Lina Tang

Preparation of Graphene-Modified Acupuncture Needle and Its Application in Detecting Neurotransmitters

We report a unique nanosensing platform by combining modern nanotechnology with traditional acupuncture needle to prepare graphene-modified acupuncture needle (G-AN), and using it for sensitive detection of neurotransmitters via electrochemistry. An electrochemical deposition method was employed to deposit Au nanoparticles (AuNPs) on the tip surface of the traditional acupuncture needle, while the other part of the needle was coated with insulation paste. Subsequently, the G-AN was obtained by cyclic voltammetry reduction of a graphene oxide solution on the surface of the AuNPs. To investigate the sensing property of the G-AN, pH dependence was measured by recording the open circuit potential in the various pH buffer solutions ranging from 2.0 to 10.0. What’s more, the G-AN was further used for detection of dopamine (DA) with a limit of detection of 0.24 μM. This novel G-AN exhibited a good sensitivity and selectivity, and could realize direct detection of DA in human serum.

In vivo Monitoring of Serotonin by Nanomaterial Functionalized Acupuncture Needle

Acupuncture treatment is amazing but controversial. Up to now, the mechanism of treating diseases by acupuncture and moxibustion is still unclear, especially the occurrence of the molecular events in local acupoints. Herein, we report an extremely stable microsensor by modifying carbon nanotube (CNT) to the tip surface of acupuncture needle and applying this CNT-modified acupuncture needle for real time monitoring of serotonin (5-HT) in vivo. To stabilize CNT modification on the needle tip surface, poly(3,4-ethylenedioxythiophene)(PEDOT) was employed as glue water to stick CNT on the needle. The detection limit of the CNT-modified needle was found to be approximately 50 nM and 78 nM in the PBS and the cell medium, respectively. In addition, the needle showed good selectivity to some inflammatory mediators and some electroactive molecules. For the first time, the CNT-modified needle could be directly probed into rat body for real time monitoring of 5-HT in vivo, showing a great potential for better understanding the mechanism of acupuncture treatment.

Determination of Rutin by a Graphene-Modified Glassy Carbon Electrode

A reduced graphene oxide-modified glassy carbon electrode for sensitive detection of rutin is reported. The modified electrode was obtained by one-step electrochemical reduction of graphene oxide on the bare glassy carbon electrode. In the presence of graphene, an enhanced electrochemical response for rutin appeared with a pair of well-defined anodic and cathodic peaks in pH 3.0 phosphate buffer. Under the optimized conditions, the anodic peak currents exhibited a linear relationship with rutin concentration from 0.1 to 2.0 µM with a detection limit of 23.2 nM. The modified electrode was employed to the analysis of tablets (with satisfactory recovery of 19.96 mg/per tablet) and Flos Sophorae. The graphene-modified electrode exhibited high sensitivity, good stability, and selectivity for the determination of rutin.

A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats

This study reports an acupuncture needle modified with an iron-porphyrin functionalized graphene composite (FGPC) for real-time monitoring of nitric oxide (NO) release in acupoints of rats. A gold film was first deposited to the needle surface to enhance the conductivity. The FGPC was prepared via hydrothermal synthesis, and subsequently applied to the tip surface of acupuncture needle by electrochemical deposition method. The functionalized needle enabled a specific and sensitive detection of NO based on the favorably catalytic properties of iron-porphyrin and the excellent conductivity of graphene. Amperometric data showed that the needle achieved not only a low detection limit down to 3.2 nM in PBS solution, but also a satisfactory selectivity. Interestingly, the functionalized needle could be inserted into the acupoints of rats for real-time monitoring of NO in vivo. It was found that a remarkable response to NO was respectively obtained in different acupoints when stimulated by L-arginine (L-Arg), revealing that the release of NO was detectable in acupoints. We expect this work would showcase the applications of acupuncture needle in detecting some important signaling molecules in vivo, and exploring the mechanism of acupuncture treatment.

Electrochemical Determination of Dopamine by a Reduced Graphene Oxide–Gold Nanoparticle-Modified Glassy Carbon Electrode

ABSTRACT A novel electrochemical aptasensor is reported for the label-free determination of dopamine by electrochemically reduced graphene oxide and a gold nanocomposite with a ribonucleic acid (RNA) aptamer. The nanocomposite was fabricated by layer-to-layer electrochemical deposition. The aptamer was immobilized on the surface of the gold nanoparticles through the formation of thiol–gold bonds. Dopamine was determined through specific interaction with the immobilized aptamer. The redox activity of the analyte enabled direct electrochemical analysis. The conductivity and structure of the nanocomposite were characterized by cyclic voltammetry and scanning electron microscopy. In contrast to bare or reduced graphene oxide-modified electrodes, the nanocomposite-modified electrode significantly enhanced the current response. The electrochemical aptasensor provided a detection limit of 0.13 µM with a linear dynamic range from 0.5 to 20 µM. In addition, the sensor was shown to provide high selectivity and satisfactory stability for the determination of dopamine in the presence of analogs and in human serum.

Identification of Chinese Herbs Using a Sequencing-Free Nanostructured Electrochemical DNA Biosensor

Due to the nearly identical phenotypes and chemical constituents, it is often very challenging to accurately differentiate diverse species of a Chinese herbal genus. Although technologies including DNA barcoding have been introduced to help address this problem, they are generally time-consuming and require expensive sequencing. Herein, we present a simple sequencing-free electrochemical biosensor, which enables easy differentiation between two closely related Fritillaria species. To improve its differentiation capability using trace amounts of DNA sample available from herbal extracts, a stepwise electrochemical deposition of reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) was adopted to engineer a synergistic nanostructured sensing interface. By using such a nanofeatured electrochemical DNA (E-DNA) biosensor, two Chinese herbal species of Fritillaria (F. thunbergii and F. cirrhosa) were successfully discriminated at the DNA level, because a fragment of 16-mer sequence at the spacer region of the 5S-rRNA only exists in F. thunbergii. This E-DNA sensor was capable of identifying the target sequence in the range from 100 fM to 10 nM, and a detection limit as low as 11.7 fM (S/N = 3) was obtained. Importantly, this sensor was applied to detect the unique fragment of the PCR products amplified from F. thunbergii and F. cirrhosa, respectively. We anticipate that such a direct, sequencing-free sensing mode will ultimately pave the way towards a new generation of herb-identification strategies.

Photocatalytic self-cleaning electrochemical sensor for honokiol based on a glassy carbon electrode modified with reduced graphene oxide and titanium dioxide

AbstractThe authors describe the construction of a renewable electrochemical method for determination of honokiol in complex traditional Chinese herbs. A nanocomposite consisting of reduced graphene oxide and titanium dioxide was prepared and deposited on the surface of a glassy carbon electrode (GCE). This composite endows the GCE with self-cleaning capability via ultraviolet illumination. Under optimized conditions and at a typical working voltage of 0.54 V (vs. Ag/AgCl), the sensor has a linear response in the 0.025 to 10 μmol·L−1 honokinol concentration range, and the detection limit is 20 nmol·L−1 (at an S/N ratio of 3). The method was successfully applied to the determination of honokiol in cortex herbs and its galenicals. Graphical abstractSchematic of a photocatalytic self-cleaning electrochemical sensor based on reduced graphene oxide and titanium dioxide for honokiol detection. The method has successfully been applied to the determination of honokiol in Chinese herbs with satisfied results.