Liu Rongchang

Development of a TaqMan-based real-time PCR assay for the rapid and specific detection of pigeon torque teno virus

Pigeon torque teno virus (PTTV), a recently discovered circular DNA virus. Here, we developed a TaqMan-based real-time PCR for rapid and specific detection of PTTV infections with sensitivity up to 49.3 copies/μl. Positive signals can be observed by the assay in pigeon embryonated eggs, which indicted that PTTV can be transmitted vertically. Our findings play important implications for a better understanding the transmission of torque teno virus in pigeons.

鸭瘟病毒的分离鉴定及其 UL 2、 TK 基因序列分析

2015年，福建省福州地区2个鸭场（M18肉鸭场及蛋鸭场）发生大量鸭死亡，初诊疑似鸭瘟。为明确其病原，分别采集病死鸭肝脏、食道样品进行鸭瘟病毒特异性PCR检测和病毒分离，对获得的2株病毒分离株分别进行鸭已知病原的检测，确定均为鸭瘟病毒。对新分离鉴定的2株鸭瘟病毒株的UL2基因进行序列测定及分析发现，均具有强毒株的分子特征；经TK基因序列测定及分析表明，2株新分离的鸭瘟病毒株与强毒代表株、弱毒疫苗株的TK基因核苷酸同源性均高达98.7%。