Lies Franssens

Intermittent thermal manipulations of broiler embryos during late incubation and their immediate effect on the embryonic development and hatching process.

Intermittent high (+3°C) and low (-3°C) temperature treatments for 4 h on embryonic day (E) 16, E17, and E18 showed differential effects on embryonic metabolism, without influencing embryonic growth or hatchability. Embryos in the high-temperature group shifted to a more anaerobic metabolism, as indicated by a lower partial pressure of O(2) and a higher partial pressure of CO(2) in the air cell, lower blood pH, and higher lactic acid production. Three hours after the end of the high-temperature treatment, a decrease in metabolism was observed, as indicated by the lower partial pressure of CO(2) and higher partial pressure of O(2) in the air cell and increased plasma triglyceride levels. The embryos in the low-temperature group responded by temporarily slowing down their metabolism, especially the metabolism of carbohydrates and lipids, as indicated by altered air cell gases, a higher relative yolk weight, higher plasma triglyceride level, and higher liver glycogen level. Three hours after the end of the temperature treatment, the metabolism of embryos in the low-temperature treatment had increased to the level of the control temperature group. However, for both temperature treatments, during the hatching process, all the shortages and excesses created were restored to control levels, which would explain the lack of change in embryo growth and hatchability and the slight delay in the hatching process. These mild consequences of the intermittent temperature treatment indicate that the different metabolic shifts made by the embryos seem to be efficient in overcoming the challenges of the intermittent high- or low-temperature treatment during late incubation.

Effect of the ratio of dietary n-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid on broiler breeder performance, egg quality, and yolk fatty acid composition at different breeder ages.

When added to the feed of broiler breeder hens, dietary polyunsaturated fatty acids (FA) can be incorporated into the yolk and therefore become available to the progeny during their early development. The mechanism involved in lipid metabolism and deposition in the egg may be influenced by breeder age. Before the effect of an elevated concentration of certain polyunsaturated FA on the embryo can be investigated, the effect at breeder level and egg quality must be further assessed. The aim of the present experiment was to evaluate the effects of dietary n-6/n-3 ratios and dietary eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) ratios, provided to broiler breeder hens, in terms of their zoo technical performance, egg quality, and yolk FA composition. Starting at 6 wk of age, 640 Ross-308 broiler breeder hens were fed 1 of 4 different diets. The control diet was a basal diet, rich in n-6 FA. The 3 other diets were enriched in n-3 FA, formulated to obtain a different EPA/DHA ratio of 1/1 (EPA = DHA), 1/2 (DHA), or 2/1 (EPA). In fact, after analysis the EPA/DHA ratio was 0.8, 0.4, or 2.1, respectively. Dietary EPA and DHA addition did not affect the performance of the breeder hens, except for egg weight. Egg weight was lower (P < 0.001) for all n-3 treatments. Dietary EPA improved number of eggs laid in the first 2 wk of the production cycle (P = 0.029). The absolute and relative yolk weight of eggs laid by EPA = DHA fed hens was lowest (P = 0.004 and P = 0.025, respectively). The EPA and DHA concentrations in the yolk were highly dependent on dietary EPA and DHA concentrations with a regression coefficient equal to 0.89. It can be concluded that dietary EPA and DHA can be incorporated in the breeder egg yolk to become available for the developing embryo, without compromising the performance and egg quality of the flock.

Spread of hatch and delayed feed access affect post hatch performance of female broiler chicks up to day 5.

It is not rare that newly hatched chicks remain without feed for about 24 to 48 h before they are placed on farms due to a series of logistic operations. Furthermore, the spread in hatching time can also mount up to 30 to 48 h for late v. early hatchers. In other words, the practice is a complex combination of spread of hatch and delayed feed access. The present study was aimed to investigate the combined effects of hatching time with a delay in feed access of 48 h, starting from their hatch-time (biological age). When chicks had access to feed immediately after hatch, late hatchers had a higher feed intake and relative growth rate up to day 5 compared with their early hatched counterparts. Feed deprivation during the first 48 h resulted in retarded early growth rate, which was further aggravated by an impaired feed intake after refeeding. In addition, the differential effects of hatching time on relative growth rate and feed intake observed in immediately fed chicks were eliminated by the 48 h feed delay. The yolk utilization after hatch was faster for the late hatchers up to biological day 2 regardless of the feeding treatments. Hatching muscle glycogen content was higher in the late hatchers compared with that of their early counterparts at hatch and at biological day 2 independent of feeding treatment. Moreover, the liver glycogen content of the late hatchers was also higher at hatch. For the immediately fed chicks, the proportional breast muscle weight of the late hatchers was higher at biological day 2 and 5. For the starved chicks, on the other hand, this effect was only observed after they had access to feed (biological day 5). The different plasma T3 levels at hatch may have contributed to the different post hatch performance. It is concluded that the spread of hatch influenced post hatch performance, especially appetite and growth at least until day 5. Moreover, the delay in feed access interacted with the hatching time and caused adverse effects on the post hatch performance.

Partial albumen removal early during embryonic development of layer-type chickens has negative consequences on laying performance in adult life

To examine the importance of albumen as a protein source during embryonic development on the posthatch performance of laying hens, 3 mL of the albumen was removed. At hatch, no difference in BW could be observed. Chicks from the albumen-deprived group had a lower residual yolk weight due to higher yolk utilization. During the rearing phase (hatch to 17 wk of age), the BW of the albumen-deprived pullets was lower compared with the control and sham pullets. The feed intake of the albumen-deprived pullets was also lower than the control pullets. However, during the laying phase (18 to 55 wk of age) these hens exceeded the control and sham hens in BW, although this was not accompanied by a higher feed intake. The albumen-deprived hens exhibited a lower egg production capacity as demonstrated by the reduced egg weight, laying rate, and egg mass and increased number of second grade eggs. In addition, the eggs laid by the albumen-deprived hens had a higher proportional yolk and lower proportional albumen weight. In conclusion, prenatal protein deprivation by albumen removal caused a long-lasting programming effect, possibly by differences in energy allocation, in favor of growth and maintenance and impairing reproductive performance.

Acid–base regulation during embryonic development in amniotes, with particular reference to birds

During avian embryonic development, the carbon dioxide tension inside the egg increases as the shell restricts gas exchange with the environment. Acid-base regulation of the avian embryo is a complex process, not only due to the non-function of the lungs and limited functionality of the kidneys but also because the embryo is affected by the inflow of bicarbonates from the shell, when calcium is reabsorbed for calcification. Moreover, interaction occurs between the embryo and the extraembryonic compartments. It has been shown that carbonic anhydrase plays a crucial role in the formation of sub-embryonic fluid and in the chorioallantoic membrane and kidney. This review provides a detailed overview of the acid-base status of the extraembryonic compartments inside chicken eggs, and their interaction in keeping the acid-base status of the embryo balanced. Secondly, experimental acid-base disturbances are summarized. The last part of the review briefly compares embryos of birds and other amniotes (mammals and reptiles) with regard to acid-base regulation.

Effects of maternal dietary EPA and DHA supplementation and breeder age on embryonic and post-hatch performance of broiler offspring

Breeder age and nutrition are amongst the most important factors affecting progeny growth and development. The present experiment was carried out to evaluate the effects of n-3 fatty acid (FA), with special emphasis on the ratio of eicosapentaenoic (EPA, 20:5 n-3) and docosahexaenoic (DHA, 22:6 n-3) acid, provided to the diet of ageing broiler breeder hens at different ratios, on the incubation parameters and the performance of the offspring. Four hundred and eighty Ross-308 broiler breeder hens were fed one of four different diets (120/treatment), with an equal fat content. The control diet was a basal diet, rich in n-6 FAs (CON). Blends of fish oil were used to enrich the three other diets in n-3 FA and to obtain different EPA/DHA ratios of 1/1 (EPA=DHA), 1/2 (DHA) or 2/1 (EPA). Every 5 weeks, incubation parameters were assessed. Every 15 weeks, offspring was reared until slaughter age on a standard diet. Breeder age affected almost all incubation and post-hatch parameters, whereas n-3 FA treatment only lowered egg weight (p < 0.0001) and consequently hatched chick weight (p < 0.0001). Supplementation of EPA resulted in a higher proportional liver weight (p = 0.0219) at hatch, a lower body weight up to 28 days post-hatch (p = 0.0418), a lower daily weight gain (p = 0.0498) and a higher feed conversion ratio (p = 0.0395) during the starter period (p = 0.0498), resulting in a higher overall offspring feed conversion ratio (p = 0.0317) compared to the control diet. DHA supplementation, on the other hand, resulted in a lower residual yolk weight (p = 0.0220) and a higher overall offspring mortality (p = 0.0125). In conclusion, supplementation of n-3 FA could not counter the adverse effect of breeder flock age, but did not harm incubation or improve post-hatch performance, either. EPA and DHA affected offspring development differently during early post-hatch life.

Transition of maternal dietary n-3 fatty acids from the yolk to the liver of broiler breeder progeny via the residual yolk sac

The aim of the present study was to evaluate the transfer of maternal dietary fatty acids (FA) from the yolk to the developing offspring, with special emphasis on n-3 FA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Six hundred forty Ross 308 breeders were housed from 6 to 58 wk of age in 16 pens resulting in 4 replicates per dietary treatment. They were fed 1 of 4 diets: a basal diet, rich in n-6 FA (CON), or an n-3 FA enriched diet formulated to obtain an EPA/DHA ratio of 1/1 (EPA=DHA), 1/2 (DHA), or 2/1 (EPA). At 28, 43, and 58 wk of age, 20 eggs per treatment were collected and analyzed for FA composition. At these same breeder ages, 600 fertilized eggs per treatment were incubated. At hatch the residual yolks of 25 chicks per treatment were collected and analyzed for FA composition. At every hatch, 180 chicks per treatment were raised under standard conditions and livers were sampled at d 1, 14, 28, and 38 d for FA analysis. Concentrations of EPA in the yolk and residual yolk of eggs laid by EPA-fed breeders were highest, next-to-highest for EPA=DHA-fed breeders, next-to-lowest for DHA-fed breeders, and lowest in those laid by control hens, reflecting the inclusion levels in the maternal diets. Yolk and residual yolk DHA concentrations, however, were not only elevated due to DHA supplementation, compared with the control diet, but also due to EPA supplementation. Offspring hepatic EPA concentrations were elevated until d 28 in all n-3 enriched groups, whereas hepatic DHA concentrations were only affected by EPA=DHA and DHA supplementation at d 1. No differences were found in hepatic DHA concentrations at later offspring ages. Considering the role of EPA and DHA in early development and growth, the maternal supply of these n-3 FA might improve offspring health and performance.

In ovo L‐arginine supplementation stimulates myoblast differentiation but negatively affects muscle development of broiler chicken after hatching

In this study, we tested the hypothesis that in ovo feeding (IOF) of L-arginine (L-Arg) enhances nitric oxide (NO) production, stimulates the process of myogenesis, and regulates post-hatching muscle growth. Different doses of L-Arg were injected into the amnion of chicken embryos at embryonic day (ED) 16. After hatching, the body weight of individual male chickens was recorded weekly for 3 weeks. During in vitro experiments, myoblasts of the pectoralis major (PM) were extracted at ED16 and were incubated in medium containing 0.01 mm L-Arg, 0.05 mm L-Arg, and (or) 0.05 mm L-nitro-arginine-methyl-ester (L-NAME), an inhibitor of nitric oxide synthase (NOS). When 25 mg/kg L-Arg/initial egg weight was injected, no difference was observed in body weight at hatch, but a significant decrease was found during the following 3 weeks compared to that of the non-injected and saline-injected control, and this also affected the growth of muscle mass. L-NAME inhibited gene expression of myogenic differentiation antigen (MyoD), myogenin, NOS, and follistatin, decreased the cell viability, and increased myostatin (MSTN) gene expression. 0.05 mm L-Arg stimulated myogenin gene expression but also depressed muscle cell viability. L-NAME blocked the effect of 0.05 mm L-Arg on myogenin mRNA levels when co-incubated with 0.05 mm L-Arg. L-Arg treatments had no significant influence on NOS mRNA gene expression, but had inhibiting effect on follistatin gene expression, while L-NAME treatments had effects on both. These results suggested that L-Arg stimulated myoblast differentiation, but the limited number of myoblasts would form less myotubes and then less myofibers, while the latter limited the growth of muscle mass.

Reduced protein availability by albumen removal during chicken embryogenesis decreases body weight and induces hormonal changes

What is the central question of this study? Prenatal protein undernutrition by albumen removal in an avian model of fetal programming leads to long‐term programming effects, but when do these effects first appear and are these programming effects regulated by the same candidate genes as in mammals? What is the main finding and its importance? The present results indicate that prenatal protein undernutrition by albumen removal induces phenotypical and hormonal changes in the early posthatch period, when the mismatch between the prenatal and postnatal environment first arises, but these changes are not accompanied by an altered gene expression of the selected candidate genes.

Prenatal tolbutamide treatment alters plasma glucose and insulin concentrations and negatively affects the postnatal performance of chickens

To examine the relationship of insulin and glucose, broiler embryos were subjected to acute or prolonged hypoglycemia during the late embryonic phase by, respectively, injecting once (at embryonic day [ED] 16 or 17) or on 3 consecutive days (ED 16, 17, and 18) with tolbutamide (80 μg/g embryo weight), a substance that stimulates insulin secretion from the pancreas. After 1 tolbutamide injection, a prolonged (32 h) decrease of plasma glucose and a profound acute increase in plasma insulin were observed. The 3 consecutive tolbutamide injections induced hypoglycemia for 4 days (from ED 16 to ED 19). The postnatal performance after 3 consecutive tolbutamide injections in broiler embryos was also investigated. Body weight was lower in tolbutamide-treated chickens from hatch to 42 d compared with sham (P = 0.001) and control (P < 0.001) chickens. Feed intake was lower in the tolbutamide group from hatch to 42 d as compared with sham (P = 0.007) and control (P = 0.017) animals. In addition, at 42 d, plasma glucose concentrations, after an insulin injection challenge (50 μg/kg body weight), were higher in tolbutamide-treated chickens compared with the sham and the control group as were their basal glucose levels (P value of group effect <0.001). In conclusion, tolbutamide treatment during the late embryonic development in broilers resulted in prolonged hypoglycemia in this period and negatively influenced the posthatch performance.