Liliane Millet
Institut national de la recherche agronomique
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Publication
Featured researches published by Liliane Millet.
Journal of Dairy Research | 2004
Cécile Callon; Liliane Millet; Marie-Christine Montel
The objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk Salers cheeses at the species and strain levels. The characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: Rep-PCR, species and genus specific amplifications and the sequence analysis of 16S rDNA for strain typing and taxonomic identification. The strains belonged to Lactobacillus plantarum, Lactobacillus paracasei, Lactococcus lactis, Lactococcus garviae, Enterococcus faecalis, Enterococcus faecium, Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Streptococcus salivarius, Streptococcus millieri, Streptococcus macedonicus and Pediococcus pentosaceus. A wide phenotypic and genomic heterogeneity was observed within the different species (Lactobacillus plantarum, Lactobacillus paracasei and Leuconostoc mesenteroides) according to the origin and the time of ripening. The natural microflora was different from strain collection and each method must be combined to identify and characterize natural microflora. This study revealed the low selectivity of selective media used for the isolation of different groups of lactic acid bacteria except the Facultatively Heterofermentative lactobacilli medium selecting mesophile lactobacilli and SB medium selective for Enterococcus. The study reveals, for the first time, the microbial lactic acid bacteria community of Salers cheese and its diversity. A better knowledge of microbial flora will be useful to improve understanding of sensory quality of cheeses.
Systematic and Applied Microbiology | 1999
Laure Gory; Liliane Millet; Jean-Jacques Godon; Marie-Christine Montel
16S rRNA targeted oligonucleotide probes were designed by sequence analysis of an rRNA database to discriminate S. carnosus, S. warneri, and S. saprophyticus species. After establishing hybridization conditions by RNA dot blot hybridization with reference species, our probes were shown to be specific. By in situ hybridization only S-S-S.carno-0440-a-A-23 and S-S-S.war-0180-a-A-23 can specifically detect S. carnosus and S. warneri, respectively. The detection of old cells of S. carnosus 833 was more limited by the permeabilisation than by the low rRNA content. One day old cells could be permeabilized with lysostaphin, whereas young cells were permeabilized with lysozyme.
Applied and Environmental Microbiology | 1999
V. Julliand; Albane de Vaux; Liliane Millet; Gérard Fonty
Reproduction Nutrition Development | 2001
Brigitte Michalet-Doreau; Isabelle Fernandez; Céline Peyron; Liliane Millet; G. Fonty
International Journal of Food Microbiology | 2007
M. Saubusse; Liliane Millet; Céline Delbès; Cécile Callon; Marie-Christine Montel
Microbial Ecology in Health and Disease | 2003
Nadia Bennegadi; G. Fonty; Liliane Millet; T. Gidenne; Dominique Licois
Reproduction Nutrition Development | 2001
C. Martin; Liliane Millet; G. Fonty; Brigitte Michalet-Doreau
Fems Microbiology Letters | 1991
André Breton; Martine Dusser; Brigitte Gaillard-Martinie; Jean Guillot; Liliane Millet; Gérard Prensier
Fems Microbiology Letters | 1993
Valérie Michel; Gérard Fonty; Liliane Millet; Frédérique Bonnemoy; Philippe Gouet
Lait | 1974
Liliane Millet; Dominique Melcion; J. J. Devoyod