Liu Yingbin

Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues

The combined use of synchrotron radiation X-ray fluorescence (SRXRF) and isoelectric focusing (IEF) has been developed that allows comparison of the distribution of metal-containing proteins in cytosol of human hepatocellular carcinoma (HCC) with its surrounding “normal” tissues. Proteins in samples from two individuals were separated with thin layer IEF, and the contents of Fe, Cu and Zn in protein bands were determined with SRXRF after drying the IEF gel. The results show that the metal-containing proteins in these samples of hepatocellular cytosol are specific for a given patient, and the distribution patterns of metal-containing proteins are distinguishable between the HCC and “normal” tissues. The technique has the advantages of quantifying trace elements and identifying proteins simultaneously.

Peng’s pancreaticojejunal anastomosis (binding pancreaticojejunostomy) for the pancreatoduodenectomy —A new procedure ensuring no leakage

ObjectiveTo evaluate the safety and feasibility of a new operative procedure called binding pancreaticojejunostomy (BPJ) for the prevention of pancreatic leakage after pancreatoduodenectomy (PD).MethodsBinding pancreaticojejunostomy was performed in 100 patients from 1996 to 2000. During the operation, the cut end of the jejunum (3 cm) was everted, the everted mucosa of the jejunum was destroyed with carbolic acid. Meanwhile 3 cm long remnant of pancreas was isolated and sutured to 3 cm away form the jejunum cut end, care being taken not to penetrate the sero—muscular layer. Then, the everted jejunum was restituted to its normal position and the remnant of the pancreas was naturally pushed into the jejunal lumen for 3 cm. Finally, the surface of pancreatic remnant was closely in contact with destroyed jejunal mucosa surface, and a piece of absorbable thread was used to bind circumferentially this jejunum and the pancreatic remnant together, so no gap existed between the jejunal mucosa and pancreatic remnant.ResultsNo pancreatic leakage occurred in the 100 patients with BPJ.ConclusionBinding pancreaticojejunostomy procedure can effectively prevent the occurrance of anastomatic leakage and can be applied broadly.

Surgical Intervention for Hepatocellular Carcinoma with Bile Buct Thrombi

Objective: To summarize the experience of surgical intervention for hepatocellular carcinoma (HCC) with bile duct thrombi (BDT), and to evaluate the influence on prognosis.Methods: From 1994 to 2002, 15 patients with HCC and BDT who underwent surgical intervention were retrospectively analyzed.Results: The operative procedures included hepatectomy with removel of BDT (n=7), hepatectomy combined with extrahepatic bile duct resection (n=4), thrombectomy through choledochotomy (n=3), piggy back orthotopic liver transplantation (n=1). The 1- and 3-year survival rates were 73.3% and 40%, respectively. Two patients survived over 5 years.Conclusion: Surgical intervention was effective for patients with HCC and BDT. Operation for recurrent lesion can prolong survival period. Liver transplantation is a new treatment worthy of further investigation.