Ljiljana Vapa

Polymorphism of mtDNA regions in brown hare (Lepus europaeus) populations from Vojvodina (Serbia and Montenegro)

It is well known that there is heterogeneity of mitochondrial DNA (mtDNA) within and among natural populations of same species. The polymorphism level of particular regions of mtDNA gives valuable results in detection of population genetic structure. The aim of this paper was to detect polymorphism of three mtDNA regions: cytochrome oxidase I (COI), Control region, and 12S/16S rRNA, by the mtDNA RFLP-PCR method, in three Lepus europaeus populations from Vojvodina province (Serbia and Montenegro). Polymorphism was detected within the two regions, COI and Control region, while 12S/16S rRNA region was monomorphic in all 77 individuals. Eight haplotypes were detected in the brown hare population in Vojvodina, and three were unique for the Srem brown hare population.

Genetic variability in brown hare (Lepus europeaus) populations in Yugoslavia

SummaryGenetic allozyme variability of brown hare populations in Vojvodina (Yugoslavia) was analyzed. Analyses were done by the method of polyacrylamide gel eleclrophoresis. The screening for the nine enzyme systems represented by 17 presumptive structural loci revealed polymorphism only inMdh-2, Idh-2, Pgd-2, Pgd-3, Pgm-2, Est- 1 andEst-2. The value of average heterozygosity (H) was 0.07, while that of polymorphism (P) 23.53. Neis values of genetic identity (1) ranged from 0.95 to 0.99. Apart from relatively high polymorphism and heterozygosity values, level of genetic identity was normal for the level of different local populations.ZusammenfassungDie genetische Allozym-Variabilität von Feldhasen-Populalionen in Jugoslawien wurde untersucht. Zur Analyse wurde die Polyacrylamid-Gel Elektrophorese benutzt. Die Überprüfung der 9 Enzymsysteme repräsentiert durch 17 vermutete Loci von Strukturgenen zeigten nur Polymorphismen in Mdh-2, Idh-2, Pgd-2, Pgd-3, Pgm-2, Est-1 Est-2. Der durchschnittliche Heterozygotiegrad (H) betrug 0,07, der für Polymorphic (P) war 23,53. Neis Werle für genetische Identität (I) lag zwischen 0,95–0,99. Abgesehen von relativ hohen Werten für Polymorphic und Heterozygotie war das Ausma\ der genetischen Identität für die verschiedenen lokalen Populationen normal.

Allozyme diversity in pheasants (Phasianus spp.) from breeding stations in Serbia

The pheasant breeds are widely used for restocking of natural populations depleted by hunting. The pheasant population number decline was detected during the 1970s in many hunting areas of Europe. One of its possible reasons might be the loss of adaptability in populations originating from breeding stations, which was caused by inbreeding depression. The aim of this paper was the analysis of genetic variability in pheasant populations from three breeding stations in Vojvodina province (Serbia) by means of allozyme diversity detection. The allozyme variability analysis of pheasants from all three breeding stations revealed polymorphisms at nine loci: Ldh-1, Mor-1, Mor-2, Es-1, Mod-2, Pgd, Gpi-2, Odh, and Sod. The analysis of individuals from three different breeding stations showed mean values of observed heterozygosity of Ho=0.137, polymorphism P95%=30%, and H/P ratio H/P=0.430, which indicate a normal level of genetic variability for bird populations. Comparative analysis of three pheasant populations showed a high level of interpopulation differentiation.