Lixia Sheng

Decitabine facilitates the generation and immunosuppressive function of regulatory γδT cells derived from human peripheral blood mononuclear cells

Decitabine facilitates the generation and immunosuppressive function of regulatory γδT cells derived from human peripheral blood mononuclear cells

Dasatinib promotes the potential of proliferation and antitumor responses of human γδT cells in a long-term induction ex vivo environment.

Dasatinib promotes the potential of proliferation and antitumor responses of human γδT cells in a long-term induction ex vivo environment

γδ T cell and other immune cells crosstalk in cellular immunity.

γδ T cells have been recognized as effectors with immunomodulatory functions in cellular immunity. These abilities enable them to interact with other immune cells, thus having the potential for treatment of various immune-mediated diseases with adoptive cell therapy. So far, the interactions between γδ T cell and other immune cells have not been well defined. Here we will discuss the interactivities among them and the perspective on γδ T cells for their use in immunotherapy could be imagined. The understanding of the crosstalk among the immune cells in immunopathology might be beneficial for the clinical application of γδ T cell.

Rapid mobilization of fully functional natural killer cells into blood by AMD3100

Plerixafor (AMD3100), a selective antagonist of CXCR4, effectively mobilizes CD34+ hematopoietic progenitor cells from the marrow to peripheral blood with minimal side effects. Varmavuo and colleagues showed that grafts mobilized with granulocyte–colony-stimulating factor (G-CSF) plus AMD3100 contained significantly more CD3–CD16/ 56+ natural killer (NK) cells when compared to G-CSF– mobilized grafts. Therefore, AMD3100 may have a favorable effect on NK-cell mobilization and NK cell–mediated antitumor effects, but whether AMD3100-mobilized NK cells are functionally competent has not been previously investigated. In this study, we used a mouse model to analyze the effects of AMD3100 on NK-cell function in vivo and ex vivo, both alone and in combination with G-CSF. Sixto 8-week-old CB6F1 mice (a cross between female BALB/c and male C57BL/6) were divided into four treatment groups: Group 1, subcutaneous injection of phosphate-buffered saline (PBS) as a control; Group 2, AMD-3100 (5 mg/kg), single dose; Group 3, G-CSF (250 mg/kg·day) for 5 days; and Group 4, AMD3100 plus G-CSF. Blood samples were taken from the tail vein for cell counting and flow cytometric analysis at baseline (before treatment) and at 1, 2, and 4 hours after treatment. Both AMD-3100 alone and AMD3100 plus G-CSF induced the mobilization of CD3e-DX5+ NK cells into the blood. The percentage and absolute number of NK cells increased after 1 hour and reached maximal values 2 hours after treatment with AMD3100 (with or without G-CSF), but not G-CSF alone (Fig. 1). Next, we analyzed NK-specific cytotoxicity in vivo after mobilization using 56-carboxyfluorescein diacetatesuccinimidyl ester (CFSE)-based detection. Splenocytes from CB6F1 mice and the parental C57BL/6 mice were labeled with 0.5 mmol/L (CFSE low) and 10 mmol/L CFSE (CFSE high), respectively, and transferred into CB6F1 mice 2 hours after the above-described treatments. We found that an increased proportion of parental target cells were