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Featured researches published by Liliane Millet.


Journal of Dairy Research | 2004

Diversity of lactic acid bacteria isolated from AOC Salers cheese.

Cécile Callon; Liliane Millet; Marie-Christine Montel

The objective of this work was to describe the diversity of lactic acid bacteria in traditional raw milk Salers cheeses at the species and strain levels. The characterization of 381 strains isolated during ripening and various strain collections was investigated using physiological analysis and molecular techniques: Rep-PCR, species and genus specific amplifications and the sequence analysis of 16S rDNA for strain typing and taxonomic identification. The strains belonged to Lactobacillus plantarum, Lactobacillus paracasei, Lactococcus lactis, Lactococcus garviae, Enterococcus faecalis, Enterococcus faecium, Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Streptococcus salivarius, Streptococcus millieri, Streptococcus macedonicus and Pediococcus pentosaceus. A wide phenotypic and genomic heterogeneity was observed within the different species (Lactobacillus plantarum, Lactobacillus paracasei and Leuconostoc mesenteroides) according to the origin and the time of ripening. The natural microflora was different from strain collection and each method must be combined to identify and characterize natural microflora. This study revealed the low selectivity of selective media used for the isolation of different groups of lactic acid bacteria except the Facultatively Heterofermentative lactobacilli medium selecting mesophile lactobacilli and SB medium selective for Enterococcus. The study reveals, for the first time, the microbial lactic acid bacteria community of Salers cheese and its diversity. A better knowledge of microbial flora will be useful to improve understanding of sensory quality of cheeses.


Systematic and Applied Microbiology | 1999

Identification of Staphylococcus carnosus and Staphylococcus warneri Isolated from Meat by Fluorescent in situ Hybridization with 16S rRNA-Targeted Oligonucleotide Probes

Laure Gory; Liliane Millet; Jean-Jacques Godon; Marie-Christine Montel

16S rRNA targeted oligonucleotide probes were designed by sequence analysis of an rRNA database to discriminate S. carnosus, S. warneri, and S. saprophyticus species. After establishing hybridization conditions by RNA dot blot hybridization with reference species, our probes were shown to be specific. By in situ hybridization only S-S-S.carno-0440-a-A-23 and S-S-S.war-0180-a-A-23 can specifically detect S. carnosus and S. warneri, respectively. The detection of old cells of S. carnosus 833 was more limited by the permeabilisation than by the low rRNA content. One day old cells could be permeabilized with lysostaphin, whereas young cells were permeabilized with lysozyme.


Applied and Environmental Microbiology | 1999

Identification of Ruminococcus flavefaciens as the predominant cellulolytic bacterial species of the equine cecum

V. Julliand; Albane de Vaux; Liliane Millet; Gérard Fonty


Reproduction Nutrition Development | 2001

Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents

Brigitte Michalet-Doreau; Isabelle Fernandez; Céline Peyron; Liliane Millet; G. Fonty


International Journal of Food Microbiology | 2007

Application of Single Strand Conformation Polymorphism PCR method for distinguishing cheese bacterial communities that inhibit Listeria monocytogenes

M. Saubusse; Liliane Millet; Céline Delbès; Cécile Callon; Marie-Christine Montel


Microbial Ecology in Health and Disease | 2003

Effects of age and dietary fibre level on caecal microbial communities of conventional and specific pathogen-free rabbits

Nadia Bennegadi; G. Fonty; Liliane Millet; T. Gidenne; Dominique Licois


Reproduction Nutrition Development | 2001

Cereal supplementation modified the fibrolytic activity but not the structure of the cellulolytic bacterial community associated with rumen solid digesta

C. Martin; Liliane Millet; G. Fonty; Brigitte Michalet-Doreau


Fems Microbiology Letters | 1991

Piromyces rhizinflata nov. sp., a strictly anaerobic fungus from faeces of the Saharian ass: a morphological, metabolic and ultrastructural study

André Breton; Martine Dusser; Brigitte Gaillard-Martinie; Jean Guillot; Liliane Millet; Gérard Prensier


Fems Microbiology Letters | 1993

In vitro study of the proteolytic activity of rumen anaerobic fungi

Valérie Michel; Gérard Fonty; Liliane Millet; Frédérique Bonnemoy; Philippe Gouet


Lait | 1974

La flore microbienne du fromage de Cantal fabriqué à partir de lait cru. III. - Rôle des levures dans la maturation de la "tome"

Liliane Millet; Dominique Melcion; J. J. Devoyod

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Marie-Christine Montel

Institut national de la recherche agronomique

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A. Breton

Blaise Pascal University

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Cécile Callon

Institut national de la recherche agronomique

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G. Fonty

Institut national de la recherche agronomique

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Brigitte Michalet-Doreau

Institut national de la recherche agronomique

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Céline Delbès

Institut national de la recherche agronomique

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Frédérique Duthoit

Institut national de la recherche agronomique

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Jean Guillot

Institut national de la recherche agronomique

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Jean-Jacques Godon

Institut national de la recherche agronomique

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Philippe Gouet

Institut national de la recherche agronomique

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