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Dive into the research topics where Lingyan Zheng is active.

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Featured researches published by Lingyan Zheng.


Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology | 2010

Expression of Toll-like receptors 7, 8, and 9 in primary Sjögren's syndrome

Lingyan Zheng; Zhiyuan Zhang; Chuangqi Yu; Chi Yang

OBJECTIVE The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögrens syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients. METHODS TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test. RESULTS TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively (P < .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands (P < .05). CONCLUSION TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.


Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology | 2009

Association between IFN-α and primary Sjogren's syndrome

Lingyan Zheng; Zhiyuan Zhang; Chuangqi Yu; Liwei Tu; Lai-ping Zhong; Chi Yang

OBJECTIVE To determine the level of IFN-alpha in labial salivary glands, plasma, and peripheral blood cells from patients with primary Sjogrens syndrome (pSS). METHODS Labial salivary gland biopsy specimens, plasma, and peripheral blood cells from patients with pSS were investigated. The IFN-alpha-positive cells, measurable IFN-alpha level, and IFN-alpha gene mRNA level were determined by using immunohistochemistry, ELISA, and real-time PCR, respectively. Statistical analysis was performed by Student t test or Fishers exact test. RESULTS About 60% of patients (22/37) with pSS had significantly higher scores of IFN-alpha-positive cells in labial gland biopsy and most IFN-alpha-positive cells were localized predominantly in the lymphocytes and ductal epithelial cells. But in 3 of the control samples (3/24), the IFN-alpha-positive cells existed only in the ductal epithelial cells with lower scores. Forty-three percents of the patients with pSS were found with detectable IFN-alpha concentration in plasma (> or = 12.5 pg/mL), and their concentration was higher than that of control group. Furthermore, the IFN-alpha mRNA levels in peripheral blood cells were up-regulated in the patients with pSS. CONCLUSION No matter in labial salivary glands or hematoplasma, or peripheral blood cells, IFN-alpha expression levels are up-regulated in patients with primary Sjogrens syndrome.


International Journal of Oral and Maxillofacial Surgery | 2010

Cervical necrotizing fasciitis due to methicillin-resistant Staphylococcus aureus: a case report

Wu-Chang Zhang; X.-Y. Cai; C. Yang; Longnv Zhou; M. Cai; Xiaofeng Lu; Lingyan Zheng; Boren Jiang

Cervical necrotizing fasciitis is an uncommon but potentially fatal infection characterized by rapidly progressive, widespread necrosis of the superficial fascia. The authors report a case of cervical necrotizing fasciitis of odontogenic origin in a male with uncontrolled diabetes mellitus. An early diagnosis was based on clinical examination, confirmed by computed tomography (CT) scan, which showed multiple collections of air in the left submandibular, submental and cervical region. Broad spectrum antibiotic therapy was started quickly followed by surgical drainage and debridement. Pus culture was positive for methicillin-resistant Staphylococcus aureus. Four days after admission, mediastinitis was revealed by CT and drainage was conducted through a transcervical incision. The patient was treated successfully with antimicrobial therapy, repeated surgical debridement and supportive care.


Journal of Oral and Maxillofacial Surgery | 2010

Endoscopic observation and strategic management of obstructive submandibular sialadenitis.

Chuangqi Yu; Chi Yang; Lingyan Zheng; Daming Wu

PURPOSE Obstructive submandibular sialadenitis is a relatively common disease in the clinic. The present study explored the cause and strategic management of chronic obstructive sialadenitis using sialoendoscopy and surgery. PATIENTS AND METHODS From January 2005 to October 2007, 128 patients with obstructive symptoms in the submandibular gland were diagnosed using sialoendoscopy and occlusal films, and the obstructions were removed using interventional sialoendoscopy, surgery only, or combined techniques, depending on the size, shape, site, and quality of the sialolith in the duct. The shape and structure of the hilus of the submandibular gland was observed using sialoendoscopy. RESULTS In the submandibular gland, the histologic features of 128 cases were identified endoscopically and radiographically. The endoscopic findings were of 3 types: sialolith in 114 (89%), mucus plug in 8 (6%), and stenosis in 6 (5%). Of 51 obstructions treated surgically, 47 were removed successfully, for a success rate of 92%. Of 63 obstructions treated using interventional sialoendoscopy, 52 were removed directly by sialoendoscopy, for a success rate of 83%. The unsuccessful cases were treated using sialoendoscopy or surgery. The obstructive symptoms were relieved in 12 of 14 patients without stones using dilation and irrigation under sialoendoscopy. Of the 114 patients with a stone, the sialoliths of 67 (59%) were located in the distal region (behind the first molar). The results showed that the more posterior the stone, the more difficult it was to remove. Three patients with treatment failure ultimately underwent resection of the gland. A basin-like structure in the hilus region was found in 86 (67%) of the 128 patients by sialoendoscopy. Finally, 5 patients were found to have a foreign body (fish bone) surrounded by a sialolith. CONCLUSIONS Sialoendoscopy is a useful new technique, not to only detect the cause of obstruction in a submandibular gland, but also to treat the obstructive submandibular sialadenitis more effectively if incorporated with conventional surgical approaches.


Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology | 2008

Causes of chronic obstructive parotitis and management by sialoendoscopy

Chuangqi Yu; Lingyan Zheng; Chi Yang; Ning Shen

BACKGROUND Sialoendoscopy is a relatively new technique to detect causes of obstruction in the parotid gland directly and manage the chronic obstructive parotitis effectively combined with continuous lavage and drug perfusion simultaneously. OBJECTIVES To describe the cause, exploration, and combined management of chronic obstructive parotitis using sialoendoscopy. STUDY DESIGN Between October 2004 and June 2006, 23 patients with obstructive symptoms were diagnosed by sialography and explored by diagnostic sialoendoscopy. The obstructions were then removed by interventional sialoendoscopy. After obstructions were removed successfully, 0.25% chloramphenicol was used to lavage the duct continuously, and then 40% iodized oil was perfused into duct. The results of follow-up were evaluated by visual analog scales (VAS) of the clinical appearances at different stages. RESULTS Twenty of the 23 patients were found with various types of stenosis and dilatation of duct on sialography, and 21 patients were explored using sialoendoscopy successfully. The features of these 21 cases found endoscopically were of 4 types: sialolith (n = 4; 19.0%), duct polyps (n = 5; 23.8%), stenosis (n = 3; 14.3%), and mucus plug (n = 9; 42.9%). Seventeen cases were treated successfully, removing obstructions via sialoendoscopy, giving a success rate of 80.9% (17 out of 21). The satisfactory rate after 6 months was 82.4% by VAS and secretion observation. CONCLUSION Sialoendoscopy is a promising image-guided technique for evaluation and treatment of obstructive disease of the parotid salivary glands.


Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology | 2009

Expression of interferon regulatory factor 1, 3, and 7 in primary Sjögren syndrome

Lingyan Zheng; Chuangqi Yu; Zhiyuan Zhang; Chi Yang; Xieyi Cai

OBJECTIVE The aim was to investigate the level of interferon regulatory factor (IRF) 1, 3, and 7 in peripheral blood cells from patients with primary Sjogren syndrome (pSS) and to determine whether and where IRF1 exists in the parotid glands of pSS. METHODS Peripheral blood cells and parotid gland biopsy specimens from patients with pSS were studied. The IRF1, IRF3, and IRF7 gene mRNA levels in peripheral blood cells were calculated by using real-time PCR. The IRF1-positive cells in the parotid glands with pSS were observed by using immunohistochemistry and immunofluorescence. Statistical analysis was performed by Student t test. RESULTS Compared with 24 control samples, the IRF1 mRNA levels in peripheral blood cells of 37 cases with pSS were up-regulated (P < .05), but the IRF3 and IRF7 mRNA levels of pSS were not up-regulated (P > .05). Relative quantitative levels of IRF1 mRNA were 2.17-fold higher in pSS patients than control subjects. The IRF1-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands. In all control subjects, the IRF1-positive cells were localized only to the ductal epithelial cells of parotid glands as determined by immunohistochemical staining or immunofluorescence. The scores of IRF1-positive cells of pSS were significantly higher than that of control samples (P < .05). CONCLUSION These findings indicate that IRF1 mRNA levels are up-regulated in the peripheral blood cells of pSS patients. Also, IRF1-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of healthy control subjects.


Arthritis Research & Therapy | 2016

Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome

Huan Shi; Ningning Cao; Yiping Pu; Lisong Xie; Lingyan Zheng; Chuangqi Yu

BackgroundTo examine the roles of long noncoding RNAs (lncRNAs) in the regulation of primary Sjögren’s syndrome (pSS) and reveal the expression profile of lncRNAs in labial salivary glands (LSGs) in pSS patients.MethodThe expression of 63,431 lncRNAs and 39,887 mRNAs were determined in the LSG of four pSS patients and four healthy controls using microarray experiments. Validation was performed in 30 pSS patients and 16 controls using real-time PCR. LncRNA-mRNA co-expression and gene-pathway networks were constructed using bioinformatics software.ResultA total of 1243 lncRNAs (upregulated: 890, downregulated: 353) and 1457 mRNAs (upregulated: 1141, downregulated: 316) were differentially expressed in the LSGs of pSS patients (fold change >2, P <0.05). Eight of these lncRNAs were validated using real-time PCR. ENST00000420219.1 (3.13-fold), ENST00000455309.1 (2.51-fold), n336161 (2.45-fold), NR_002712 (2.41-fold), ENST00000546086.1 (1.94-fold), Lnc-UTS2D-1:1 (1.79-fold), n340599 (1.69-fold), and TCONS_l2_00014794 (1.28-fold) were significantly upregulated in pSS. There were strong correlations between these lncRNAs and β2 microglobulin, disease course, erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), IgA, IgM, visual analogue scale (VAS) of parotid swelling and VAS of dry eyes. Computational analyses revealed that 28 of the differentially expressed (DE) mRNAs were associated with eight DE lncRNAs involved in chemokine signaling pathways, the nuclear factor-kappa B (NF-κB) signaling pathway, and tumor necrosis factor (TNF) signaling pathway.ConclusionsOur study revealed the expression profile of lncRNAs in LSGs of pSS patients. Many novel lncRNA transcripts that play important roles in the pathogenesis of pSS were dysregulated in pSS. Therefore, this study will aid in the development of new diagnostic biomarkers and drug therapies.


Journal of Oral and Maxillofacial Surgery | 2012

Is There Association Between Severe Multispace Infections of the Oral Maxillofacial Region and Diabetes Mellitus

Lingyan Zheng; Chi Yang; Weijie Zhang; Xieyi Cai; Eric S. Kim; Bin Jiang; Baoli Wang; Yiping Pu; Jin Wang; Zhiyuan Zhang; Longnv Zhou; Jian Zhou; Xin Guan

PURPOSE This study aims to identify the impact of diabetes on the final outcome (length of hospital stay and development of complications) in patients with severe multispace infections in the head and neck. Furthermore, it intends to characterize significant clinical features of these patients compared with nondiabetics. MATERIALS AND METHODS A retrospective study was conducted in 117 patients who received treatment for multispace infections of the head and neck region from 2007 through 2010 at the Department of Oral and Maxillofacial Surgery, Shanghai Ninth Hospital. The study identified diabetic patients and compared them with nondiabetic patients. Demographics, etiology of infection, clinical parameters (time from first onset of symptoms to hospital admission, number of spaces affected, and distribution of involved spaces), and laboratory values (bacteriology, admission blood glucose level, admission white blood cell count, and percentage of neutrophils on admission) were analyzed for clinical significance. Statistical analyses of the results between groups were performed using the Student t test, χ(2) test, variance analysis, logistic regression analysis, and linear regression analysis. RESULTS Admission blood glucose level was the only factor influencing the complications in multispace infections in the oral-maxillofacial region. Compared with nondiabetic patients, diabetics had infections that involved more spaces, longer hospital stays, and more frequent complications. Some diabetic patients died. The disease status (with or without diabetes) was associated with clinical outcomes (length of hospital stay and complications) in the therapy procedures. CONCLUSIONS This study identifies uncontrolled diabetes mellitus as an important indicator of clinical features and outcomes in treating multispace infections of the oral-maxillofacial region.


Journal of Oral and Maxillofacial Surgery | 2014

Four Osteotomy Methods With Piezosurgery to Remove Complicated Mandibular Third Molars: A Retrospective Study

Jing Ge; Chi Yang; Zheng Jw; Dongmei He; Lingyan Zheng; Yingkai Hu

PURPOSE Piezosurgery has been used widely in oral and maxillofacial surgery, but there has been no report systematically describing an osteotomy method with piezosurgery for complicated mandibular third molar removal. The aim of this study was to introduce 4 osteotomy methods using piezosurgery and evaluate their effects. MATERIALS AND METHODS A retrospective study was conducted of patients with a complicated impacted mandibular third molar requiring extraction. The predictor variable was the extraction technique. Four osteotomy methods using piezosurgery were tested according to different impaction types: method 1 involved complete bone removal; method 2 involved segmental bone removal; method 3 involved bone removal combined with tooth splitting; and method 4 involved block bone removal. Outcome variables were success rate, operative time, major complications (including nerve injury, mandible fracture, severe hematoma, or severe edema), and serious pyogenic infection. Data were analyzed using descriptive statistics. RESULTS The study was composed of 55 patients with 74 complicated impacted mandibular third molars. All impacted mandibular third molars were removed successfully. The average surgical time was 15 minutes (range, 8 to 26 minutes). Thirty-eight molars (51.4%) were extracted by method 1, 18 molars (24.3%) by method 2, 12 molars (16.2%) by method 3, and 6 molars (8.1%) by method 4. Two cases (2.7%) developed postoperative infections and recovered within 1 week using drainage and antibiotic administration. CONCLUSION The 4 osteotomy methods with piezosurgery provide effective ways of removing complicated impacted mandibular third molars.


Journal of Craniofacial Surgery | 2014

Foreign body induced sialolithiasis treated by sialoendoscopic intervention.

Lisong Xie; Lingyan Zheng; Chuangqi Yu; Chi Yang; Zuozhi Chen; Bai Yun; Eric S. Kim

Objective The study aims to identify the impact of sialolith formation by reviewing the foreign body induced sialolithiasis treated by sialoendoscopic intervention. Methods The study group included 13 patients whose sialolithiasis was induced by foreign body. After the routine radiographic examination, sialoendoscopic procedures were performed. Then, the treatment protocol was designed. Results The occupations of the 13 patients included 5 fishermen, 3 office workers, 2 workers, 1 teacher, 1 farmer, and 1 retired police officer. All patients had a unique diet habit—seafood. Eleven patients had a remembered incident of implanted fish bone and the following symptoms, with either obstructions or infections. Only 2 of the 13 had no memory of such an injury. All the stones were in the ducts of submandibular glands. In 10 procedures, there was 1 solitary stone, whereas 2 stones were encountered in 3 procedures. After being removed, 16 stones were crushed to expose the fish bone nidus of the stone. There was relief of symptoms after the procedures. Conclusions This study supported the possibility that some sialoliths resulted from a retrograde migration within the salivary ducts. In our study, the occupations (fisherman), the diet habit (seafood), and the injury history (a remembered incident of implanted fish bone and the following symptoms) were obviously related to the stone formation that was induced by the fish bone.

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Chuangqi Yu

Shanghai Jiao Tong University

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Chi Yang

Shanghai Jiao Tong University

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Huan Shi

Shanghai Jiao Tong University

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Lisong Xie

Shanghai Jiao Tong University

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Yiping Pu

Shanghai Jiao Tong University

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Zhiyuan Zhang

Shanghai Jiao Tong University

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Xieyi Cai

Shanghai Jiao Tong University

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ZhiJun Wang

Shanghai Jiao Tong University

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Baoli Wang

Shanghai Jiao Tong University

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Bin Jiang

Shanghai Jiao Tong University

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