Liyuan Lv

Single Nucleotide Polymorphisms in Growth Hormone Gene and Their Association with Growth Traits in Siniperca chuatsi (Basilewsky)

Growth hormone (GH) has been considered as a candidate gene for growth traits in fish. In this study, polymorphisms of the GH gene were evaluated for associations with growth traits in 282 Siniperca chuatsi individuals. Using directly sequencing, four single nucleotide polymorphisms (SNPs) were identified in GH gene, with two mutations in intron 4 (g.4940A>C, g.4948A>T), one mutation in exon 5 (g.5045T>C) and one in intron 5 (g.5234T>G). Notably, three of them were significantly associated with growth performance, particularly for g.4940A>C which was highly correlated with all the four growth traits. In conclusion, our results demonstrated that these SNPs in GH gene could influence growth performance of S.chuatsi and could be used for marker-assisted selection (MAS) in this species.

The complete mitochondrial genome of the hybrid of Siniperca chuatsi (♀) × Siniperca scherzeri (♂)

Abstract In this study, we determined the complete mitochondrial DNA sequence of the hybrid of Siniperca chuatsi (♀) × Siniperca scherzeri (♂). The total length of the hybrid mitogenome is 16,497 bp, with the base composition of 28.61% A, 29.16% C, 16.23% G, and 26.00% T. It contains 2 rRNA genes, 13 protein-coding genes, 22 tRNA genes, and 1 control region. The composition and order of these genes are identical to most of other vertebrates. All the protein-coding genes start with ATG, except for COX1 with the initiation codon GTG. Three types of stop codons are used by the coding genes, including complete stop codons (TAA and TAG) and an incomplete stop codon (T). Three conserved domains were observed in the control region. The complete mitogenome of the hybrid of S. chuatsi (♀) × S. scherzeri (♂) could contribute to a better solution of the study in mitochondrial inheritance mechanism.

The complete mitochondrial genome sequence of Coreoperca whiteheadi (Perciformes: Serranidae)

Abstract In this paper, the complete mitochondrial DNA (mtDNA) sequence of Coreoperca whiteheadi was determined. The complete mtDNA genome sequence of C. whiteheadi is 16,483 bp in length. It consists of 13 protein-coding genes, 22 transfer RNA genes, 2 rRNA genes and 2 non-coding regions. Overall base composition of mitogenome is estimated to be 28.30% for A, 29.33% for C, 16.06% for G and 26.32% for T, respectively, with a high A + T content (54.62%). The complete mitogenome of the C. whiteheadi could contribute to basic researches on population history, molecular systematics and phylogeography. It is also helpful to the reasonable utilization and development of rational management strategies for C. whiteheadi resource.

The complete mitochondrial genome sequence of Siniperca undulate (Perciformes: Percichthyidae)

Abstract In this paper, the complete mitochondrial DNA (mtDNA) sequence of Siniperca undulate was determined. The complete mtDNA genome sequence of S. undulate was 16,504 bp in length. It consisted of 13 protein-coding genes, 22 transfer RNA genes, 2 rRNA genes and 2 non-coding regions. Overall base composition of mitogenome was estimated to be 28.38% for A, 29.43% for C, 16.46% for G and 25.73% for T, respectively, with a high A + T content (54.11%). The complete mitogenome of the S. undulate can provide a basic data for the studies on population history, molecular systematics, phylogeography, stock evaluation and conservation genetics. It is also helpful to the reasonable utilization and development of rational management strategies for S. undulate resource.

The complete mitochondrial genome of the hybrid ofSiniperca scherzeri(♀) × Siniperca chuatsi(♂)

Abstract In the present study, the complete mitogenome of the hybrid of Siniperca scherzeri (♀) × Siniperca chuatsi (♂) is determined to be 16,504 bp long and showed a typical vertebrate pattern with 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The base composition of heave strand in descending order is C (29.61%), A (28.31%), T (25.61%) and G (16.47%), with a slight AT bias of 53.92%. All the protein-coding genes are initiated by typical ATG codon, except for COX1 gene with the initiation codon GTG. Nine genes end with the complete stop codon TAA or TAG, while the COX1, COX2, ND4 and CYTB genes terminate with an incomplete stop codon T. The complete mitogenome of the hybrid of S. scherzeri (♀) × S. chuatsi (♂) could provide an important data set for the study in mitochondrial inheritance mechanism.

The complete mitochondrial genome of the hybrid of Siniperca kneri (♀) × Siniperca chuatsi (♂)

Abstract The complete mitochondrial genome sequence of the hybrid of Siniperca kneri (♀) × Siniperca chuatsi (♂) is described in this study. The 16,488 bp long circular molecule consisted of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region, showed a typical vertebrate pattern. The overall base composition of the heavy strand is 28.59% A, 29.20% C, 16.25% G, and 25.96% T, with a slight AT bias of 54.55%. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. There are 5 regions of gene overlap totaling 14 bp and 20 intergenic spacer regions totaling 91 bp. The complete mitogenome of the hybrid of S. kneri (♀) × S. chuatsi (♂) could provide an important data set for the study in mitochondrial inheritance mechanism.