Liucai Sui
Anhui Agricultural University
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Featured researches published by Liucai Sui.
Zygote | 2012
Zubing Cao; Liucai Sui; Yunsheng Li; Suofei Ji; Xiaorong Zhang; Yunhai Zhang
The present study was to investigate if a completely chemically defined medium (PZM-4) could support the early development of porcine embryos derived from parthenogenetic activation (PA) and cloning (somatic cell nuclear transfer, SCNT), and to lay the foundation for determining the physiological roles of certain supplements in this medium. Porcine embryos derived from PA and SCNT were cultured in media: PZM-3 (a chemically semi-defined medium), PZM-4 (a fully defined medium), and PZM-5 (an undefined medium). Early embryo development was observed. We found that the three medium groups (PZM-3, PZM-4 and PZM-5) exhibited no significant differences in cleavage rates of PA embryos (p > 0.05), while the blastocyst rate in PZM-3 was significantly higher than in PZM-4 and PZM-5 (78.9% vs. 36.0% and 52.3%) (p < 0.05). Moreover, total cell number per blastocyst in PZM-3 was clearly higher than in PZM-5 but similar to that in PZM-4. As for SCNT embryos, no significant differences were observed for the cleavage rates or the blastocyst rates among the three groups (p > 0.05). However, total cell number per blastocyst in PZM-3 was notably higher than in PZM-5, but was similar to that in PZM-4. In conclusion, our results suggested that the completely chemically defined medium PZM-4 can be used to efficiently support the early development of porcine PA and SCNT embryos.
Italian Journal of Animal Science | 2014
Yunsheng Li; Chuanbiao Zhang; Yang Gao; Yuanliang Zhang; Liucai Sui; Xiaorong Zhang; Yunhai Zhang
The present study aimed to investigate the effects of epigallocatechin-3-gallate (EGCG) on the in vitro development of porcine oocytes, parthenogenetic activation embryos (PA), and somatic cell nuclear transfer (SCNT) embryos. In Experiment 1, 0 (control), 10, 30, and 50 μg/mL EGCG were added to in vitro maturation (IVM) medium to explore the effect of EGCG on IVM of pig oocytes. The matured oocytes were then used to produce PA and SCNT embryos. Either for nuclear maturation of oocytes or for the rates of cleavage and blastocyst of PA and SCNT embryos, no significant difference was found among all groups. However, the total cell number per cloned blastocyst was significantly lower in blastocysts derived from oocytes matured in 50 μg mL EGCG (P<0.05) as compared with the other groups. In Experiment 2, we cultured pig SCNT and PA embryos in medium containing various concentrations of EGCG to examine the effect of EGCG on preimplantation development. The cleavage and blastocyst rates and the total cell number per blastocyst did not significantly differ between PA and SCNT embryos among all groups. However, the reactive oxygen species level was significantly lower in the PA embryos cultured in 10 μg mL EGCG than the other groups (P<0.05). Our results suggest that high doses of EGCG in IVM are harmful to the oocytes as evidenced by the decreased quality of SCNT embryos, and EGCG has no beneficial effects on in vitro development of pig cloned embryos.
Scientific Reports | 2016
Qi Yao; Li Chen; Yuan-Jiao Liang; Liucai Sui; Li Guo; Jingwei Zhou; Kai Fan; Jun Jing; Yunhai Zhang; Bing Yao
Blastomere biopsy is an essential technique in preimplantation genetic diagnosis (PGD), a screening test that can detect genetic abnormalities of embryos before their transfer into uterus. Our results showed that the weights of fetuses derived from biopsied embryos were lower than that of non-biopsied counterparts at E12.5, E15.5, and E18.5. The ratio of fetal/placental (F/P) weights in the biopsied group was significantly lower than that in the non-biopsied group at E18.5. At E18.5, the mRNAs for selected glucose transporters, system A amino acid transporters, system L amino acid transporters, and imprinted genes were downregulated in the placentae of biopsied group, and the GLUT1 and CAT3 protein levels were decreased too. More apoptotic cells were detected by TUNEL in the placentae of biopsied group. Placentae from biopsied embryos exhibited lower levels of SOD and GSH. Furthermore, the concentration of MDA increased in the placentae from biopsied group. The levels of IL1B, IL6, and TNFA also significantly increased in the placentae of biopsied group. This study suggested that placental function may be sensitive to blastomere biopsy procedures, and placental oxidative stress and inflammation associated with blastomere biopsy may be critical factors of abnormal placental function and further influence the fetal development.
Biotechnology Letters | 2012
Tao Gui; Meiling Zhang; Jianwen Chen; Yuanliang Zhang; Naru Zhou; Yu Zhang; Jia Tao; Liucai Sui; Yunsheng Li; Ya Liu; Xiaorong Zhang; Yunhai Zhang
Archive | 2010
Zubing Cao; Yunsheng Li; Liucai Sui; Yunhai Zhang; Xiaorong Zhang
Archive | 2011
Yunsheng Li; Yunhai Zhang; Chunyang Han; Meiling Zhang; Liucai Sui; Hongguo Cao; Xiaorong Zhang; Ya Liu; Yong Tao; Yuanliang Zhang; Minghui Jiao; Suofei Ji; Qing Jia; Yu Zhang; Tao Gui; Naru Zhou; Xiaorui Liu; Pan Yang; Zijun Zhang; Jianping Ding
Archive | 2011
Meiling Zhang; Xiaorong Zhang; Yunhai Zhang; Yunsheng Li; Ya Liu; Yong Tao; Fugui Fang; Liucai Sui; Zijun Zhang; Jianping Ding; Hongguo Cao
Archive | 2011
Liucai Sui; Yunhai Zhang; Zongjun Yin; Yunsheng Li; Hongguo Cao; Xiaorong Zhang; Ya Liu; Yong Tao; Meiling Zhang; Yuanliang Zhang; Minghui Jiao; Suofei Ji; Qing Jia; Yu Zhang; Tao Gui; Xiaowei Qin; Naru Zhou; Xiaorui Liu; Wei Wang; Pan Yang; Zijun Zhang; Jianping Ding
Archive | 2010
Wenhao Lu; Yunhai Zhang; Xiaorong Zhang; Yunsheng Li; Hongguo Cao; Wei Wang; Zubing Cao; Ya Liu; Yong Tao; Fugui Fang; Liucai Sui; Ying Feng; Suofei Ji; Xuguang Liu; Zijun Zhang; Zongjun Yin; Jianping Ding
Reproduction, Fertility and Development | 2014
Meiling Zhang; Liucai Sui; Yunsheng Li; Zhen Chen; Yuanliang Zhang; T. Liu; J. Xu; Xiaorong Zhang