Liu Zhangxiong

Correlation Between Antioxidation and the Content of Total Phenolics and Anthocyanin in Black Soybean Accessions

The objective of this study was to investigate the correlations between antioxidations and the contents of the total phenolics and anthocyanin in 127 accessions of black soybean. A T-test, a fast clustering procedure, and a correlation coefficient analysis were used for experimentation. The variation ranges of the total antioxidant capacity (TAC), the total phenolics, and anthocyanin contents in 127 black soybean accessions were 0.44–3.56, 7.05–74.82, and 0.22–1.87 mg g−1, respectively, displaying significant genotype differences. The major differences in TAC, the total phenolics. and the anthocyanin contents existed among various types of accessions from geographical regions. The differences between the accessions from black and yellow soybeans, spring and autumn, summer and autumn, Dongbeichun and Nanfangchun, Dongbeichun and Nanfangxia, Beifangchun and Nanfangchun, and Beifangchun and Nanfangxia were significant at 0.01 or 0.05 levels, respectively. The general tendency was that the TAC, the total phenolics, and the anthocyanin contents of Beifangchun accessions were higher than that of Dongbeichun ones, while that of Nanfangchun accessions were the worst. 127 black soybean accessions could be clustered into 6 clusters, which consisted of 3, 24, 20, 31, 37, and 12 accessions, respectively. The most significant (P<0.01) correlations existed respectively between the TAC and the total phenolics content, and the TAC and the anthocyanin content of black soybean. The results showed that the total phenolics and anthocyanin in black soybean seed coat were the important antioxidation substances.

Analysis of introgressed segments in near-isogenic lines carrying soybean maturity genes.

It is very important to exploit the soybean maturity genes for developing varieties with high-yield and wide adaptability in both theory and practice. The objective of the study was to identify the size and position of introgressed segments associating with maturity genes, which provides information of fine mapping and cloning maturity genes. Twenty-three introgression lines (near isogenic lines, NILs) carrying four soybean maturity genes and their recurrent parent, were analyzed with 243 SSR markers, and 266 introgression segments were found, so each NIL containing 11.6 introgressed segments on average. The majority of 150 introgressed segments with E1, were located on chromosome 6, while 55, 49, 73 introgressed segments with E2, e3, E5 on chromosomes 20, 12 and 20 respectively. The NILs with the same maturity gene trended to cluster together. The results showed that E1 was related to the interval of satt643-sat_312 on chromosome 6 and sat_095 on chromosome 11; both E2 and E5 loci associated with the interval of satt587-satt496 on chromosome 20; e3 was related to the interval of satt317-satt181 on chromosome 12. Therefore, E1 locus was validated with NILs, and a new marker related to E1 was detected, and E2, e3, E5 gene-related markers were identified.