirife

No estudo avaliou-se o efeito da temperatura, concentracao e tempo de contato na atividade antifungica do oleo essencial do cravo disperso em solucao concentrada de acucar. Os ensaios foram feitos a 21oC e a 37oC, utilizando suspensao de oleo essencial de 0.2 a 0.8% v/v. Os microorganismos utilizados foram Candida albicans, Penicillium citrinum, Aspergillus niger e Trichophyton mentagrophytes. A melhor atividade fungicida ocorreu a 37oC, onde um minuto de contato foi suficiente para matar uma populacao de 106 u.f.c./ml de Candida albicans. Nao obstante, P. citrinum, A. niger, T. mentagrophytes foram mais resistentes, embora uma importante acao letal da essencia tenha sido observada. Em 60 minutos, o oleo essencial de cravo (0,4% v.v.) em solucao concentrada de acucar reduziu em 99,6% um inoculo de 106 u.f.c./ml de T. mentagrophytes. O efeito letal do oleo essencial de cravo sobre C. albicans, apos 2 minutos de contato, foi semelhante ao de desinfectantes comumente usados em hospitais, como povidona-iodo e cloroxilenol.


Brazilian Journal of Microbiology | 2007

Disinfectant-resistant bacteria in Buenos Aires city hospital wastewater

Lidia Nuñez; J. Moretton

Large quantities of disinfectants are used in hospitals, externally on human skin or to eliminate microorganisms from inanimate objects. After use, residual quantities of these products reach the wastewater, exposing the bacteria that survive in hospital wastewaters to a wide range of biocides that could act as a selective pressure for the development of resistance. Increasing attention has been directed recently to the resistance of bacteria to disinfectants. The aim of this paper was to determine the disinfectant bacterial resistance pattern of the microflora released to the urban sewer system by hospital effluents. The characterization of the waste water microflora was performed by determination of the CFU of heterotrophic bacteria, fecal indicator bacteria, Pseudomonas sp. and Staphylococcus sp., in a Buenos Aires hospital effluent. The bacterial resistance to the disinfectants more frequently used in the hospital practice, glutaraldehyde, chlorhexidine and povidone-iodine, was then evaluated. Disinfectant resistant bacterial strains were isolated and typified. Between 103 and 106 chlorexidine resistant bacteria/100 mL were isolated from the samples. Bacteria resistant to other disinfectants ranged between 103 and 104 /100 mL. The bacterial population resistant to desinfectants to was mainly composed by Enterobacteriaceae, Staphylococcus spp, and Bacillus spp, which are highly associated to nosocomial infections. The results obtained show that the hospital effluents are of importance in the bacterial resistance selection process, particularly in the case of disinfectants.


Brazilian Journal of Microbiology | 2012

Microbicide activity of clove essential oil (Eugenia caryophyllata)

Lidia Nuñez; M. D' Aquino

Clove essential oil, used as an antiseptic in oral infections, inhibits Gram-negative and Gram-positive bacteria as well as yeast. The influence of clove essential oil concentration, temperature and organic matter, in the antimicrobial activity of clove essential oil, was studied in this paper, through the determination of bacterial death kinetics. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were the microorganisms selected for a biological test. To determine the temperature effect, they were assayed at 21° and 37° C. The concentration coefficient was determined with 0.4%, and 0.2% of essential oil. The influence of the presence of organic matter was determined with 0.4% of essential oil. The results obtained demonstrated that Escherichia coli were more sensitive even though the essential oil exerted a satisfactory action in three cases. In the three microbial species, 0.4% of essential oil at 21° C have reduced the bacterial population in 5 logarithmic orders. Organic matter reduces the antibacterial activity even though the bactericide efficacy was not lost. Clove essential oil can be considered as a potential antimicrobial agent for external use


Zentralblatt Fur Bakteriologie-international Journal of Medical Microbiology Virology Parasitology and Infectious Diseases | 1990

Botulinic Toxin Detection by Counterimmunoelectrophoresis

Lidia Nuñez; Ethel Amato de Lagarde; Miguel D'Aquino

A counterimmunoelectrophoretic (CIE) technique was developed to detect botulinic toxin type A, and the method was compared with the mouse bioassay. A 100 LD50 concentration was detected within 2 h. Crossed reactions were observed with antitoxins of types B and F. As regards other Clostridium species, precipitin lines were seen between C. sporogenes and antitoxin type A. Foodstuffs artificially contaminated with C. botulinum type A were assayed by means of CIE and mouse bioassay, without recording interference by substances normally contained in foodstuffs.


Rev. argent. infectol | 1997

Influencia de la materia orgánica sobre la acción de desinfectantes

Miguel D'Aquino; Lidia Nuñez


Revista Ambiente & Água | 2012

Bacterias resistentes a antibióticos en aguas grises como agentes de riesgo sanitario

Lidia Nuñez; Carina Tornello; Noel Puentes; Juan Moretton


Ambiente E Agua - An Interdisciplinary Journal of Applied Science | 2012

Health risks associated with the presence of antibiotic resistant bacteria in greywater

Lidia Nuñez; Carina Tornello; Noel Puentes; Juan Moretton


Ars Pharmaceutica | 2016

El efluente hospitalario como fuente de enterococos vancomicina resistentes

Lidia Nuñez; Carina Tornello; Noel Puentes; Elena Espigares; Elena Moreno; Miguel Espigares; Juan Moretton


Revista Internacional De Contaminacion Ambiental | 2014

Análisis de riesgo sanitario en aguas grises de la provincia de Buenos Aires, Argentina

Lidia Nuñez; Claudia Molinari; Marta Paz; Carina Tornello; Julián Mantovano; Juan Moretton


Acta toxicológica argentina | 2012

Evaluación ecotóxica y genotóxica de aguas residuales hospitalarias

Anahí Magdaleno; Ángela Beatriz Juárez; Marta Paz; Carina Tornello; Lidia Nuñez; Juan Moretton

Collaboration


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Carina Tornello

University of Buenos Aires

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Juan Moretton

University of Buenos Aires

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Miguel D'Aquino

University of Buenos Aires

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Noel Puentes

University of Buenos Aires

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Marta Paz

University of Buenos Aires

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Anahí Magdaleno

University of Buenos Aires

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Claudia Molinari

University of Buenos Aires

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J. Moretton

University of Buenos Aires

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Jorge Chirife

University of Buenos Aires

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Julián Mantovano

University of Buenos Aires

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